Energy deposition mechanisms and biochemical aspects of DNA strand breaks by ionizing radiation

Chatterjee, A.; Holley, W.R.

doi:10.1002/qua.560390508

Cited by 52 publications

(17 citation statements)

References 18 publications

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“…Such a LET-RBE (relative biological effectiveness) relationship is consistent with computational Monte-Carlo simulations Chatterjee and Holley 1991). However, recent measurements for mammalian cells yielded RBE values for DSB induction equal to or smaller than unity for neutron irradiation (Peak et al 1991) as well as for low-energy protons and slow c~-particles (Coquerelle et al 1987;Prise et al 1990).…”

Section: Introductionmentioning

confidence: 64%

Heavy ion-induced DNA double-strand breaks with yeast as a model system

Ikpeme

Löbrich

Akpa

et al. 1995

Radiat Environ Biophys

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Cells of diploid yeast, Saccharomyces cerevisiae, were exposed to a variety of energetic heavy ions (provided by the UNILAC facility at the Gesellschaft für Schwerionenforschung, GSI), 241Am alpha-particles and 80-keV x-rays after which they were assessed for DNA double-strand breaks (DSB) using either the neutral sedimentation or the pulsed-field gel electrophoresis (PFGE) technique. Both yielded comparable results. The DSB production cross-sections are compared with inactivation studies performed for the same cells under identical conditions. The measurements show that with lighter ions DSB induction cross-sections increase with linear energy transfer (LET), but the situation is less clear with the heavier ions. A close parallelism was found between DSB induction and cell inactivation in these yeast cells.

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Section: Introductionmentioning

confidence: 64%

Heavy ion-induced DNA double-strand breaks with yeast as a model system

Ikpeme

Löbrich

Akpa

et al. 1995

Radiat Environ Biophys

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“…For a particles, an increase in the yield of DSBs with linear energy transfer (LET) up to about 150 keV/pm has been found using some experimental systems (4)(5)(6), including mammalian cells in early studies (7,8). Such a behavior is also predicted by Monte Carlo simulations in computational studies (9,10). However, more recent experimental results using mammalian cells (11)(12)(13)(14)(15)(16)(17) have failed to show a substantial LET dependence for o particles, low-energy protons or neutrons, with a measured initial yield of DSBs nearly independent of LET.…”

Section: Introductionmentioning

confidence: 76%

“…Since the core is wide, the spatial distribution of ionizations within the core will be less dense compared to a low-energy ion of the same LET. No previous experimental results for induction of DSBs by such particles are available, but theoretical Monte Carlo calculations have been performed (10).…”

Section: Introductionmentioning

confidence: 99%

DNA Double-Strand Breaks Induced by High-Energy Neon and Iron Ions in Human Fibroblasts. I. Pulsed-Field Gel Electrophoresis Method

Cooper¹,

Rydberg²,

Löbrich³

1994

Radiation Research

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The relative effectiveness of high-energy neon and iron ions for the production of DNA double-strand breaks was measured in one transformed and one nontransformed human fibroblast cell line using pulsed-field gel electrophoresis. The DNA released from the gel plug (fraction of activity released: FAR) as well as the size distribution of the DNA entering the gel were used to compare the effects of the heavy-ion exposure with X-ray exposure. Both methods gave similar results, indicating similar distributions of breaks over megabase-pair distances for the heavy ions and the X rays. The relative biological effectiveness (RBE) compared to 225 kVp X rays of initially induced DNA double-strand breaks was found to be 0.85 for 425 MeV/u neon ions (LET 32 keV/microns) and 0.42-0.55 for 250-600 MeV/u iron ions (LET 190-350 keV/microns). Postirradiation incubation showed less efficient repair of breaks induced by the neon ions and the 600 MeV/u iron ions compared to X rays. Survival experiments demonstrated RBE values larger than one for cell killing by the heavy ions in parallel experiments (neon: RBE = 1.2, iron: RBE = 2.3-3.0, based on D10 values). It is concluded that either the initial yield of DNA double-strand breaks induced by the high-energy particles is lower than the yield for X rays, or the breaks induced by heavy ions are present in clusters that cannot be resolved with the technique used. These results are confirmed in the accompanying paper (M. Löbrich, B. Rydberg and P. Cooper, Radiat. Res. 139, 142-151, 1994).

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“…It is based on the evaluation of energy deposition at a nanometre scale by Monte Carlo codes reviewed in [11]. Such codes simulate electron and ion transport in biological or solid material [6,7,12,13,14,15] (Fig. 4).…”

Section: Radiation Tracksmentioning

confidence: 99%

General aspects of the cellular response to low- and high-LET radiation

2001

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Radiobiological studies have shown for some time that the effects of ionising radiation on cells are mainly explained by modification of the DNA. Numerous studies over the past 50 years have accumulated clear evidence of the cause-effect relationship between damage to DNA and the cytotoxic and mutagenic effects of ionising radiation. However, the path from irradiation of the cells to the induction of biological effects comprises several complex steps. The first step involves interactions between the radiation and the cellular environment. These consist of physical and chemical reactions which produce ions, excited molecules and radical species. Excitations and ionisations are complete in about 10(-15) s, and are followed by a chemical thermal equilibrium of the species produced within 10(-12) s. These species then diffuse from their site of production and provoke alterations to a variety of cellular components. This damage is detected by cellular surveillance systems, which in turn activate signalling cascades, gene transcription and enzyme recruitment, which participate in the cellular response. In most cases, cell cycle arrest occurs, allowing, according to the biological relevance of the DNA damage, either a process of DNA repair or programmed cell death (apoptosis). The accuracy of the DNA repair which is performed depends on the complexity of the DNA lesion and on the DNA repair machinery fidelity itself. Improper DNA repair can lead to mutation, chromosome aberration, genetic instability, oncogenic transformation and, ultimately, cell death.

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Energy deposition mechanisms and biochemical aspects of DNA strand breaks by ionizing radiation

Cited by 52 publications

References 18 publications

Heavy ion-induced DNA double-strand breaks with yeast as a model system

Heavy ion-induced DNA double-strand breaks with yeast as a model system

DNA Double-Strand Breaks Induced by High-Energy Neon and Iron Ions in Human Fibroblasts. I. Pulsed-Field Gel Electrophoresis Method

General aspects of the cellular response to low- and high-LET radiation

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