Engineered bacteria for valorizing lignocellulosic biomass into bioethanol

Panahi, Hamed Kazemi Shariat; Dehhaghi, Mona; Dehhaghi, Somayeh; Guillemin, Gilles J.; Lam, Su Shiung; Aghbashlo, Mortaza; Tabatabaei, Meisam

doi:10.1016/j.biortech.2021.126212

Cited by 18 publications

(6 citation statements)

References 90 publications

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“…This requirement stems from each microorganism's inability to synthesise certain metabolites at the gene level [44]. The development of ethanologenic bacteria can be accomplished in three ways: (i) by replacing or introducing heterogeneous genes from a potent ethanol-producing strain; (ii) by overexpressing the native genes which are responsible for ethanol synthesis; and (iii) by eliminating native metabolic pathways, they could compete with ethanol production (e.g., hydrogen and organic acids) [46].…”

Section: Methodologies For Enhanced Bioethanol Productionmentioning

confidence: 99%

Bioconversion of Starch Base Food Waste into Bioethanol

et al. 2022

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The global demand for fuel keeps increasing daily. The massive depletion of fossil fuels and their influence on the environment as pollution is a severe problem. Meanwhile, food waste disposal is also a complex problem in solid-waste management since one-third of every food consumed is discarded as waste. The standard waste management methods, including food waste incineration and landfilling, are considered hazardous to the environment. Food waste constituents are majorly starch-based and contain various biomolecules, including sugar, lipids, proteins, vitamins, cellulose, etc. These polysaccharides can be hydrolysed into monosaccharides such as glucose, which can then be fermented using microorganisms to produce ethanol through the fermenting of sugars derived from enzymatic hydrolysis treatment of food wastes. The human food system is rich in starch, which can be a potential resource for bioethanol production.

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Section: Methodologies For Enhanced Bioethanol Productionmentioning

confidence: 99%

Bioconversion of Starch Base Food Waste into Bioethanol

et al. 2022

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“…1 DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, WI 53726, USA. 2 Department of Bacteriology, University of Wisconsin-Madison, Madison, WI, USA. 3 Genome Center of Wisconsin, Madison, WI, USA.…”

Section: Supplementary Informationmentioning

confidence: 99%

“…The production of fuels and chemicals from plant biomass by microbes presents a sustainable alternative to fossil resources [ 1 , 2 ]. However, for this process to be economically viable, high yields and titers are required [ 3 – 5 ].…”

Section: Introductionmentioning

confidence: 99%

Lipid membrane remodeling and metabolic response during isobutanol and ethanol exposure in Zymomonas mobilis

Rivera Vazquez,

Trujillo,

Williams

et al. 2024

Biotechnol Biofuels

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Background Recent engineering efforts have targeted the ethanologenic bacterium Zymomonas mobilis for isobutanol production. However, significant hurdles remain due this organism’s vulnerability to isobutanol toxicity, adversely affecting its growth and productivity. The limited understanding of the physiological impacts of isobutanol on Z. mobilis constrains our ability to overcome these production barriers. Results We utilized a systems-level approach comprising LC–MS/MS-based lipidomics, metabolomics, and shotgun proteomics, to investigate how exposure to ethanol and isobutanol impact the lipid membrane composition and overall physiology of Z. mobilis. Our analysis revealed significant and distinct alterations in membrane phospholipid and fatty acid composition resulting from ethanol and isobutanol exposure. Notably, ethanol exposure increased membrane cyclopropane fatty acid content and expression of cyclopropane fatty acid (CFA) synthase. Surprisingly, isobutanol decreased cyclopropane fatty acid content despite robust upregulation of CFA synthase. Overexpression of the native Z. mobilis’ CFA synthase increased cyclopropane fatty acid content in all phospholipid classes and was associated with a significant improvement in growth rates in the presence of added ethanol and isobutanol. Heterologous expression of CFA synthase from Clostridium acetobutylicum resulted in a near complete replacement of unsaturated fatty acids with cyclopropane fatty acids, affecting all lipid classes. However, this did not translate to improved growth rates under isobutanol exposure. Correlating with its greater susceptibility to isobutanol, Z. mobilis exhibited more pronounced alterations in its proteome, metabolome, and overall cell morphology—including cell swelling and formation of intracellular protein aggregates —when exposed to isobutanol compared to ethanol. Isobutanol triggered a broad stress response marked by the upregulation of heat shock proteins, efflux transporters, DNA repair systems, and the downregulation of cell motility proteins. Isobutanol also elicited widespread dysregulation of Z. mobilis’ primary metabolism evidenced by increased levels of nucleotide degradation intermediates and the depletion of biosynthetic and glycolytic intermediates. Conclusions This study provides a comprehensive, systems-level evaluation of the impact of ethanol and isobutanol exposure on the lipid membrane composition and overall physiology of Z. mobilis. These findings will guide engineering of Z. mobilis towards the creation of isobutanol-tolerant strains that can serve as robust platforms for the industrial production of isobutanol from lignocellulosic sugars.

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“…These have also been given preference to metabolic pathways manipulation because of their substrate suitability, fast growth rate, and well-tractable physiology and genetics. The user-friendly hosts, that is, Zymomonas mobilis and E. coli, Clostridium , etc., have been the center of recent metabolic engineering studies to develop a model microbial chassis for sustainable biorefinery practices in the modern era [ 84 ].…”

Section: Recent Metabolic Advances In Microorganism For Biofuel Produ...mentioning

confidence: 99%

Recent advances in metabolic engineering of microorganisms for advancing lignocellulose-derived biofuels

et al. 2022

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Combating climate change and ensuring energy supply to a rapidly growing global population has highlighted the need to replace petroleum fuels with clean, and sustainable renewable fuels. Biofuels offer a solution to safeguard energy security with reduced ecological footprint and process economics. Over the past years, lignocellulosic biomass has become the most preferred raw material for the production of biofuels, such as fuel, alcohol, biodiesel, and biohydrogen. However, the cost-effective conversion of lignocellulose into biofuels remains an unsolved challenge at the industrial scale. Recently, intensive efforts have been made in lignocellulose feedstock and microbial engineering to address this problem. By improving the biological pathways leading to the polysaccharide, lignin, and lipid biosynthesis, limited success has been achieved, and still needs to improve sustainable biofuel production. Impressive success is being achieved by the retouring metabolic pathways of different microbial hosts. Several robust phenotypes, mostly from bacteria and yeast domains, have been successfully constructed with improved substrate spectrum, product yield and sturdiness against hydrolysate toxins. Cyanobacteria is also being explored for metabolic advancement in recent years, however, it also remained underdeveloped to generate commercialized biofuels. The bacterium Escherichia coli and yeast Saccharomyces cerevisiae strains are also being engineered to have cell surfaces displaying hydrolytic enzymes, which holds much promise for near-term scale-up and biorefinery use. Looking forward, future advances to achieve economically feasible production of lignocellulosic-based biofuels with special focus on designing more efficient metabolic pathways coupled with screening, and engineering of novel enzymes.

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Engineered bacteria for valorizing lignocellulosic biomass into bioethanol

Cited by 18 publications

References 90 publications

Bioconversion of Starch Base Food Waste into Bioethanol

Bioconversion of Starch Base Food Waste into Bioethanol

Lipid membrane remodeling and metabolic response during isobutanol and ethanol exposure in Zymomonas mobilis

Recent advances in metabolic engineering of microorganisms for advancing lignocellulose-derived biofuels

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