Engineered Bacillus subtilis biofilms as living glues

Zhang, Chen; Huang, Jiaofang; Zhang, Jicong; Liu, Suying; Cui, Mengkui; An, Bo; Wang, Xinyu; Pu, Jiahua; Zhao, Tianxin; Chen, Fan; Lu, Timothy K.; Zhong, Chao

doi:10.1016/j.mattod.2018.12.039

Cited by 87 publications

(70 citation statements)

References 52 publications

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“…Glue Tyrosinase Expression of tyrosinase in biofilm-based adhesives improved adhesive properties through production of DOPA-quinones which subsequently cross-linked. [188] Abbreviations: CoA, coenzyme A; ECM, extracellular matrix; HRP, horseradish peroxidase; PEG, poly(ethylene glycol). tuneable gelation rates under physiologically relevant conditions.…”

Section: Transglutaminasementioning

confidence: 99%

Enzyme‐catalysed polymer cross‐linking: Biocatalytic tools for chemical biology, materials science and beyond

et al. 2020

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Intermolecular cross-linking is one of the most important techniques that can be used to fundamentally alter the material properties of a polymer. The introduction of covalent bonds between individual polymer chains creates 3D macromolecular assemblies with enhanced mechanical properties and greater chemical or thermal tolerances. In contrast to many chemical cross-linking reactions, which are the basis of thermoset plastics, enzyme catalysed processes offer a complimentary paradigm for the assembly of cross-linked polymer networks through their predictability and high levels of control. Additionally, enzyme catalysed reactions offer an inherently 'greener' and more biocompatible approach to covalent bond formation, which could include the use of aqueous solvents, ambient temperatures, and heavy metal-free reagents. Here, we review recent progress in the development of biocatalytic methods for polymer cross-linking, with a specific focus on the most promising candidate enzyme classes and their underlying catalytic mechanisms. We also provide exemplars of the use of enzyme catalysed cross-linking reactions in industrially relevant applications, noting the limitations of these approaches and outlining strategies to mitigate reported deficiencies.

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Section: Transglutaminasementioning

confidence: 99%

Enzyme‐catalysed polymer cross‐linking: Biocatalytic tools for chemical biology, materials science and beyond

et al. 2020

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“…Such structures are characterized by interlaced fibers for increase of contact area [ 18 ]. By using microbial genetic engineering, several studies have designed a new generation of marine-inspired adhesives that combine two or more independent natural adhesion systems, but their methods of synthesis are complex, low-yield and prone to microbial exposure [ 19 , 20 ]. As an alternative and flexible approach, molecular mimicry can be utilized to engineer molecules that share structural and functional similarities to the originals, and this has been applied in fields ranging from chemical synthesis [ 21 ] to autoimmune activation [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

Marine-inspired molecular mimicry generates a drug-free, but immunogenic hydrogel adhesive protecting surgical anastomosis

Huang

Jiang

Liu

et al. 2021

Bioactive Materials

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Herein, we report the synthesis of a biomimic hydrogel adhesive that addresses the poor healing of surgical anastomosis. Dopamine-conjugated xanthan gum (Da-g-Xan) is fabricated using deep insights into the molecular similarity between mussels' adhesive and dopamine as well as the structural similarity between barnacle cement proteins and xanthan gum. The hydrogel mimics marine animals’ adherence to wet tissue surfaces. Upon applying this adhesive to colonic anastomosis in a rat model, protective effects were shown by significantly improving the bursting pressure. Mechanistically, the architecture of Da-g-Xan hydrogel is maintained by dynamic intermolecular hydrogen bonds that allow the quick release of Da-g-Xan. The free Da-g-Xan can regulate the inflammatory status and induce type 2 macrophage polarization (M2) by specifically interacting with mannose receptors (CD206) revealed by RNA-sequencing and molecular binding assays. Consequently, an appropriate microenvironment for tissue healing is created by the secretion of chemokines and growth factors from M2 macrophages, strengthening the fibroblast migration and proliferation, collagen synthesis and epithelial vascularization. Overall, this study demonstrates an unprecedented strategy for generating an adhesive by synergistic mimicry inspired by two marine animals, and the results show that the Da-g-Xan adhesive augments native tissue regenerative responses, thus enabling enhanced recovery following surgical anastomosis.

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“…Significantly, compared to the conventional one-pot method, encapsulation efficiency (EE %, defined as the proportion of ZIF-90 crystals containing encapsulated cells to all the ZIF-90 particles, including those none cell-encapsulated empty ZIF-90 crystals in the system) was substantially improved to 61.9 ± 5.2% from 21.3 ± 4.4% through a multiple-step deposition (MSD) method. This multi-step deposition approach for high EE of MOF-based biocomposites can be applied for microencapsulation of other types of cells other than E. coli [ [40] , [41] , [42] ], providing a new approach to synthesize hybrid living materials with higher environment tolerance.…”

Section: Introductionmentioning

confidence: 99%

Encapsulation of bacterial cells in cytoprotective ZIF-90 crystals as living composites

Kang

et al. 2021

Materials Today Bio

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Exploiting metal-organic frameworks (MOFs) as selectively permeable shelters for encapsulating engineered cells to form hybrid living materials has attracted increasing attention in recent years. Optimizing the synthesis process to improve encapsulation efficiency (EE) is critical for further technological development and applications. Here, using ZIF-90 and genetically engineered Escherichia coli (E. coli) as a demo, we fabricated E. coli @ZIF-90 living composites in which E. coli cells were encapsulated in ZIF-90 crystals. We illustrated that ZIF-90 could serve as a protective porous cage for cells to shield against toxic bactericides including benzaldehyde, cinnamaldehyde, and kanamycin. Notably, the E. coli cells remained alive and could self-reproduce after removing the ZIF-90 crystal cages in ethylenediaminetetraacetic acid, suggesting a feasible route for protecting and prolonging the lifespan of bacterial cells. Moreover, an aqueous multiple-step deposition approach was developed to improve EE of the E. coli @ZIF-90 composites: the EE increased to 61.9 ± 5.2%, in contrast with the efficiency of the traditional method (21.3 ± 4.4%) prepared with PBS buffer. In short, we develop a simple yet viable strategy to manufacture MOF-based living hybrid materials that promise new applications across diverse fields.

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Engineered Bacillus subtilis biofilms as living glues

Cited by 87 publications

References 52 publications

Enzyme‐catalysed polymer cross‐linking: Biocatalytic tools for chemical biology, materials science and beyond

Enzyme‐catalysed polymer cross‐linking: Biocatalytic tools for chemical biology, materials science and beyond

Marine-inspired molecular mimicry generates a drug-free, but immunogenic hydrogel adhesive protecting surgical anastomosis

Encapsulation of bacterial cells in cytoprotective ZIF-90 crystals as living composites

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