Energy conservation in the decarboxylation of dicarboxylic acids by fermenting bacteria

Dimroth, Peter; Schink, Bernhard

doi:10.1007/s002030050616

Cited by 125 publications

(96 citation statements)

References 58 publications

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“…It can be hypothesized that the decarboxylase in S. hydroxybenzoicum generates a Na + ion motive force as well. This would be equivalent to 1/3 mol ATP per mol substrate converted (Dimroth and Schink 1998). There is no evidence for any additional means of energy conservation in S. hydroxybenzoicum.…”

Section: Benzoyl-coa Metabolism In S Hydroxybenzoicummentioning

confidence: 96%

Initial steps in the fermentation of 3-hydroxybenzoate by Sporotomaculum hydroxybenzoicum

Müller

Schink

2000

Archives of Microbiology

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The anaerobic bacterium Sporotomaculum hydroxybenzoicum ferments 3-hydroxybenzoate to acetate, butyrate, and CO 2 . 3-Hydroxybenzoate was activated to 3-hydroxybenzoyl-CoA in a CoA-transferase reaction with acetyl-CoA or butyryl-CoA as CoA donors. 3-Hydroxybenzoyl-CoA was reductively dehydroxylated, forming benzoyl-CoA. This reaction was measured in cell-free extracts with cob(I)alamin as low-potential electron donor. No evidence was obtained that cob(I)alamin is the physiological electron donor; however, inhibitor studies indicated involvement of a strong nucleophile in the reaction. Benzoate was degraded by dense cell suspensions without a lag phase until an in situ ∆G′ value <-25 kJ mol -1 was reached. Benzoyl-CoA reductase was not detected. Enzyme activities for all reaction steps from glutaryl-CoA to butyryl-CoA, and ATP formation via acetate kinase were detected in cell-free extracts. GlutaconylCoA decarboxylase is likely to act as a primary sodium ion pump.

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Section: Benzoyl-coa Metabolism In S Hydroxybenzoicummentioning

confidence: 96%

Initial steps in the fermentation of 3-hydroxybenzoate by Sporotomaculum hydroxybenzoicum

Müller

Schink

2000

Archives of Microbiology

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“…strain CIB (29). In some organisms of limited energetic budget, such as fermenting bacteria, glutaryl-CoA is oxidized by a NAD-dependent glutaryl-CoA dehydrogenase and then decarboxylated by the action of a membrane-bound multicomponent glutaconylCoA decarboxylase, which is sodium dependent and couples decarboxylation with the translocation of a sodium ion across the membrane, leading to ATP synthesis (89). The conversion of glutaryl-CoA to crotonyl-CoA with the formation of ATP can be regarded as an additional means of energy conservation imposed by the strict energy constraints of syntrophic metabolism (107,328).…”

Section: Benzoate Catabolism: the Benzoyl-coa Degradation Pathwaymentioning

confidence: 99%

Anaerobic Catabolism of Aromatic Compounds: a Genetic and Genomic View

Carmona

Zamarro

Blázquez

et al. 2009

Microbiol Mol Biol Rev

388

381

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SUMMARY Aromatic compounds belong to one of the most widely distributed classes of organic compounds in nature, and a significant number of xenobiotics belong to this family of compounds. Since many habitats containing large amounts of aromatic compounds are often anoxic, the anaerobic catabolism of aromatic compounds by microorganisms becomes crucial in biogeochemical cycles and in the sustainable development of the biosphere. The mineralization of aromatic compounds by facultative or obligate anaerobic bacteria can be coupled to anaerobic respiration with a variety of electron acceptors as well as to fermentation and anoxygenic photosynthesis. Since the redox potential of the electron-accepting system dictates the degradative strategy, there is wide biochemical diversity among anaerobic aromatic degraders. However, the genetic determinants of all these processes and the mechanisms involved in their regulation are much less studied. This review focuses on the recent findings that standard molecular biology approaches together with new high-throughput technologies (e.g., genome sequencing, transcriptomics, proteomics, and metagenomics) have provided regarding the genetics, regulation, ecophysiology, and evolution of anaerobic aromatic degradation pathways. These studies revealed that the anaerobic catabolism of aromatic compounds is more diverse and widespread than previously thought, and the complex metabolic and stress programs associated with the use of aromatic compounds under anaerobic conditions are starting to be unraveled. Anaerobic biotransformation processes based on unprecedented enzymes and pathways with novel metabolic capabilities, as well as the design of novel regulatory circuits and catabolic networks of great biotechnological potential in synthetic biology, are now feasible to approach.

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“…The same cluster harbors two genes (Cf_2063 and Cf_2079) with coding homology to several thiamine pyrophosphate-requiring enzymes, including oxalyl-CoA decarboxylases. In the anaerobic bacterium Oxalobacter formigenes, which belongs to the same family as Collimonas, the net activity of these activities (that is oxalate in, formate out) generates a proton gradient from which the bacterium derives energy (Anantharam et al, 1989;Dimroth and Schink, 1998). Downstream of Cf_2063 are four genes (Cf_2064-2067) that code for the a-, b-, g-and d-subunits of an NAD-dependent formate dehydrogenase (Oh and Bowien, 1998).…”

Section: Carbon and Energy Metabolismmentioning

confidence: 99%

Dual transcriptional profiling of a bacterial/fungal confrontation: Collimonas fungivorans versus Aspergillus niger

et al. 2011

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Interactions between bacteria and fungi cover a wide range of incentives, mechanisms and outcomes. The genus Collimonas consists of soil bacteria that are known for their antifungal activity and ability to grow at the expense of living fungi. In non-contact confrontation assays with the fungus Aspergillus niger, Collimonas fungivorans showed accumulation of biomass concomitant with inhibition of hyphal spread. Through microarray analysis of bacterial and fungal mRNA from the confrontation arena, we gained new insights into the mechanisms underlying the fungistatic effect and mycophagous phenotype of collimonads. Collimonas responded to the fungus by activating genes for the utilization of fungal-derived compounds and for production of a putative antifungal compound. In A. niger, differentially expressed genes included those involved in lipid and cell wall metabolism and cell defense, which correlated well with the hyphal deformations that were observed microscopically. Transcriptional profiles revealed distress in both partners: downregulation of ribosomal proteins and upregulation of mobile genetic elements in the bacteria and expression of endoplasmic reticulum stress and conidia-related genes in the fungus. Both partners experienced nitrogen shortage in each other's presence. Overall, our results indicate that the Collimonas/ Aspergillus interaction is a complex interplay between trophism, antibiosis and competition for nutrients.

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Energy conservation in the decarboxylation of dicarboxylic acids by fermenting bacteria

Cited by 125 publications

References 58 publications

Initial steps in the fermentation of 3-hydroxybenzoate by Sporotomaculum hydroxybenzoicum

Initial steps in the fermentation of 3-hydroxybenzoate by Sporotomaculum hydroxybenzoicum

Anaerobic Catabolism of Aromatic Compounds: a Genetic and Genomic View

Dual transcriptional profiling of a bacterial/fungal confrontation: Collimonas fungivorans versus Aspergillus niger

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