Energy-resolved mass spectrometry: a comparison of quadrupole cell and cone-voltage collision-induced dissociation

Abstract

Collision-induced dissociation (CID) can be effected in the interface region between atmospheric pressure ionization sources and single quadrupole mass analyzers. By varying the electric field in these cone-voltage CID experiments energy-resolved mass spectra can be obtained leading to breakdown graphs exploring the energy evolution of the fragmentation pathways. The breakdown graphs obtained from these cone-voltage CID studies are very comparable to those obtained by varying the collision energy in the quadrupole collision cell of a BEqQ mass spectrometer. The comparison has been made for the protonated peptides H-Leu-Gly-Gly-OH, H-Gly-Leu-Gly-OH, H-Gly-Gly-Leu-OH and Leu-enkephalin

Amide bond cleavage in deprotonated tripeptides: a newly discovered pathway to ″b2 ions

“…It is well known 19,20 that CID can be achieved in this region, so-called cone-voltage CID, and it has been clearly established [21][22][23] that the average energy imparted to the decomposing ions increases as the field in the interface region increases. Indeed, recent work [24][25][26][27] has shown that, by varying this field in steps, energy-resolved mass spectra [28][29][30] comparable to those obtained in variable low-energy CID in quadrupole cells can be obtained. The results of these cone-voltage CID experiments are presented in the following either as CID mass spectra at a set cone voltage or as breakdown graphs expressing the percent of total ion abundance as a function of the cone voltage, a measure of the field in the interface region.…”

Collision‐induced dissociation studies of caffeine in positive electrospray ionisation mass spectrometry using six deuterated isotopomers and one N1‐ethylated homologue

“…Breakdown graphs are established tools for the interpretation of CID fragmentation processes. [26,27] They display the percentage of a certain fragment population as a function of the cone voltage and allow the interpretation of the genesis and formation of the respective fragments. For caffeine no fragments could be observed up to 40 V but raising the cone voltage to about 70 V led to fragmentation.…”

Differentiation of Boc‐N‐protected α/β‐hybrid peptides containing β‐Caa‐L‐Ala‐β‐Caa‐OMe and β‐Caa‐L‐Ala‐β‐Caa‐NHMe at the C‐terminus by electrospray ionization tandem mass spectrometry

“…The breakdown graphs express the relative abundance of fragment ions as a function of the collision energy. [25,26] For example, in the case of tetrapeptide 1A which exhibits helix-turn, the intensity of b n-1 + ion starts increasing from 18 eV and remains constant up to 26 eV, whereas a similar trend is absent for the other peptide 1B (Fig. 3).…”