Energy‐dependent Processes in Chara Corallina: Absence of Light Stimulation When Only Photo‐system One Is Operative

Smith, Frank A.; Raven, John A.

doi:10.1111/j.1469-8137.1974.tb04600.x

Cited by 22 publications

(3 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus the fluxes and CO2 fixation rates at 25° C are similar to those previously found at 20-25°C where pH, illumination and composition of the medium were nearly the same as those used here (e.g. Smith & West, 1969;Smith, 1970;Smith & Raven, 1974;Lucas, 1975;Smith & Walker, 1978). The ^'SOf influx data of Robinson (1968) show similar temperature effects to those reported here for H2^^P04.…”

Section: Resultssupporting

confidence: 88%

“…The tracer influxes of Na* and Cl" are, at all temperatures, at least an order of magnitude greater than the maximum possible difference in net Na* and Cl" infiuxes which might occur between the various tem-perature treatments (Tables 1, 2 and 3). Tlie same may be true for K*; while the minimum tracer infiux of Rb* is only about twice the maximum possible difference in net K* fiux between the various temperature treatmetits, a consideration of previous results with C corallina (Hope, 1963;Findlay et ai, 1969;Doughty & Hope, 1973;Smith & Raven, 1974) suggests that the tracer influx of K* can be up to 5 times that of Rb*. Thus the difference in net fiux between different temperature treatments is, for all three ions, less than 10% of the tracer fiuxes.…”

Section: Resultsmentioning

confidence: 80%

See 1 more Smart Citation

Effect of temperature on ion content, ion fluxes and energy metabolism in Chara corallina

Raven

Smith

1978

Plant Cell & Environment

Self Cite

View full text Add to dashboard Cite

Effects of temperature on the ionic relations and energy metabolism of Chara corallina were investigated. Measurements were made of the ionic content, tracer ion fluxes, and photosynthetic and dark CO2 fixation in isolated cells, and of O2 exchange in photosynthesis and respiration in isolated shoot apices. The total intracellular concentration of K+, Na+ and Cl− was the same in cells held for 5 days in non‐growing medium at 15°C (the growth temperature) as in those held at 25°C or 5°C. The tracer influx in the light of all ions tested (Rb+, Na+, CH3NH3+, Cl− and H2PO4−) was lower at 5°C than at 15°C in experiments in which cells were subjected to 5°C for less than 24 h in toto. The influx at 25°C was greater than that at 15°C for H2PO−4, there was no difference between the two temperatures for Na+, while the influx at 25°C was less than that at 15°C for Cl−, Rb+ and CH3NH3+ For Cl− and H2PO−4 similar results were found in later experiments with cells grown at 20—23°C. Photosynthetic CO2 fixation and O2 evolution, and respiratory O2 uptake, are greater at 25°C, and lower at 5°C, than they are at the growth temperature of 15°C. In longer‐term pretreatments at the different temperatures, tracer Cl− influx at 15°C and particularly at 25°C were lower than in short‐term experiments, while the influx at 5°C was higher. It was concluded from these experiments, and from previous data on H+ free energy differences across the plasmalemma, that (1) the maintenance of internal ion concentrations involves a close balancing of influx and efflux of K+, Na+ and Cl− at all experimental temperatures; (2) the regulation of the tracer fluxes of the ions is kinetic rather than thermodynamic and (3) that the tracer fluxes at low temperatures are not restricted by the rate at which respiration or photosynthesis can supply energy to them.

show abstract

Section: Resultssupporting

confidence: 88%

Section: Resultsmentioning

confidence: 80%

Effect of temperature on ion content, ion fluxes and energy metabolism in Chara corallina

Raven

Smith

1978

Plant Cell & Environment

Self Cite

View full text Add to dashboard Cite

show abstract

“…Light usually enhances ion fluxes in algae (cf., 18,24), but in leaves of aquatic plants or leaf-slices of terrestrial higher plants, a variety of possible relations between light and metabolic ion absorption has been observed. In some cases light enhances ion absorption only under anaerobic conditions (15,23,25); in other cases light enhancement was observed under aerobic conditions as well (1, 14, 16,17,20,22).…”

Section: Resultsmentioning

confidence: 99%

Light Sensitivity of ²²Na, ⁸⁶Rb, and ⁴²K Absorption by Different Tissues of Bean Leaves

Jacoby¹

1975

Plant Physiol.

View full text Add to dashboard Cite

Autoradiographs of 22Na-loaded bean (Phaseolus vulgaris L.cv. 'Brittle Wax') leaf slices showed that most of the tracer was concentrated in vascular tissue. Rubidium-86 was uniformly distributed in slices that had been incubated in darkness; after incubation in the light many small dark spots appeared on the autoradiographs, apparently corresponding with the stomata.Autoradiographs of 42K-loaded slices showed a rather uniform distribution of the tracer, whether the slices had been incubated in light or in darkness.Leaf slices were incubated 1 hr in 0.1 mm chlorides of 22Na, 'K, or "6Rb at 30 C in light or darkness. The free space was exchanged with cold (0-1 C) 10 mm CaSO,. Detailed procedures have been described previously (25). For the autoradiographic location of absorbed ions, tracer-loaded leaf slices were rapidly frozen in liquid nitrogen and freeze-dried for 24 hr at -25 C. Dry slices were placed on AR-1O Kodak stripping films on microscope slides. RESULTSMetabolic energy for ion fluxes in green plant cells can apparently be supplied by photosynthesis. Light usually enhances ion fluxes in algae (cf., 18, 24), but in leaves of aquatic plants or leaf-slices of terrestrial higher plants, a variety of possible relations between light and metabolic ion absorption has been observed. In some cases light enhances ion absorption only under anaerobic conditions (15,23,25); in other cases light enhancement was observed under aerobic conditions as well (1,14,16,17,20,22). In barley-leaf slices, ion absorption was found to be stimulated only after aging the slices overnight (21).In unaged bean-leaf slices, the absorption of StRb and 42K was enhanced by light but, on the other hand, 2"Na absorption was light insensitive under aerobic conditions (9); the ATP content of these slices was also unaffected by illumination under aerobic conditions (25). Under anaerobic conditions, illumination restored the ATP content as well as the 2"Na absorption rate to those occurring under aerobic conditions. In view of these results, the light enhancement of "'Rb and 42K absorption under aerobic conditions by similar bean-leaf slices (9) cannot be explained simply by an effect of light on the ATP content of the cytoplasm of leaf cells.During the present work, we set out to investigate whether or not the light-enhanced s6Rb and 42K absorption in bean-leat slices and the light-insensitive absorption of 22Na occur in the same leaf tissues. MATERIALS AND METHODSBean plants (Phaseolus vulgaris L. cv. 'Brittle Wax') were grown at 25 C and with a 16-hr photoperiod as described in detail previously (11). Absorption experiments were carried out with leaf slices (10) aerated for 1 hr in 0.2 mM CaSO, before use. All incubation media also contained 0.2 mm CaSO4.

show abstract

Light-Driven Oxygen Consumption in the Water-Water Cycles and Photorespiration, and Light Stimulated Mitochondrial Respiration

Raven

Beardall

Quigg

2020

Photosynthesis in Algae: Biochemical and Physiological Mechanisms

View full text Add to dashboard Cite

Energy‐dependent Processes in Chara Corallina: Absence of Light Stimulation When Only Photo‐system One Is Operative

Cited by 22 publications

References 46 publications

Effect of temperature on ion content, ion fluxes and energy metabolism in Chara corallina

Effect of temperature on ion content, ion fluxes and energy metabolism in Chara corallina

Light Sensitivity of ²²Na, ⁸⁶Rb, and ⁴²K Absorption by Different Tissues of Bean Leaves

Light-Driven Oxygen Consumption in the Water-Water Cycles and Photorespiration, and Light Stimulated Mitochondrial Respiration

Contact Info

Product

Resources

About

Energy‐dependent Processes in Chara Corallina: Absence of Light Stimulation When Only Photo‐system One Is Operative

Cited by 22 publications

References 46 publications

Effect of temperature on ion content, ion fluxes and energy metabolism in Chara corallina

Effect of temperature on ion content, ion fluxes and energy metabolism in Chara corallina

Light Sensitivity of 22Na, 86Rb, and 42K Absorption by Different Tissues of Bean Leaves

Light-Driven Oxygen Consumption in the Water-Water Cycles and Photorespiration, and Light Stimulated Mitochondrial Respiration

Contact Info

Product

Resources

About

Light Sensitivity of ²²Na, ⁸⁶Rb, and ⁴²K Absorption by Different Tissues of Bean Leaves