Energy substrate metabolism, mitochondrial structure and oxidative stress after cardiac ischemia-reperfusion in mice lacking UCP3

Sánchez-Pérez, Patricia; Mata, Ana M.; Torp, May-Kristin; López-Bernardo, Elia; Heiestad, Christina Mathisen; Aronsen, Jan Magnus; Molina-Iracheta, Antonio; Jiménez-Borreguero, Luis Jesús; García-Rovés, Pablo M.; Costa, Ana S.H.; Frezza, Christian; Murphy, Michael P.; Stensløkken, Kåre‐Olav; Cadenas, Susana

doi:10.1016/j.freeradbiomed.2023.05.014

Cited by 7 publications

(3 citation statements)

References 86 publications

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“…Surgical removal of the right kidney was performed via laparotomy under anesthesia (mixture of 2-3% isoflurane and 97-98% oxygen and with mask ventilation) 3,9 . At 16 weeks post-UNX, rats underwent cardiac I/R injury surgical procedure 10,11 . At week 20, rats were sacrificed, the renal tissue was removed, fixed and preserved in paraffin.…”

Section: Methodology 21 Surgical Induction Of Cardiorenal Syndrome In...mentioning

confidence: 99%

“…Subsequently, the spectra were vector-normalized in the 900-1800 cm -1 range. The investigation of renal tissues also encompassed a preliminary Hierarchical Clustering Analysis (HLA) in the 900-1450 cm −1 range, employed to explore the complexity of the tissue, and investigate the chemical composition of different functional structures (vessel walls, glomeruli, and medullary regions) 11 . The Amide I and II signals were excluded from the HCA due to their high intensity, which might compromise the sensitivity of the analysis towards signals within the lower frequency range.…”

Section: Processing Of Ftir Spectra and Statistical Analysismentioning

confidence: 99%

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Exploring cardiorenal syndrome in rats: insights from vibrational spectroscopy techniques

Stefani,

Alunni Cardinali,

Justo da Silva

et al. 2024

Biomedical Spectroscopy, Microscopy, and Imaging III

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Clinical evidence underscores the intricate interplay between the heart and kidneys, where dysfunction in one organ contributes to progressive failure of both. In our previous work, using vibrational spectroscopy techniques, we demonstrated molecular changes in cardiac tissue post-uninephrectomy (UNX) and ischemia-reperfusion (I/R) surgeries in a rat cardiorenal syndrome (CS) model. It is now imperative to investigate whether structural changes in renal tissue following these two interventions are detectable.Vibrational spectroscopy serves as a powerful analytical technique with a fundamental role in molecular structure analysis. This method provides valuable insights into the intricate architecture of biomolecules, including proteins, lipids, and carbohydrates. Fourier-transform infrared (FTIR) microspectroscopy, in particular, emerges as a potent method for highresolution chemical imaging of various biological tissues, facilitating the analysis of molecular signatures indicative of physiological or pathological states.Despite the recognized utility of FTIR in various biomedical applications, its potential in assessing cardiorenal diseaseinduced lesions in heart and kidney tissues remains underexplored. Therefore, this study aims to bridge this gap by applying FTIR-imaging to identify spectroscopic markers related to renal complications. By characterizing the molecular fingerprints associated with pathological alterations in kidney tissue, this study aims to contribute to the development of non-invasive diagnostic tool for early detection and monitoring of renal dysfunction following surgical interventions.The findings of this study hold promise in advancing our understanding of the molecular mechanisms underlying renal complications, thereby facilitating timely interventions and the development of personalized therapeutic strategies in clinical settings.

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Section: Methodology 21 Surgical Induction Of Cardiorenal Syndrome In...mentioning

confidence: 99%

Section: Processing Of Ftir Spectra and Statistical Analysismentioning

confidence: 99%

Exploring cardiorenal syndrome in rats: insights from vibrational spectroscopy techniques

Stefani,

Alunni Cardinali,

Justo da Silva

et al. 2024

Biomedical Spectroscopy, Microscopy, and Imaging III

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“…Figure 2 studies conducted in diabetic rodents indicate that high glucose enhance superoxide generation and mitochondrial structural changes that increase the vulnerability of the myocardium to IR injury (78), and treatments that have antioxidant property attenuate myocardial IRI through improving mitochondrial homeostasis (28). Thus, excessive oxidative stress and impaired mitochondrial biogenesis in diabetic conditions rendered the diabetic heart more vulnerable to ischemic insults (56,79).…”

Section: High Glucose Induced Increase In Ros In the Heartmentioning

confidence: 99%

Salvianolic acids and its potential for cardio-protection against myocardial ischemic reperfusion injury in diabetes

Jiang,

Cai,

Han

et al. 2024

Front. Endocrinol.

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The incidence of diabetes and related mortality rate increase yearly in modern cities. Additionally, elevated glucose levels can result in an increase of reactive oxygen species (ROS), ferroptosis, and the disruption of protective pathways in the heart. These factors collectively heighten the vulnerability of diabetic individuals to myocardial ischemia. Reperfusion therapies have been effectively used in clinical practice. There are limitations to the current clinical methods used to treat myocardial ischemia-reperfusion injury. As a result, reducing post-treatment ischemia/reperfusion injury remains a challenge. Therefore, efforts are underway to provide more efficient therapy. Salvia miltiorrhiza Bunge (Danshen) has been used for centuries in ancient China to treat cardiovascular diseases (CVD) with rare side effects. Salvianolic acid is a water-soluble phenolic compound with potent antioxidant properties and has the greatest hydrophilic property in Danshen. It has recently been discovered that salvianolic acids A (SAA) and B (SAB) are capable of inhibiting apoptosis by targeting the JNK/Akt pathway and the NF-κB pathway, respectively. This review delves into the most recent discoveries regarding the therapeutic and cardioprotective benefits of salvianolic acid for individuals with diabetes. Salvianolic acid shows great potential in myocardial protection in diabetes mellitus. A thorough understanding of the protective mechanism of salvianolic acid could expand its potential uses in developing medicines for treating diabetes mellitus related myocardial ischemia-reperfusion.

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Human mitochondrial uncoupling protein 3 functions as a metabolite transporter

De Leonardis,

Ahmed,

Vozza

et al. 2023

FEBS Letters

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Since its discovery, a major debate about mitochondrial uncoupling protein 3 (UCP3) has been whether its metabolic actions result primarily from mitochondrial inner membrane proton transport, a process that decreases respiratory efficiency and ATP synthesis. However, UCP3 expression and activity are induced by conditions that would seem at odds with inefficient ‘uncoupled’ respiration, including fasting and exercise. Here, we demonstrate that the bacterially expressed human UCP3, reconstituted into liposomes, catalyses a strict exchange of aspartate, malate, sulphate and phosphate. The R282Q mutation abolishes the transport activity of the protein. Although the substrate specificity and inhibitor sensitivity of UCP3 display similarity with that of its close homolog UCP2, the two proteins significantly differ in their transport mode and kinetic constants.

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Energy substrate metabolism, mitochondrial structure and oxidative stress after cardiac ischemia-reperfusion in mice lacking UCP3

Cited by 7 publications

References 86 publications

Exploring cardiorenal syndrome in rats: insights from vibrational spectroscopy techniques

Exploring cardiorenal syndrome in rats: insights from vibrational spectroscopy techniques

Salvianolic acids and its potential for cardio-protection against myocardial ischemic reperfusion injury in diabetes

Human mitochondrial uncoupling protein 3 functions as a metabolite transporter

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