Energy conservation by succinate decarboxylation in Veillonella parvula

Denger, Karin; Schink, Bernhard

doi:10.1099/00221287-138-5-967

Cited by 21 publications

(17 citation statements)

References 22 publications

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“…For example, the anaerobe Veillonella parvula can generate ATP in a process that is coupled to the decarboxylation of succinate (Denger & Schink, 1992;Janssen, 1992). Since succinate supplementation partially restores growth of FRD-deficient B. fragilis, it is likely that this bacterium also uses succinate for the conservation of metabolic energy.…”

Section: Discussionmentioning

confidence: 99%

The essential role of fumarate reductase in haem-dependent growth stimulation of Bacteroides fragilis

Baughn

Malamy

2003

Microbiology

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Haem is required for optimal growth of the bacterial anaerobe Bacteroides fragilis. Previous studies have shown that growth in the presence of haem is coincident with increased yields of ATP from glucose, expression of b-type cytochromes and expression of fumarate reductase activity. This paper describes the identification of the genes that encode the cytochrome, iron-sulfur cluster protein and flavoprotein of the B. fragilis fumarate reductase. These genes, frdC, frdA and frdB, respectively, are organized in an operon. Nonpolar, in-frame deletions of frdC and frdB were constructed in the B. fragilis chromosome. These mutant strains had no detectable fumarate reductase or succinate dehydrogenase activity. In addition, the frd mutant strains showed a threefold increase in generation time, relative to the wild-type strain. Growth of these mutant strains was fully restored to the wild-type rate by the introduction of a B. fragilis replicon containing the entire frd operon. Growth of the frd mutant strains was partially restored by supplementing the growth medium with succinate, indicating that the frd gene products function as a fumarate reductase. During growth on glucose, the frd mutant strains showed a threefold decrease in cell mass yield, relative to the wild-type strain. These data indicate that fumarate reductase is important for both energy metabolism and succinate biosynthesis in B. fragilis.

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Section: Discussionmentioning

confidence: 99%

The essential role of fumarate reductase in haem-dependent growth stimulation of Bacteroides fragilis

Baughn

Malamy

2003

Microbiology

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“…This bacterium carboxylates pyruvate to oxaloacetate not by a transcarboxylase reaction with methylmalonyl-CoA as carboxylic group donor, but by an ATP-consuming pyruvate carboxylase [7]. Decarboxylation of methylmalonyl-CoA to propionyl-CoA is coupled to the establishment of a transmembrane sodium ion gradient [23,24] which can conserve the equivalent of 1/3 ATP unit per reaction [25].…”

Section: Discussionmentioning

confidence: 99%

Energetics and kinetics of lactate fermentation to acetate and propionate via methylmalonyl-CoA or acrylyl-CoA

Seeliger

2002

FEMS Microbiology Letters

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Fermentation balances and growth yields were determined with various bacteria fermenting lactate to acetate plus propionate either via methylmalonyl-CoA or via acrylyl-CoA. All strains fermented lactate to acetate plus propionate at approximately a 1:2 ratio. Growth yields of Propionibacterium freudenreichii were more than twice as high as those of Clostridium homopropionicum or Veillonella parvula. Hydrogen was formed as a side product to a significant extent only by V. parvula and Pelobacter propionicus; the latter formed hydrogen preferentially when using ethanol as substrate. Acrylyl-CoA reductase of C. homopropionicum and Clostridium neopropionicum was found nearly exclusively in the cytoplasm thus confirming that this reduction step is unlikely to be involved in energy conservation. C. homopropionicum exhibited higher K S and higher W max values, as well as higher specific substrate turnover rates than P. freudenreichii. The results allow us to conclude that C. homopropionicum using the acrylyl-CoA pathway with low growth yield obtains its specific competitive advantage compared to P. freudenreichii not through higher substrate affinity or metabolic shift toward enhanced acetate-plus-hydrogen formation but through faster specific substrate turnover. ß

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“…threeand four-carbon organic acids, as energy and carbon sources via the methylmalonyl-CoA pathway (Delwiche et al, 1985;Denger & Schink, 1992;Seeliger et al, 2002). In the gastrointestinal tract, strains of the genus Veillonella thereby constitute an essential link with indigenous lactic acid bacteria in a natural microbial food-chain (Marounek & Bartos, 1987;Pacheco Delahaye et al, 1994;Kolenbrander, 2006).…”

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confidence: 99%

Veillonella magna sp. nov., isolated from the jejunal mucosa of a healthy pig, and emended description of Veillonella ratti

Kraatz

Taras

2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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Veillonella magna sp. nov., isolated from the jejunal mucosa of a healthy pig, and emended description of Veillonella ratti A bacterium, designated strain lac18 T , was isolated in pure culture from the mucosal jejunum of a healthy pig, using a medium selective for anaerobic lactic acid bacteria and containing porcine gastric mucin as the main carbon and nitrogen source. Cells of this strain were coccus-shaped, arranged singly or in pairs and were Gram-stain-negative, oxidase-negative, non-spore-forming, anaerobic and microaerotolerant. An analysis based on 16S rRNA gene sequences indicated that strain lac18 T should be assigned to the genus Veillonella, class Clostridia, phylum Firmicutes. 16S rRNA and dnaK gene sequence-based phylogenetic analyses both indicated that the most closely related species were Veillonella ratti ATCC 17746 T (similarities of 96.6 and 84.5 %, respectively) and Veillonella criceti ATCC 17747 T (similarities of 96.6 and 83.4 %, respectively). The results of DNA-DNA hybridizations between strain lac18 T and these Veillonella species and the type species of the genus, Veillonella parvula DSM 2008 T , confirmed the genotypic distinctness of the novel isolate. Data from phenotypic studies also served to differentiate strain lac18 T from related strains. Therefore strain lac18 T represents a novel species of the genus Veillonella, for which the name Veillonella magna sp. nov. is proposed. The type strain is lac18 T

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Energy conservation by succinate decarboxylation in Veillonella parvula

Cited by 21 publications

References 22 publications

The essential role of fumarate reductase in haem-dependent growth stimulation of Bacteroides fragilis

The essential role of fumarate reductase in haem-dependent growth stimulation of Bacteroides fragilis

Energetics and kinetics of lactate fermentation to acetate and propionate via methylmalonyl-CoA or acrylyl-CoA

Veillonella magna sp. nov., isolated from the jejunal mucosa of a healthy pig, and emended description of Veillonella ratti

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