Energy Transduction Inside of Amphiphilic Vesicles: Encapsulation of Photochemically Active Semiconducting Particles

Abstract

Amphiphilic bilayer membrane structures (vesicles) have been postulated to have been abiotically formed and spontaneously assemble on the prebiotic Earth, providing compartmentalization for the origin of life. These vesicles are similar to modern cellular membranes and can serve to contain water-soluble species, concentrate species, and have the potential to catalyze reactions. The origin of the use of photochemical energy in metabolism (i.e. energy transduction) is one of the central issues in the origin of life. This includes such questions as how energy transduction may have occurred before complex enzymatic systems, such as required by contemporary photosynthesis, had developed and how simple a photochemical system is possible. It has been postulated that vesicle structures developed the ability to capture and transduce light, providing energy for reactions. It has also been shown that pH gradients across the membrane surface can be photochemically created, but coupling these to drive chemical reactions has been difficult. Colloidal semiconducting mineral particles are known to photochemically drive redox chemistry. We propose that encapsulation of these particles has the potential to provide a source of energy transduction inside vesicles, and thereby drive protocellular chemistry, and represents a model system for early photosynthesis. In our experiments we show that TiO2 particles, in the approximately 20 nm size range, can be incorporated into vesicles and retain their photoactivity through the dehydration/rehydration cycles that have been shown to concentrate species inside a vesicle.

Lipids as Universal Biomarkers of Extraterrestrial Life

“…In principle, such processes would involve light-absorbing pigments either encapsulated as solutes or partitioned into boundary lipid membranes. For instance, encapsulated TiO 2 particles have been shown to reduce the redox indicator MV +2 or NAD + (to NADH) upon irradiation (Summers et al, 2009;Summers et al, 2010).…”

Supramolecular effect of curcurbit[7]uril on the binding mode of 2-(4-(dimethylamino) styryl)-1-methylpyridinium iodide with Calf Thymus DNA: From minor groove to intercalative

“…1(b)). The hypochromism effect in DASPMI absorption in presence of cucurbit medium points toward intercalative binding of DASPMI within the DNA base pairs [14,26]. The red shift indicates the binding of the DASPMI in the non-polar region of the DNA i.e., within the base pairs.…”

Hydrothermal Conditions and the Origin of Cellular Life

“…In principle, such processes would involve light-absorbing pigments either encapsulated as solutes or partitioned into boundary lipid membranes. For instance, encapsulated TiO 2 particles have been shown to reduce the redox indicator MV +2 or NAD + (to NADH) upon irradiation (Summers et al, 2009;Summers et al, 2010). Moreover, aqueous suspensions of the mineral sphalerite (ZnS) can photo-reduce CO 2 to formate when illuminated with near-UV light (Zhou and Guzman, 2014), providing a mechanism of prebiotic carbon fixation.…”