Energy response and modulation of AMPK pathway of the olive flounder Paralichthys olivaceus in low-temperature challenged

Lu, Yunliang; Nie, Miaomiao; Xiong, Yinghuai; Wang, Fang; Peng, Xiao; Wu, Zhihao; Liu, Ying

doi:10.1016/j.aquaculture.2017.11.031

Cited by 31 publications

(12 citation statements)

References 37 publications

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“…The PK activity and ATP concentration in the CT group were significantly higher than those in the CS group ( p < 0.05), which indicated that the flounder can enhance energy metabolism levels under low temperature to maintain body's metabolism and meet physiological activities. This was consistent with our previous study (Lu et al, ). D. rerio in the CT group could withstand low temperature environment by producing more ATP (Wang, Tan, Jiao, You, & Zhang, ), and metabolism and supply also were superior to the CS individuals, which could enhance the tolerance to low temperature in fish.…”

Section: Discussionsupporting

confidence: 94%

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Cold effect analysis and screening of SNPs associated with cold‐tolerance in the olive flounder Paralichthys olivaceus

You

et al. 2019

J Appl Ichthyol

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Olive flounder Paralichthys olivaceus is an important mariculture fish and cold stress limits to its growth and survival during winter. In the present study, the cold‐tolerant (CT) and cold‐sensitive (CS) flounder were defined after cold treatment (0.7 ± 0.05°C). Cold effects on histological and physiological and gene expression levels were analyzed. The correlation analyses between single nucleotide polymorphism locus (SNP) in hsp70, yb‐1 and hmgb1 and the trait of cold tolerance were performed. The results showed that flounder gill was obviously damaged after treatment and more serious lesions in some of the epithelial cell layers were observed in the CS group. ATP concentration and pyruvate kinase activity in the CT group were significantly higher than those in the CS group (p < 0.05). Expression levels of hsp70 and yb‐1 were both up‐regulated markedly after cold treatment (p < 0.05). Nine, twenty‐one and ten SNPs were screened from hsp70, yb‐1 and hmgb1 partial sequences, respectively. Among them, 3 SNPs in hsp70 and 2 SNPs in hmgb1 showed significant difference in the two groups. Allele G of the SNP 8 (locus 1797) in hsp70 was only observed in the CT group. In the SNP 7 (locus 725) of hmgb1, genotype TT and allele T, and genotype CC and allele C were associated with cold tolerance and cold sensitive, respectively. Furthermore, one haplotype (TTG) generated from SNPs of hsp70 gene among CT and CS individuals showed significant relationship with cold tolerance (p = 0.031). Two haplotypes (ATG) and (TCT) generated from SNPs of hmgb1 were significantly associated with resistance (p = 0.001) and sensitive (p = 0.002) in cold treatment, respectively. These SNPs, genotype, alleles and haplotypes of hsp70 and hmgb1 may be related to the cold resistance of flounder, and could be candidate markers potentially for further selective breeding.

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Section: Discussionsupporting

confidence: 94%

“…(Hu et al, ). Lu et al () found that the flounder increased ATP‐coupled ion transport and stabilized energy homeostasis to cope with cold stress. However, histological effect and SNP association with flounder cold tolerance are still unknown.…”

Section: Introductionmentioning

confidence: 99%

Cold effect analysis and screening of SNPs associated with cold‐tolerance in the olive flounder Paralichthys olivaceus

You

et al. 2019

J Appl Ichthyol

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“…The build‐up of AMP activates the cellular energy‐sensor, AMP‐activated protein kinase (AMPK), which in turn regulates local metabolic phenotypes (Figure 3d) (Hardie et al ., 2006). In fish, AMPK may be activated at relatively low ( e.g ., olive flounder, Paralichthys olivaceus , Temminck and Schlegel, 1846) (Lu et al ., 2018) or high temperatures ( e.g ., rainbow trout and coho salmon, O. mykiss , Walbaum, 179 2 , and Oncorhynchus kisutch , Walbaum, 1792) (Anttila et al ., 2013). Nonetheless, in goldfish ( Carassius auratus , Linnaeus 1758), AMPK activity was not associated with increased mitochondrial enzyme levels in response to cold acclimation, indicating some level of species specificity in AMPK action (Bremer et al ., 2015).…”

Section: Signalling Pathwaysmentioning

confidence: 99%

What do warming waters mean for fish physiology and fisheries?

Little

Loughland

Seebacher

2020

Journal of Fish Biology

127

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Environmental signals act primarily on physiological systems, which then influence higher-level functions such as movement patterns and population dynamics. Increases in average temperature and temperature variability associated with global climate change are likely to have strong effects on fish physiology and thereby on populations and fisheries. Here we review the principal mechanisms that transduce temperature signals and the physiological responses to those signals in fish. Temperature has a direct, thermodynamic effect on biochemical reaction rates. Nonetheless, plastic responses to longer-term thermal signals mean that fishes can modulate their acute thermal responses to compensate at least partially for thermodynamic effects. Energetics are particularly relevant for growth and movement, and therefore for fisheries, and temperature can have pronounced effects on energy metabolism. All energy (ATP) production is ultimately linked to mitochondria, and temperature has pronounced effects on mitochondrial efficiency and maximal capacities. Mitochondria are dependent on oxygen as the ultimate electron acceptor so that cardiovascular function and oxygen delivery link environmental inputs with energy metabolism.

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“…Recently, some studies have shown that the low utilization of carbohydrates by fish might be partly due to the poor postprandial supervision of certain energy metabolic sensors, which are closely involved in glucose metabolism ( Magnoni et al, 2012 ; Polakof et al, 2012 ; Condesieira and Soengas, 2017 ; Kamalam et al, 2017 ). Indeed, some studies have shown that the intermediary metabolism of fish could be regulated by these energy sensors that control intracellular glucose use ( Lu et al, 2018 ; Xu et al, 2018 ). In view of this, the molecular investigations of these energy sensors might promote our understanding of the carbohydrate utilization by fish.…”

Section: Introductionmentioning

confidence: 99%

Resveratrol Improves the Energy Sensing and Glycolipid Metabolism of Blunt Snout Bream Megalobrama amblycephala Fed High-Carbohydrate Diets by Activating the AMPK–SIRT1–PGC-1α Network

Shi

et al. 2018

Front. Physiol.

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This study investigated the effects of resveratrol on the growth performance, energy sensing, glycolipid metabolism and glucose and insulin load of blunt snout bream Megalobrama amblycephala fed high-carbohydrate diets. Fish (39.44 ± 0.06 g) were randomly fed three diets: a control diet (30% carbohydrate), a high-carbohydrate diet (HC, 41% carbohydrate), and the HC diet supplemented with 0.04% resveratrol (HCR) for 12 weeks. Fish fed the HC diet had significantly high values of nitrogen and energy retention efficiency, hepatosomatic index, intraperitoneal fat ratio, whole-body lipid content and intraperitoneal fat glycogen and lipid contents compared to the control group, but showed little difference with the HCR treatment. Liver and muscle lipid contents and plasma levels of glucose, glycated serum protein, advanced glycation end products and total cholesterol of fish fed the HC diet were significantly higher than those of the control group, whereas the opposite was found with resveratrol supplementation. Fish fed the HC diet obtained significantly low values of plasma insulin levels and hepatic adenosine monophosphate (AMP) contents and NAD+/NADH ratio compared to HCR treatment, but showed little difference with the control group. The opposite was found for hepatic adenosine triphosphate (ATP) contents and the ATP/AMP ratio. In addition, fish fed the HC diet showed significantly high transcriptions of glucose transporter 2 (GLUT2), glucose-6-phosphate dehydrogenase, glycogen synthase, fatty acid synthetase (FAS), acetyl-CoA carboxylase α (ACCα), peroxisome proliferator-activated receptor γ and PPARα compared to the control group, whereas the opposite was found for protein levels of AMP-activated protein kinase α (t-AMPKα), phosphorylated AMP-activated protein kinase α (p-AMPKα), sirtuin-1 (SIRT1), and p-AMPKα/t-AMPKα ratio as well as the transcriptions of AMPKα1, AMPKα2, SIRT1, PPARγ coactivator-1α (PGC-1α), phosphoenolpyruvate carboxykinase, fructose-1,6-bisphosphatase (FBPase), glucose-6-phosphatase, carnitine palmitoyl transferase I (CPT I) and acyl-CoA oxidase. Resveratrol supplementation significantly up-regulated the protein levels of t-AMPK, p-AMPK, and SIRT1, p-AMPK/t-AMPK ratio as well as the transcriptions of AMPKα1, AMPKα2, SIRT1, PGC-1α, GLUT2, FBPase, and CPT I compared to HC group, while the opposite was found for sterol regulatory element-binding protein-1, FAS and ACCα. Furthermore, resveratrol improved glucose and insulin tolerance of fish fed the HC diet after glucose and insulin load.

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Energy response and modulation of AMPK pathway of the olive flounder Paralichthys olivaceus in low-temperature challenged

Cited by 31 publications

References 37 publications

Cold effect analysis and screening of SNPs associated with cold‐tolerance in the olive flounder Paralichthys olivaceus

Cold effect analysis and screening of SNPs associated with cold‐tolerance in the olive flounder Paralichthys olivaceus

What do warming waters mean for fish physiology and fisheries?

Resveratrol Improves the Energy Sensing and Glycolipid Metabolism of Blunt Snout Bream Megalobrama amblycephala Fed High-Carbohydrate Diets by Activating the AMPK–SIRT1–PGC-1α Network

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