Engineered heat treated methanogenic granules: A promising biotechnological approach for extreme thermophilic biohydrogen production

Abreu, A. A.; Alves, J. I.; Pereira, M. A.; Karakashev, Dimitar Borisov; Alves, M. M.; Angelidaki, Irini

doi:10.1016/j.biortech.2010.07.070

Cited by 54 publications

(27 citation statements)

References 39 publications

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“…Differences in substrate composition did not affect the bacterial community in reactors R arab and R gluc and similarity index between Arab/Gluc samples at the end of the operation was as high as 94%. Predominant DGGE bands in R gluc and R arab were identical to the ones present in the inoculum used in this study and for which the phylogeny had been previously assessed [6]. Two of the predominant DGGE bands showed high similarity (> 99%) with the hydrogen-producing Thermoanaerobacterium thermosaccharolyticum .…”

Section: Resultssupporting

confidence: 66%

“… DGGE profile of granular sludge samples from a reactor fed with arabinose and glucose [6] and at Day 27 and Day 41 from arabinose (and glucose reactors .…”

Section: Resultsmentioning

confidence: 99%

“…Efficient microbial fermentation of hexoses and pentoses is, therefore, the key step for hydrogen production from plant biomass. However, combined fermentation of mixtures of hexoses and pentoses is often prevented due to catabolic repression; in the presence of glucose, pentoses might be converted to a lesser extent thereby decreasing overall fermentation yields [5,6]. Moreover, efficient hydrogen production from sugars is dependent on the different possible fermentation pathways (Figure 1).…”

Section: Introductionmentioning

confidence: 99%

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Biohydrogen production from arabinose and glucose using extreme thermophilic anaerobic mixed cultures

Abreu

Karakashev

Angelidaki

et al. 2012

Biotechnol Biofuels

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BackgroundSecond generation hydrogen fermentation technologies using organic agricultural and forestry wastes are emerging. The efficient microbial fermentation of hexoses and pentoses resulting from the pretreatment of lingocellulosic materials is essential for the success of these processes.ResultsConversion of arabinose and glucose to hydrogen, by extreme thermophilic, anaerobic, mixed cultures was studied in continuous (70°C, pH 5.5) and batch (70°C, pH 5.5 and pH 7) assays. Two expanded granular sludge bed (EGSB) reactors, Rarab and Rgluc, were continuously fed with arabinose and glucose, respectively. No significant differences in reactor performance were observed for arabinose and glucose organic loading rates (OLR) ranging from 4.3 to 7.1 kgCOD m-3 d-1. However, for an OLR of 14.2 kgCOD m-3 d-1, hydrogen production rate and hydrogen yield were higher in Rarab than in Rgluc (average hydrogen production rate of 3.2 and 2.0 LH2 L-1 d-1 and hydrogen yield of 1.10 and 0.75 molH2 mol-1substrate for Rarab and Rgluc, respectively). Lower hydrogen production in Rgluc was associated with higher lactate production. Denaturing gradient gel electrophoresis (DGGE) results revealed no significant difference on the bacterial community composition between operational periods and between the reactors. Increased hydrogen production was observed in batch experiments when hydrogen partial pressure was kept low, both with arabinose and glucose as substrate. Sugars were completely consumed and hydrogen production stimulated (62% higher) when pH 7 was used instead of pH 5.5.ConclusionsContinuous hydrogen production rate from arabinose was significantly higher than from glucose, when higher organic loading rate was used. The effect of hydrogen partial pressure on hydrogen production from glucose in batch mode was related to the extent of sugar utilization and not to the efficiency of substrate conversion to hydrogen. Furthermore, at pH 7.0, sugars uptake, hydrogen production and yield were higher than at pH 5.5, with both arabinose and glucose as substrates.

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Section: Resultssupporting

confidence: 66%

“… DGGE profile of granular sludge samples from a reactor fed with arabinose and glucose [6] and at Day 27 and Day 41 from arabinose (and glucose reactors .…”

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Biohydrogen production from arabinose and glucose using extreme thermophilic anaerobic mixed cultures

Abreu

Karakashev

Angelidaki

et al. 2012

Biotechnol Biofuels

Self Cite

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“…Pearson's similarity coefficient was calculated using BioNumerics  software, version 2.5. The Shannon-Wiener diversity index was calculated based on the intensity of the DGGE bands and on the height of the densitometric curve peaks, according to Abreu et al (2010).…”

Section: Discussionmentioning

confidence: 99%

Distribution of Archaeal and Bacterial communities in a subtropical reservoir

et al. 2015

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Aim: Microbial communities play a central role in environmental process such as organic matter mineralization and the nutrient cycling process in aquatic ecosystems. Despite their ecological importance, variability of the structure of archaeal and bacterial communities in freshwater remains understudied. Methods: In the present study we investigated the richness and density of archaea and bacteria in the water column and sediments of the Itupararanga Reservoir. We also evaluated the relationship between the communities and the biotic and abiotic characteristics. Samples were taken at five depths in the water column next to the dam and three depths next to the reservoir entrance. Results: PCR-DGGE evaluation of the archaeal and bacterial communities showed that both were present in the water column, even in oxygenated conditions. Conclusions: The density of the bacteria (qPCR) was greater than that of the archaea, a result of the higher metabolic plasticity of bacteria compared with archaea.Keywords: freshwater; microbial communities; PCR/DGGE; real time PCR.Resumo: Objetivo: As comunidades microbianas desempenham papel crucial nos processos ambientais como na mineralização de matéria orgânica e ciclagem de nutrientes em ambientes aquáticos. No entanto, apesar da importância ecológica de archaea e bactéria em ambientes aquáticos, a estrutura destas comunidades permanece pouco estudada. Métodos: Neste sentido, nos avaliamos a riqueza e densidade de archaea e bactéria na coluna d'água e no sedimento do reservatório de Itupararanga e avaliamos as relações entre os fatores bióticos e abióticos. Para tanto, amostragens foram realizadas em cinco profundidades na região próxima a barragem e em três profundidades no ponto da entrada. Resultados: A partir de PCR-DGGE observou-se que ambas as comunidades distribuíram-se ao logo dos pontos de amostragem, mesmo em condições com presença de oxigênio. Conclusões: A densidade bacterianas, avaliada por qPCR foi maior do que a de archaea, provavelmente devido a maior plasticidade metabólica desses microrganismos.Palavras-chave: águas continentais; comunidade microbiana; PCR/DGGE; real time PCR. Acta Limnologica Brasiliensia

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“…Multivariate DGGE analyzes of the band profiles were performed by the BioNumeric 2.5 software (Applied Maths, Belgium), which were analyzed using the Pearson correlation. The dendrograms were constructed by UPMA (Unweighted Pair Group Method with Arithmetic Averages) method and diversity indices were calculated using ShannoneWiener (H 0 ) (Abreu et al, 2010).…”

Section: Pcr-dggementioning

confidence: 99%

Methanogenic potential of an anaerobic sludge in the presence of anionic and nonionic surfactants

Motteran

Braga

Sakamoto

et al. 2014

International Biodeterioration & Biodegradation

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Engineered heat treated methanogenic granules: A promising biotechnological approach for extreme thermophilic biohydrogen production

Cited by 54 publications

References 39 publications

Biohydrogen production from arabinose and glucose using extreme thermophilic anaerobic mixed cultures

Biohydrogen production from arabinose and glucose using extreme thermophilic anaerobic mixed cultures

Distribution of Archaeal and Bacterial communities in a subtropical reservoir

Methanogenic potential of an anaerobic sludge in the presence of anionic and nonionic surfactants

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