Engineered extracellular vesicles with high collagen-binding affinity present superior <i>in situ</i> retention and therapeutic efficacy in tissue repair

Hao, Dake; Lu, Lu; Song, Hengyue; Duan, Yixin; Chen, Jianing; Carney, Randy P.; Li, Jian Jian; Zhou, Ping; Nolta, Jan A.; Lam, Kit S.; Leach, J. Kent; Farmer, Diana L.; Panitch, Alyssa; Wang, Aijun

doi:10.7150/thno.70448

Cited by 19 publications

(22 citation statements)

References 81 publications

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“…While a previous report used flow cytometry to test the interaction of rat pancreatic cancer EVs with single ECM components, including collagen I [ 17 ], another report used an electron microscopy-based approached to show that EVs released by activated neutrophils physically interact with collagen I fibers and that they can degrade collagen, contributing to the pathogenesis of chronic obstructive pulmonary disease (COPD), a disease of lung ECM remodeling [ 21 ]. In addition, a recent study showed that human placental MSC-derived EVs were able to bind collagen-coated surfaces and that EVs could be conjugated to a collagen-binding peptide to increase their affinity for collagen binding [ 22 ]. Hence, EVs appear to interact with the ECM, and this interaction would likely contribute to ECM remodeling during different pathological processes.…”

Section: Discussionmentioning

confidence: 99%

“…As shown here, this protocol could be transposed to other EV subpopulations of interest (e.g., EVs from different pathological stages, or EVs bearing or not specific collagen receptors such as integrins) and provide a basis to investigate the role of EVs in functional assays such as invasion assays and matrix remodeling. In addition, the functionalization of therapeutic EVs to bind collagen [ 22 ] opens new perspectives to use our conclusions and protocols in therapeutic assays.…”

Section: Discussionmentioning

confidence: 99%

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In Vitro Interaction of Melanoma-Derived Extracellular Vesicles with Collagen

Palmulli

Bresteau

Raposo

et al. 2023

IJMS

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Extracellular vesicles are now considered as active contributors to melanoma progression through their capacity to modify the tumor microenvironment and to favor the formation of a pre-metastatic niche. These prometastatic roles of tumor-derived EVs would pass through their interaction with the extracellular matrix (ECM) and its remodeling, in turn providing a substrate favoring persistent tumor cell migration. Nevertheless, the capacity of EVs to directly interact with ECM components is still questionable. In this study, we use electron microscopy and a pull-down assay to test the capacity of sEVs, derived from different melanoma cell lines, to physically interact with collagen I. We were able to generate collagen fibrils coated with sEVs and to show that melanoma cells release subpopulations of sEVs that can differentially interact with collagen.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

In Vitro Interaction of Melanoma-Derived Extracellular Vesicles with Collagen

Palmulli

Bresteau

Raposo

et al. 2023

IJMS

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“…EVs with transfection of IL-10 overexpressing vector plasmids can modulate T cell immunity in autoimmune uveitis [ 96 ]. The chemical engineering of EVs to express a collagen-binding peptide could reduce inflammation and induce muscle regeneration in ischemic disease [ 97 ]. The incubation of EVs with anti-angiogenic peptide KV11-anchoring peptide CP05 with EVs could suppress neovascularization in the retina [ 98 ].…”

Section: Types Of Sev Therapeuticsmentioning

confidence: 99%

Small Extracellular Vesicles as a New Class of Medicines

Lee

Choi²,

Shin

et al. 2023

Pharmaceutics

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Extracellular vesicles (EVs) are nanovesicles that are naturally released from cells in a lipid bilayer-bound form. A subset population with a size of 200 nm, small EVs (sEVs), is enticing in many ways. Initially perceived as mere waste receptacles, sEVs have revealed other biological functions, such as cell-to-cell signal transduction and communication. Besides their notable biological functions, sEVs have profound advantages as future drug modalities: (i) excellent biocompatibility, (ii) high stability, and (iii) the potential to carry undruggable macromolecules as cargo. Indeed, many biopharmaceutical companies are utilizing sEVs, not only as diagnostic biomarkers but as therapeutic drugs. However, as all inchoate fields are challenging, there are limitations and hindrances in the clinical translation of sEV therapeutics. In this review, we summarize different types of sEV therapeutics, future improvements, and current strategies in large-scale production.

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“…In addition, collagen in the hydrogel is responsible for the retention of loaded EVs. This retention permits a sustained release of EVs that are an optimum characteristic for a biomaterial in their use with EVs [ 70 ].…”

Section: Suitable Ev-based Hydrogels For Wound Healing Therapiesmentioning

confidence: 99%

“…All these studies have achieved a synergic association between EVs and hydrogels in terms of their functional and wound-healing properties [ 70 , 71 , 72 , 73 ]. However, as mentioned before, EV conservation requires improvement before reaching the clinics.…”

Section: Suitable Ev-based Hydrogels For Wound Healing Therapiesmentioning

confidence: 99%

Extracellular Vesicle-Based Hydrogels for Wound Healing Applications

Amengual-Tugores

Ráez-Meseguer

Forteza-Genestra

et al. 2023

IJMS

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Hydrogels and extracellular vesicle-based therapies have been proposed as emerging therapeutic assets in wound closure. The combination of these elements has given good results in managing chronic and acute wounds. The intrinsic characteristics of the hydrogels in which the extracellular vesicles (EVs) are loaded allow for overcoming barriers, such as the sustained and controlled release of EVs and the maintenance of the pH for their conservation. In addition, EVs can be obtained from different sources and through several isolation methods. However, some barriers must be overcome to transfer this type of therapy to the clinic, for example, the production of hydrogels containing functional EVs and identifying long-term storage conditions for EVs. The aim of this review is to describe the reported EV-based hydrogel combinations, along with the obtained results, and analyze future perspectives.

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Engineered extracellular vesicles with high collagen-binding affinity present superior in situ retention and therapeutic efficacy in tissue repair

Cited by 19 publications

References 81 publications

In Vitro Interaction of Melanoma-Derived Extracellular Vesicles with Collagen

In Vitro Interaction of Melanoma-Derived Extracellular Vesicles with Collagen

Small Extracellular Vesicles as a New Class of Medicines

Extracellular Vesicle-Based Hydrogels for Wound Healing Applications

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