Engineered collagen hydrogels for the sustained release of biomolecules and imaging agents: promoting the growth of human gingival cells

Choi, Jonghoon; Park, Hoyoung; Kim, Taeho; Jeong, Yoon; Oh, Man Hwan; Hyeon, Taeghwan; Gilad, Assaf A.; Lee, Kwan H.

doi:10.2147/ijn.s71304

Cited by 22 publications

(11 citation statements)

References 34 publications

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“…In this study, in contrast, we were interested in understanding the flow of molecules/drugs directly out of the gel, and the influence of the embedded fibroblasts and endothelial cells in fibrin gels on the release profile of FD 250 kDa. FD was selected as a model due to its wide range of molecular weights, high stability, and frequent use in drug release studies and diffusion analysis [ 19 , 33 , 34 ]. FD 250 kDa has an approximate radius of 11 nm, which is comparable to the size of growth factors, such as nerve growth factor (130 kDa, globular radius ~3 nm, unfolded ~12 nm) and hepatocyte growth factor (80 kDa, globular radius ~3 nm, unfolded ~9 nm) [ 35 , 36 , 37 ].…”

Section: Discussionmentioning

confidence: 99%

FITC-Dextran Release from Cell-Embedded Fibrin Hydrogels

et al. 2021

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Fibrin hydrogel is a central biological material in tissue engineering and drug delivery applications. As such, fibrin is typically combined with cells and biomolecules targeted to the regenerated tissue. Previous studies have analyzed the release of different molecules from fibrin hydrogels; however, the effect of embedded cells on the release profile has yet to be quantitatively explored. This study focused on the release of Fluorescein isothiocyanate (FITC)-dextran (FD) 250 kDa from fibrin hydrogels, populated with different concentrations of fibroblast or endothelial cells, during a 48-h observation period. The addition of cells to fibrin gels decreased the overall release by a small percentage (by 7–15% for fibroblasts and 6–8% for endothelial cells) relative to acellular gels. The release profile was shown to be modulated by various cellular activities, including gel degradation and physical obstruction to diffusion. Cell-generated forces and matrix deformation (i.e., densification and fiber alignment) were not found to significantly influence the release profiles. This knowledge is expected to improve fibrin integration in tissue engineering and drug delivery applications by enabling predictions and ways to modulate the release profiles of various biomolecules.

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Section: Discussionmentioning

confidence: 99%

FITC-Dextran Release from Cell-Embedded Fibrin Hydrogels

et al. 2021

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“…The degradation or protein release data in Figure 5 B show that all constructs were fully degraded within 8 h of incubation with type I collagenase, which indicates efficient degradation for sustained release of the proteins [ 57 ]. Another study has also shown that collagen constructs incubated at 37 °C and 5% CO 2 for 24 h released 20–70% of the biomolecules encapsulated in the construct [ 58 ]. In the present study, the degradability properties of the constructs are expected to facilitate the sustained release of the encapsulated DFCM proteins to the target area to stimulate wound healing [ 57 ].…”

Section: Discussionmentioning

confidence: 99%

Hybrid Collagen Hydrogel/Chondroitin-4-Sulphate Fortified with Dermal Fibroblast Conditioned Medium for Skin Therapeutic Application

et al. 2021

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The current strategy for rapid wound healing treatment involves combining a biomaterial and cell-secreted proteins or biomolecules. This study was aimed at characterizing 3-dimensional (3D) collagen hydrogels fortified with dermal fibroblast-conditioned medium (DFCM) as a readily available acellular skin substitute. Confluent fibroblasts were cultured with serum-free keratinocyte-specific medium (KM1 and KM2) and fibroblast-specific medium (FM) to obtain DFCM. Subsequently, the DFCM was mixed with collagen (Col) hydrogel and chondroitin-4-sulphate (C4S) to fabricate 3D constructs termed Col/C4S/DFCM-KM1, Col/C4S/DFCM-KM2, and Col/C4S/DFCM-FM. The constructs successfully formed soft, semi-solid and translucent hydrogels within 1 h of incubation at 37 °C with strength of <2.5 Newton (N). The Col/C4S/DFCM demonstrated significantly lower turbidity compared to the control groups. The Col/C4S/DFCM also showed a lower percentage of porosity (KM1: 35.15 ± 9.76%; KM2: 6.85 ± 1.60%; FM: 14.14 ± 7.65%) compared to the Col (105.14 ± 11.87%) and Col/C4S (143.44 ± 27.72%) constructs. There were no changes in both swelling and degradation among all constructs. Fourier transform infrared spectrometry showed that all groups consisted of oxygen–hydrogen bonds (O-H) and amide I, II, and III. In conclusion, the Col/C4S/DFCM constructs maintain the characteristics of native collagen and can synergistically deliver essential biomolecules for future use in skin therapeutic applications.

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“…These cell processes are regulated by the interaction of collagen with integrins in cell surface receptors 74 . Moreover, collagen has garnered attention as a delivery system for the sustained release of different drugs and chemical agents 75 , 76 .…”

Section: Discussionmentioning

confidence: 99%

Improving motor neuron-like cell differentiation of hEnSCs by the combination of epothilone B loaded PCL microspheres in optimized 3D collagen hydrogel

Mahmoodi

Hassannejad

et al. 2021

Sci Rep

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Spinal cord regeneration is limited due to various obstacles and complex pathophysiological events after injury. Combination therapy is one approach that recently garnered attention for spinal cord injury (SCI) recovery. A composite of three-dimensional (3D) collagen hydrogel containing epothilone B (EpoB)-loaded polycaprolactone (PCL) microspheres (2.5 ng/mg, 10 ng/mg, and 40 ng/mg EpoB/PCL) were fabricated and optimized to improve motor neuron (MN) differentiation efficacy of human endometrial stem cells (hEnSCs). The microspheres were characterized using liquid chromatography-mass/mass spectrometry (LC-mas/mas) to assess the drug release and scanning electron microscope (SEM) for morphological assessment. hEnSCs were isolated, then characterized by flow cytometry, and seeded on the optimized 3D composite. Based on cell morphology and proliferation, cross-linked collagen hydrogels with and without 2.5 ng/mg EpoB loaded PCL microspheres were selected as the optimized formulations to compare the effect of EpoB release on MN differentiation. After differentiation, the expression of MN markers was estimated by real-time PCR and immunofluorescence (IF). The collagen hydrogel containing the EpoB group had the highest HB9 and ISL-1 expression and the longest neurite elongation. Providing a 3D permissive environment with EpoB, significantly improves MN-like cell differentiation and maturation of hEnSCs and is a promising approach to replace lost neurons after SCI.

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Engineered collagen hydrogels for the sustained release of biomolecules and imaging agents: promoting the growth of human gingival cells

Cited by 22 publications

References 34 publications

FITC-Dextran Release from Cell-Embedded Fibrin Hydrogels

FITC-Dextran Release from Cell-Embedded Fibrin Hydrogels

Hybrid Collagen Hydrogel/Chondroitin-4-Sulphate Fortified with Dermal Fibroblast Conditioned Medium for Skin Therapeutic Application

Improving motor neuron-like cell differentiation of hEnSCs by the combination of epothilone B loaded PCL microspheres in optimized 3D collagen hydrogel

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