Engineered bio-nanocapsules, the selective vector for drug delivery system

Yu, Dongwei; Fukuda, Takayuki; Tuoya,; Kuroda, Shinya; Tanizawa, Katsuyuki; Kondo, Akihiko; Ueda, Mitsuharu; Yamada, Tesshi; Tada, Hayato; Seno, Masaharu

doi:10.1080/15216540500484368

Cited by 12 publications

(6 citation statements)

References 39 publications

(37 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is considered that ZZ‐BNC is an efficient nano‐machine for molecular targeting in drug delivery system displaying antibodies on the surface [25, 29, 45]. Multivalency of EC‐Fc was successfully demonstrated to target ErbB2 enhancing the internalization of ErbB2, in this report.…”

Section: Discussionmentioning

confidence: 72%

Enhanced internalization of ErbB2 in SK-BR-3 cells with multivalent forms of an artificial ligand

Vaidyanath¹,

Hashizume²,

Nagaoka³

et al. 2011

Journal of Cellular and Molecular Medicine

Self Cite

View full text Add to dashboard Cite

Targeting and down-regulation of ErbB2, a member of EGF receptor family, is regarded as one of the key aspect for cancer treatment because it is often overexpressed in breast and ovarian cancer cells. Although natural ligands for ErbB2 have not been found, unlike other ErbB receptors, EC-1, a 20-amino acid circular peptide, has been shown to bind to ErbB2 as an artificial ligand. Previously we showed EC-1 peptide did not induce the internalization of ErbB2 in SK-BR-3 cells. In this report, we designed divalent and multivalent forms of EC-1 peptide with the Fc portion of the human IgG and bionanocapsule modified with ZZ-tag on its surface to improve the interaction with ErbB2. These forms showed higher affinity to ErbB2 than that of EC-1 monomer. Furthermore, prominent endosomal accumulation of ErbB2 occurred in SK-BR-3 cells when stimulated with EC-Fc ligand multivalently displayed on the surface of the bionanocapsule, whereas SK-BR-3 cells as themselves displayed stringent mechanism against ErbB2 internalization without stimulation. The multivalent form of EC-1 peptide appeared to internalize ErbB2 more efficiently than divalent form did. This internalization was unaffected by the inhibition of clathrin association, but inhibited when the cholesterol was depleted which explained either caveolar or GPI-AP-early endocytic compartment (GEEC) pathway. Because of the lack of caveolin-1 expression, caveolar machinery may be lost in SK-BR-3 cell line. Therefore, it is suggested that the multivalent form of EC-1 induces the internalization of ErbB2 through the GEEC pathway.

show abstract

Section: Discussionmentioning

confidence: 72%

Enhanced internalization of ErbB2 in SK-BR-3 cells with multivalent forms of an artificial ligand

Vaidyanath¹,

Hashizume²,

Nagaoka³

et al. 2011

Journal of Cellular and Molecular Medicine

Self Cite

View full text Add to dashboard Cite

show abstract

“…Due to the presence of L protein, BNC can specifically deliver genes or drugs to hepatocytes. [222][223][224][225] The BNC exhibits a reliable safety profile as HBV L protein and surface antigen have already been proven to be safe as an immunogen of hepatitis B vaccine in humans. BNC shows high transfection efficiency that is equivalent to the original HBV.…”

Section: Bionanocapsulesmentioning

confidence: 99%

Applications of Nanoparticles for Anticancer Drug Delivery: A Review

Zhu¹,

Liao²

2015

j nanosci nanotechnol

129

View full text Add to dashboard Cite

Biodegradable nanometer-sized particles have novel structural and physical properties that are attracting great interests from pharmaceuticals for the targeted delivery of anticancer drugs and imaging contrast agents. These smart nanoparticles are designed to ferry chemotherapeutic agents or therapeutic genes into malignant cells while sparing healthy cells. In this review, we describe currently clinically used chemotherapeutics in nanoparticle formulation and discuss the current status of nanoparticles developed as targeting delivery systems for anticancer drugs, with emphasis on formulations of micelles, liposome, polymeric nanoparticles, gold nanoparticle dendrimers, and bionanocapsules.

show abstract

“…When these particles penetrate into the living body, they will play various roles in biomedical and clinical applications. Recently inorganic spherical particles in micro-and nano-size are actively prepared by various methods for the applications to biomedical uses [8][9][10][11]. Silica particles are the most representative inorganic substance of these applications [12][13][14].…”

Section: Introductionmentioning

confidence: 99%