Engineered ketocarotenoid biosynthesis in the polyextremophilic red microalga Cyanidioschyzon merolae 10D

Seger, Mark; Mammadova, Fakhriyya; Villegas-Valencia, Melany; Freitas, Bárbara Bastos de; Chang, Clarissa; Isachsen, Iona; Hemstreet, Haley; Abualsaud, Fatimah; Boring, Malia; Lammers, Peter J.; Lauersen, Kyle J.

doi:10.1016/j.mec.2023.e00226

Cited by 7 publications

(2 citation statements)

References 52 publications

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“…Simultaneous enhancement of fucoxanthin and β-carotene was achieved with a similar approach in the diatom Phaeodactylum tricornutum (Bacillariophyceae) through overexpressing endogenous biosynthetic genes [224,225] and a plastoglobule protein to augment pigment sequestration [226]. Ketocarotenoids engineering was recently reported in the industrially relevant species C. merolae via heterologous expression of two biosynthetic genes [227], and in the thraustochytrid A. limacinum via overexpression of an endogenous β-carotene hydroxylase gene [228].…”

Section: Biomanufacturing Of Immunomodulatory Metabolites 41 Engineer...mentioning

confidence: 99%

The Clinical Promise of Microalgae in Rheumatoid Arthritis: From Natural Compounds to Recombinant Therapeutics

Cutolo,

Caferri,

Campitiello

et al. 2023

Marine Drugs

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Rheumatoid arthritis (RA) is an invalidating chronic autoimmune disorder characterized by joint inflammation and progressive bone damage. Dietary intervention is an important component in the treatment of RA to mitigate oxidative stress, a major pathogenic driver of the disease. Alongside traditional sources of antioxidants, microalgae—a diverse group of photosynthetic prokaryotes and eukaryotes—are emerging as anti-inflammatory and immunomodulatory food supplements. Several species accumulate therapeutic metabolites—mainly lipids and pigments—which interfere in the pro-inflammatory pathways involved in RA and other chronic inflammatory conditions. The advancement of the clinical uses of microalgae requires the continuous exploration of phytoplankton biodiversity and chemodiversity, followed by the domestication of wild strains into reliable producers of said metabolites. In addition, the tractability of microalgal genomes offers unprecedented possibilities to establish photosynthetic microbes as light-driven biofactories of heterologous immunotherapeutics. Here, we review the evidence-based anti-inflammatory mechanisms of microalgal metabolites and provide a detailed coverage of the genetic engineering strategies to enhance the yields of endogenous compounds and to develop innovative bioproducts.

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Section: Biomanufacturing Of Immunomodulatory Metabolites 41 Engineer...mentioning

confidence: 99%

The Clinical Promise of Microalgae in Rheumatoid Arthritis: From Natural Compounds to Recombinant Therapeutics

Cutolo,

Caferri,

Campitiello

et al. 2023

Marine Drugs

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show abstract

“…This species is extremophilic, with optimal laboratory growth conditions including low pH (~ 2) and high temperatures (~ 42 °C) (1,2). C. merolae and other thermo-acidophilic red algae draw interest for their unique biology and simple characteristics, which position them as useful model organisms and as candidates for biotechnology applications (3)(4)(5)(6). For example, C. merolae is of interest because it is one of few organisms which relies on photosynthesis in geothermal spring environments, where hot and acidic conditions restrict the availability of inorganic carbon and challenge biological carbon fixation (1,7).…”

mentioning

confidence: 99%

Modeling With Uncertainty Quantification Identifies Essential Features of a Non-Canonical Algal Carbon-Concentrating Mechanism

Steensma,

Kaste,

Heo

et al. 2024

Preprint

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The thermoacidophilic red alga Cyanidioschyzon merolae survives its challenging environment likely in part by operating a carbon-concentrating mechanism (CCM). Here, we demonstrated that cellular affinity of C. merolae for CO2 is stronger than the rubisco affinity of C. merolae for CO2. This provided further evidence that C. merolae operates a CCM while lacking structures and functions characteristic of CCMs in other organisms. To test how such a CCM could function, we created a mathematical compartmental model of a simple CCM distinct from those previously described in detail. The results supported the feasibility of this proposed minimal and non-canonical CCM in C. merolae. To facilitate robust modeling of this process, we incorporated new physiological and enzymatic data into the model, and we additionally trained a surrogate machine-learning model to emulate the mechanistic model and characterized the effects of model parameters on key outputs. This parameter exploration enabled us to identify model features that influenced whether the model met experimentally-derived criteria for functional carbon-concentration and efficient energy usage. Such parameters included cytosolic pH, bicarbonate pumping cost and kinetics, cell radius, carboxylation velocity, number of thylakoid membranes, and CO2 membrane permeability. Our exploration thus suggested that a novel CCM could exist in C. merolae and illuminated essential features necessary for CCMs to function.

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Green extraction of pigment from astaxanthin-producing algae using natural deep eutectic solvents

Al Fuhaid,

Wellman,

Kharbatia

et al. 2024

Algal Research

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Engineered ketocarotenoid biosynthesis in the polyextremophilic red microalga Cyanidioschyzon merolae 10D

Cited by 7 publications

References 52 publications

The Clinical Promise of Microalgae in Rheumatoid Arthritis: From Natural Compounds to Recombinant Therapeutics

The Clinical Promise of Microalgae in Rheumatoid Arthritis: From Natural Compounds to Recombinant Therapeutics

Modeling With Uncertainty Quantification Identifies Essential Features of a Non-Canonical Algal Carbon-Concentrating Mechanism

Green extraction of pigment from astaxanthin-producing algae using natural deep eutectic solvents

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