Energy dependant plant stress acclimation

Dobrotă, Cristina

doi:10.1007/978-1-4020-6285-8_17

Cited by 7 publications

(6 citation statements)

References 30 publications

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“…It has been shown that ATP is one of the major mediators of stress transduction within the plant and that it is able to regulate the level of stress-related mRNA (Jeter et al 2004). The decrease in ATP can be explained since it is immediately mobilised by many cellular process (especially RNA and protein synthesis) as soon as a stress event occurs (Dobrota 2006) and decreases in AEC occur after cell stress, especially anoxia (Blokhina et al 2003). However, the adenylate nucleotide pool (ATP + ADP + AMP) remained at a steady-state level during our experiments (data not shown) suggesting that this energy decrease did not aVect cell metabolism in a profound manner.…”

Section: Discussionmentioning

confidence: 56%

High frequency (900 MHz) low amplitude (5 V m−1) electromagnetic field: a genuine environmental stimulus that affects transcription, translation, calcium and energy charge in tomato

Roux

Vian

Girard

et al. 2007

Planta

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Using an especially-designed facility, the Mode Stirred Reverberation Chamber, we exposed tomato plants (Lycopersicon esculentum Mill. VFN8) to low level (900 MHz, 5 V m(-1)) electromagnetic fields for a short period (10 min) and measured changes in abundance of three specific mRNA soon after exposure. Within minutes of electromagnetic stimulation, stress-related mRNA (calmodulin, calcium-dependent protein kinase and proteinase inhibitor) accumulated in a rapid, large and 3-phase manner typical of an environmental stress response. Accumulation of these transcripts into the polysomal RNA also took place (indicating that the encoded proteins were translated) but was delayed (indicating that newly-synthesized mRNA was not immediately recruited into polysomes). Transcript accumulation was maximal at normal Ca(2+) levels and was depressed at higher Ca(2+), especially for those encoding calcium-binding proteins. Removal of Ca(2+) (by addition of chelating agents or Ca(2+) channel blocker) led to total suppression of mRNA accumulation. Finally, 30 min after the electromagnetic treatment, ATP concentration and adenylate energy charge were transiently decreased, while transcript accumulation was totally prevented by application of the uncoupling reagent, CCCP. These responses occur very soon after exposure, strongly suggesting that they are the direct consequence of application of radio-frequency fields and their similarities to wound responses strongly suggests that this radiation is perceived by plants as an injurious stimulus.

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Section: Discussionmentioning

confidence: 56%

High frequency (900 MHz) low amplitude (5 V m−1) electromagnetic field: a genuine environmental stimulus that affects transcription, translation, calcium and energy charge in tomato

Roux

Vian

Girard

et al. 2007

Planta

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“…However, cellular PPi level remains unaffected during Pi-deficiency (Dancer et al, 1990). Thus, under P deficiency, cells bypass Pi and ATP-dependent enzymes in favour of PPi-dependent glycolytic enzymes, a process known as 'energetic flexibility' (Dobrota, 2006). In freshwater cyanobacteria, salinity severely reduces the cellular P-quota (Rai & Tiwari, 1999a;Rai & Sharma, 2006;Burut-Archanai et al, 2011, Tripathi et al, 2012, leading to depletion of cellular ATP (Avendaño & Valiente, 1994).…”

Section: Discussionmentioning

confidence: 99%

“…For example, Pi starvation causes dramatic decline in Pi, ADP and ATP levels; consequently, organisms must conserve ATP by bypassing ATP-requiring enzymatic pathways (Duff et al, 1989;Theodorou et al, 1992;Plaxton & Carswell, 1999;Uhde-Stone et al, 2003a;Dobrota, 2006). Earlier studies indicated also that Pi-independent enzymes catalysing alternative parallel reactions to adenylate-dependent glycolytic reactions operate under Pi-deficiency (Gauthier & Turpin, 1997;Plaxton & Carswell, 1999).…”

Section: Introductionmentioning

confidence: 99%

“…Photosynthetic efficiency decreases due to low ribulose-1.5-bisphosphate carboxylase/oxygenase (RuBisCO) activity, a reduced rate of ribulose-1.5-bisphosphate generation, impaired fluidity of thylakoid membranes (Rao, 1997;Brooks et al, 1998;Huang et al, 2005), and reduced phosphoglycerate, Calvin cycle intermediates (Dietz & Foyer, 1986), ATP/ADP ratio and NADPH synthesis (Dobrota, 2006). Non-nitrogen-fixing cyanobacteria fulfil their nitrogen demand mainly through nitrate assimilation catalysed by the enzymes nitrate reductase (NR) and nitrite reductase (NiR) (Herrero & Flores, 1997).…”

Section: Introductionmentioning

confidence: 99%

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Physiological, biochemical and molecular responses of the halophilic cyanobacteriumAphanothece halophyticato Pi-deficiency

Tripathi

Sharma

Kageyama

et al. 2013

European Journal of Phycology

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We studied the responses of a halophilic cyanobacterium Aphanothece halophytica at surplus (normal composition of growth medium containing 125 µM PO 4 3− ), sufficient (the minimum concentration supporting optimal growth, 22 µM PO 4 3− ) and deficient (no external supply of Pi) concentrations of inorganic phosphate (Pi). The cyanobacterium was able to grow well in Pi-deficient conditions until the end of incubation (14 days), though at a marginally reduced rate. The cellular P-quota in Pi-surplus cells at the end of incubation was 2.7 times that of their initial P-quota (0.75 µmol mg protein −1 ), and remained fairly high (0.442 µmol mg protein) even in Pi-deficient medium. However, cultures growing in Pi-sufficient medium (22 µM PO 4 3− ), upon transfer to Pi-deficient medium, exhibited a rapid decline in cellular P level. Furthermore, cells growing in Pi-surplus medium showed a rapid efflux of P into the external medium. Aphanothece halophytica exhibited a biphasic phosphate transport system involving both high-(K s 2.06 µM) and low-affinity (K s 17.85 µM) transporters. Cyanobacterial cells maintained a basal level (constitutively expressed and not affected by Pi availability) of alkaline phosphatase (APase) activity, which increased 5-7-fold under Pi-deficiency. Supplementation of phosphate to the medium caused gradual decline in the enzyme activity to the basal level. Pi-deficient cells showed an enhanced level of transcripts for PPi-dependent glycolytic enzymes. Though moderate, Pi-deficiency affected the respiration, photosynthetic rate and electron transport chain activity negatively. PS II activity was most sensitive to Pi-deficiency, followed by PSI and whole chain. Pi-replete A. halophytica cells showed a single high-affinity nitrate transport system. However, deficiency of Pi reduced the nitrate and nitrite reductase activities.

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“…It is likely that some traits require manipulation of more than one gene. Further scope for improvement of crop yield in drought-prone environment may lie in the improvement of efficient use of energy under stress through metabolic engineering as proposed by Dobrota (2006). The outcome of this type of genetic manipulation may be seen as a (Bahieldin et al 2005) and rice LEA3 (Xiao et al 2007) Heat shock proteins/chaperones OE HSP70-1 (Cho and Hong 2006) and BiP ( Alvim et al 126) Enzymes for the synthesis of osmoprotectants OE Mannitol-1-phosphate dehydrogenase (Abebe et al 2003) and 1 -pyrroline-5-carboxylate synthetase (Kavi Kishor et al 1995) Antioxidative enzymes for removal of reactive oxygen species…”

Section: Enhanced Drought Resistancementioning

confidence: 99%

Wild Relative and Transgenic Innovation for Enhancing Crop Adaptation to Warmer and Drier Climate

Gong

McIntyre

2011

Crop Adaptation to Climate Change

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Energy dependant plant stress acclimation

Cited by 7 publications

References 30 publications

High frequency (900 MHz) low amplitude (5 V m−1) electromagnetic field: a genuine environmental stimulus that affects transcription, translation, calcium and energy charge in tomato

High frequency (900 MHz) low amplitude (5 V m−1) electromagnetic field: a genuine environmental stimulus that affects transcription, translation, calcium and energy charge in tomato

Physiological, biochemical and molecular responses of the halophilic cyanobacteriumAphanothece halophyticato Pi-deficiency

Wild Relative and Transgenic Innovation for Enhancing Crop Adaptation to Warmer and Drier Climate

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