Engineered acellular collagen scaffold for endogenous cell guidance, a novel approach in urethral regeneration

Pinnagoda, Kalitha; Larsson, Hans M.; Vythilingam, Ganesh; Vardar, Elif; Engelhardt, Eva; Thambidorai, Rajendrarao C.; Hubbell, Jeffrey A.; Frey, Peter

doi:10.1016/j.actbio.2016.07.033

Cited by 42 publications

(50 citation statements)

References 22 publications

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“…Such scaffolds have been shown to be capable of accelerating bone repair and regeneration . Similarly, high‐density collagen tubes engineered from rat‐tail collagen have been suggested as urethral grafts for partial urethra replacement or as a scaffold for urethra generation in cases of congenital malformations or injuries . Although concerted efforts continue to design bioinspired biomaterials or to engineer acellular biological scaffolds, isolated components presented within the native ECM have emerged as an integral part of raw materials for regenerative therapy and tissue‐engineering applications .…”

Section: Recreating Cell–ecm Interactions In a Cell Homementioning

confidence: 99%

Engineering a Cell Home for Stem Cell Homing and Accommodation

Yin

et al. 2017

Adv. Biosys.

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Distilling complexity to advance regenerative medicine from laboratory animals to humans, in situ regeneration will continue to evolve using biomaterial strategies to drive endogenous cells within the human body for therapeutic purposes; this approach avoids the need for delivering ex vivo‐expanded cellular materials. Ensuring the recruitment of a significant number of reparative cells from an endogenous source to the site of interest is the first step toward achieving success. Subsequently, making the “cell home” cell‐friendly by recapitulating the natural extracellular matrix (ECM) in terms of its chemistry, structure, dynamics, and function, and targeting specific aspects of the native stem cell niche (e.g., cell–ECM and cell–cell interactions) to program and steer the fates of those recruited stem cells play equally crucial roles in yielding a therapeutically regenerative solution. This review addresses the key aspects of material‐guided cell homing and the engineering of novel biomaterials with desirable ECM composition, surface topography, biochemistry, and mechanical properties that can present both biochemical and physical cues required for in situ tissue regeneration. This growing body of knowledge will likely become a design basis for the development of regenerative biomaterials for, but not limited to, future in situ tissue engineering and regeneration.

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Section: Recreating Cell–ecm Interactions In a Cell Homementioning

confidence: 99%

Engineering a Cell Home for Stem Cell Homing and Accommodation

Yin

et al. 2017

Adv. Biosys.

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“…1.85 mL). The neutralized solution was set in a mold previously used for manufacturing tubular constructs of 3 mm inner diameter 10 . To fabricate the LD-graft, the gelated collagen was manually compressed by rolling it on filter paper, followed by an air-drying step (Fig.…”

Section: Methodsmentioning

confidence: 99%

“…Burst pressure, ultimate tensile strength (UTS) and Young’s modulus of grafts (N = 4 in each group) were measured with an electronic manometer (Extech instruments HD750) or an Instron tensile machine (Norwood, MA, USA) as described previously 10 .…”

Section: Methodsmentioning

confidence: 99%

“…For example, mesenchymal stem cells are known to differentiate into different cell lineages, depending on the substrate stiffness 8 . Previous studies have shown that fluid content or collagen fiber density relates to the stiffness and influenced material properties of the collagen gels 9 , 10 . The more extracted fluid from the collagen gels, the stiffer the collagen material gets.…”

Section: Introductionmentioning

confidence: 99%

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Fiber density of collagen grafts impacts rabbit urethral regeneration

Larsson

Vythilingam

Pinnagoda

et al. 2018

Sci Rep

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There is a need for efficient and “off-the-shelf” grafts in urethral reconstructive surgery. Currently available surgical techniques require harvesting of grafts from autologous sites, with increased risk of surgical complications and added patient discomfort. Therefore, a cost-effective and cell-free graft with adequate regenerative potential has a great chance to be translated into clinical practice. Tubular cell-free collagen grafts were prepared by varying the collagen density and fiber distribution, thereby creating a polarized low fiber density collagen graft (LD-graft). A uniform, high fiber density collagen graft (HD-graft) was engineered as a control. These two grafts were implanted to bridge a 2 cm long iatrogenic urethral defect in a rabbit model. Histology revealed that rabbits implanted with the LD-graft had a better smooth muscle regeneration compared to the HD-graft. The overall functional outcome assessed by contrast voiding cystourethrography showed patency of the urethra in 90% for the LD-graft and in 66.6% for the HD-graft. Functional regeneration of the rabbit implanted with the LD-graft could further be demonstrated by successful mating, resulting in healthy offspring. In conclusion, cell-free low-density polarized collagen grafts show better urethral regeneration than high-density collagen grafts.

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“…Tissue engineering (TE) is an emerging field offering the possibility of providing true biological substitutes with patient-specific properties to restore the structure and function of pathologically altered tissues. Several groups have attempted TE urethral substitution by using acellular matrices such as Bladder Acellular Matrix Graft (BAMG) and Small Intestinal Submucosa (SIS), or cellularized matrices [23][24][25][26][27][28][29][30][31][32]. These matrices are prepared from native tissues by decellularizing and sterilising it.…”

Section: Current Tissue Engineering Strategies For Urologic Tissue Rementioning

confidence: 99%

Surgical Correction of Genitourinary Disorders Using the Self-Assembly Tissue Engineering

Pellerin¹,

Christophe²,

Grammond³

et al. 2019

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Urologic and gynaecologic patients often manifest congenital and/or acquired tissue and organ dysfunctions that require surgical reconstruction to recreate the normal genitourinary system functions. Such reconstruction is still a challenge due to the limited availability of suitable tissues, especially for severe urethral and vaginal replacement. Traditional intervention methods have varying degrees of donor site morbidity or inherent side effects. Interestingly, tissue engineering is a growing field that aims to replace or regenerate these dysfunctional tissues and organs with autologous cells, biomaterials, or a combination of both. Experience gained from tissue engineering suggests that the use of acellular matrices alone is not successful in supporting tissue growth over large surfaces. Cellular constituents need to be isolated, cultured and grown in vitro on scaffolds, then transplanted in vivo, in order to achieve successful three-dimensional tissue regeneration. Biomaterials are the backbone for cell-seeded reconstruction of the genitourinary tissues. Several research teams have explored a different cell culture approach based on self-assembly. This innovative technique relies on the capacity of cells cultured in the presence of ascorbate to secrete and deposit their own extracellular matrix forming a tissue-like substance. The mechanical and physical strength properties of these reconstructed tissues are similar to that of natural native tissues in certain models. Tissue engineered substitutes for urethral and vaginal mucosa were produced and subcutaneously grafted with success opening the way to new therapeutic strategies to correct genitourinary defects.

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Engineered acellular collagen scaffold for endogenous cell guidance, a novel approach in urethral regeneration

Cited by 42 publications

References 22 publications

Engineering a Cell Home for Stem Cell Homing and Accommodation

Engineering a Cell Home for Stem Cell Homing and Accommodation

Fiber density of collagen grafts impacts rabbit urethral regeneration

Surgical Correction of Genitourinary Disorders Using the Self-Assembly Tissue Engineering

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