Engineered green alga Chlamydomonas reinhardtii as a whole-cell photosynthetic biocatalyst for stepwise photoproduction of H₂ and ε-caprolactone

Abstract

Photosynthetic whole-cell biocatalysts are promising platforms for direct production of solar chemicals. Here, we employed the green microalga Chlamydomonas reinhardtii (hereafter Chlamydomonas) as a heterologous host for the cyclohexanone monooxygenase...

Employing photocurable biopolymers to engineer photosynthetic 3D-printed living materials for production of chemicals

“…The entrapped cells produced ε-caprolactone within the printed matrix with a comparable specific activity (3.46 ± 0.2 U g DCW −1 ) to the biotransformation conducted in suspension cultures (3.6 ± 0.8 U g DCW −1 ), 30 while the product distribution remained consistent, with 87% of the product formed. During the biotransformation, only a slight increase in the Chl content was detected (Fig.…”

“…This engineered strain is capable of biotransformation of cyclohexanone to ε-caprolactone via a heterologously expressed cyclohexanone monooxygenase (CHMO) from Acinetobacter NCIB 9871. 30 In this reaction, the substrate is exogenously provided to the cells. Consequently, it is anticipated that the substrate diffuses into cells, while the product is secreted out into the medium.…”

“…ε-caprolactone is another compound utilised by the polymer industry for the synthesis of different plastic products. 30 We showed that the proposed composite material comprising Alg, GGMMA and LAP is compatible with photosynthetic cells, is suitable for 3D-printing of mechanically stable matrices including thin biofilms and does not hinder the production of target chemicals.…”

“…Synechocystis PCC 6803 efe strain expressing ethylene forming enzyme (Efe) 27 was used for ethylene production and Chlamydomonas reinhardtii UVM11-CW/CHMO_PSAD clone 10 ( Chlamydomonas ) 30 was used for the biotransformation of cyclohexanone to ε-caprolactone.…”

“…ε-caprolactone is another compound utilised by the polymer industry for the synthesis of different plastic products. 30…”