Energy Metabolism in Trypanosoma cruzi

Role of Δ1-Pyrroline-5-Carboxylate Dehydrogenase Supports Mitochondrial Metabolism and Host-Cell Invasion of Trypanosoma cruzi

“…In addition to the oxidation of ␥GS (catalyzed by TcP5CDH), glutamate can be enzymatically generated from the transamination of L-alanine or L-aspartate, the conversion of L-histidine, or the reductive amination of ␣-ketoglutarate (␣-KG). The latter step is reversible, where ␣-ketoglutarate acts as an intermediate metabolite of the Krebs cycle (109). These glutamate-related enzymes have been detected in both the mitochondria and the cytosol of T. cruzi (46,47).…”

Evaluation of the Anti-<i>Trypanosoma Cruzi</i> Effects of the Antipsychotic Drug Levomepromazine

“…This fact was previously observed by us and described as being probably strain dependant [21,22]. Starvation leading to nutritional stress occurs when the parasite changes its environment throughout its life cycle [26]. Particularly, it is well known that nutrient starvation occurs in the transit from the medium to the terminal portion of the insect gut [18].…”

JBP1 and JBP2 Proteins Are Fe2+/2-Oxoglutarate-dependent Dioxygenases Regulating Hydroxylation of Thymidine Residues in Trypanosome DNA

“…The requirement of 2-OG and inhibition by succinate in JBP1-stimulated TH activity indicate the 2-OG/succinate ratio within the parasite as a critical factor for J synthesis and may help explain the lack of base J in the procyclic T. brucei life stage (36). It is well characterized that procyclic T. brucei cells, in contrast to the long-slender bloodstream life stage that contains base J, produce high concentrations of succinate representing incompletely oxidized product of aerobic fermentation (37,38). The pathway(s) leading to lifecycle-specific production of succinate are not well understood, but the unique absence of a tricarboxylic acid cycle in bloodstream form T. brucei is presumably a key factor (39,40).…”