Currently, the most accurate and non-invasive method used to assess energy expenditure (EE) in sport horses is based on heart rate (HR) monitoring. However EE assessment using inertial platforms has been lately discussed in human sports medicine. The objective of this study was to evaluate whether inertial platforms would be useful tools to assess EE in horses.Six show-jumping and riding school horses (Thoroughbred and warmblood) were equipped with a HR monitoring system and a wireless inertial platform. Acceleration, HR and speed were measured during the exercise protocol that included walk, trot, canter and a sequence of 4 jumps. Stride maximum and minimum acceleration, and acceleration amplitude and root mean squares (RMS) were determined. Energy expenditure and oxygen uptake (VO 2 ) were calculated using HR and speed respectively. Bivariate correlations (non-parametric Spearman's ρ correlation) between EE, VO 2 and acceleration variables were tested.Spearman's ρ correlation was positive between both EE and VO 2 , and maximum acceleration, acceleration amplitude and RMS and negative for minimum acceleration. Acceleration variables of vertical and lateral movement were generally better correlated with EE and VO 2 (p < 0.001) than those of forward movement (p < 0.01).The results of this innovative approach reveal that the determination of EE in horses could be assessed using inertial platforms. Moreover vertical and lateral movements appear to influence more EE than forward movement.