Energy Transfer Fluorescent Labels for DNA Sequencing and Analysis

Xie, Jin; Hung, Su-Chun; Glazer, Alexander N.; Mathies, Richard A.

doi:10.1007/0-306-47947-8_3

Cited by 2 publications

(4 citation statements)

References 43 publications

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“…Indeed, ET primer technology has made a significant contribution to the Human Genome Project [34,59]. DNA sequencing has been further enhanced by the recent development of ET dye-labeled terminator chemistry [60][61][62].…”

Section: Energy-transfer Cassette Chemistrymentioning

confidence: 99%

“…An ET cassette allowing the ET-labeling of any primer sequence was developed as a partial answer to this limitation [53]. In addition, biotinylated ET cassettes and ET-labeled streptavidin moieties have also been synthesized and demonstrated [60]. Still, these procedures required the addition of the ET cassette onto the 5'-end of the primer during automated synthesis and post-synthetic dye conjugation [53].…”

Section: Energy-transfer Cassette Chemistrymentioning

confidence: 99%

“…ET cassette primer-labeling might now be used in assays where multiplexing and high sensitivity are required, including microarray hybridization formats, other DNA based assays, as well as protein and cell labeling [8,60,66,67]. Additionally, the ability to label any sequencing primer with an ET cassette label will facilitate custom sequencing methods and plasmid vector formats beyond those containing the universal M13 sequences.…”

Section: Energy-transfer Cassette Chemistrymentioning

confidence: 99%

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High-performance genetic analysis using microfabricated capillary array electrophoresis microplates

et al. 2001

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This review focuses on some recent advances in realizing microfabricated capillary array electrophoresis (microCAE). In particular, the development of a novel rotary scanning confocal fluorescence detector has facilitated the high-speed collection of sequencing and genotyping data from radially formatted microCAE devices. The concomitant development of a convenient energy-transfer cassette labeling chemistry allows sensitive multicolor labeling of any DNA genotyping or sequencing analyte. High-performance hereditary haemochromatosis and short tandem repeat genotyping assays are demonstrated on these devices along with rapid mitochondrial DNA sequence polymorphism analysis. Progress in supporting technology such as robotic fluid dispensing and batched data analysis is also presented. The ultimate goal is to develop a parallel analysis platform capable of integrated sample preparation and automated electrophoretic analysis with a throughput 10-100 times that of current technology.

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Section: Energy-transfer Cassette Chemistrymentioning

confidence: 99%

Section: Energy-transfer Cassette Chemistrymentioning

confidence: 99%

Section: Energy-transfer Cassette Chemistrymentioning

confidence: 99%

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High-performance genetic analysis using microfabricated capillary array electrophoresis microplates

et al. 2001

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“…Over the past 5 years, the introduction of energy transfer (ET) 2 fluorescent labels has provided a decadic improvement in DNA detection sensitivity (1)(2)(3)(4)(5). In particular, compared to single-dye-labeled primers, ET primers offer the advantages of excitation at a common wavelength, high acceptor fluorescence emission, and matched electrophoretic mobilities of the labeled sequencing extension fragments (6).…”

mentioning

confidence: 99%