Engineered Corynebacterium glutamicum as the Platform for the Production of Aromatic Aldehydes

Abstract

Graphical Abstract

Para‑hydroxybenzaldehyde against transient focal cerebral ischemia in rats via mitochondrial preservation

“…Although a previous pharmacokinetic study showed that PHBA in GE has brain targeting activity during drug distribution (50), its effect and mechanism on transient focal cerebral ischemia remains unclear. At present, PHBA can already be synthesized artificially (51). The preliminary experiment of our research group confirmed that the commercially available PHBA is consistent with the PHBA isolated from GE (52).…”

l-Serine Biosensor-Controlled Fermentative Production of l-Tryptophan Derivatives by Corynebacterium glutamicum

“…Albeit lacking a TrpR homolog, C. glutamicum has a putative operator sequence with similarity to the operator DNA binding sequence of the TrpR repressor of E. coli and was detected upstream of the −10 promoter region of the trp operon [26,27]. Transcription of the trp operon is controlled by attenuation and deletion of the leader peptide gene trpL and improved overproduction of L-tryptophan [28] and aromatic aldehydes [29]. Several L-tryptophan-producing C. glutamicum strains have been developed [30][31][32].…”

Comparative Analysis of Catabolic and Anabolic Dehydroshikimate Dehydratases for 3,4-DHBA Production in Escherichia coli

“…Another biosynthetic pathway for 3,4-DHBA production was proposed through the end product of the common aromatic pathway chorismate [8] (Figure 1). This biosynthetic route includes more reactions than 3,4-DHBA synthesis via DSD reaction but has other advantages [8]. DSDs are a diverse group of enzymes, which are subdivided into four classes: bacterial single-domain, fungal single-domain, bacterial two-domain, and bacterial membrane-associated enzymes [9].…”

“…DHS is an intermediate of the common aromatic pathway ( Figure 1 ), and the DSD reaction allows to synthesize 3,4-DHBA from glucose [ 7 ] ( Figure 1 ). Another biosynthetic pathway for 3,4-DHBA production was proposed through the end product of the common aromatic pathway chorismate [ 8 ] ( Figure 1 ). This biosynthetic route includes more reactions than 3,4-DHBA synthesis via DSD reaction but has other advantages [ 8 ].…”

“…Another biosynthetic pathway for 3,4-DHBA production was proposed through the end product of the common aromatic pathway chorismate [ 8 ] ( Figure 1 ). This biosynthetic route includes more reactions than 3,4-DHBA synthesis via DSD reaction but has other advantages [ 8 ].…”