Energetics of Fish Spermatozoa

Kholodnyy, Vitaliy; Boryshpolets, Sergii; Dzyuba, Borys; Cosson, Jacky

doi:10.1007/978-981-15-4025-7_4

Cited by 3 publications

(2 citation statements)

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“…Thus, the results in the present study reinforce the importance of the interaction between ROS and Ca 2+ in the mitochondria for sperm physiology. Furthermore, these results indicate that cryodamage affected sperm plasma membrane and the calcium‐binding proteins present in it, which are important in the regulation of sperm motility (Cosson, 2019; Kholodnyy et al, 2020; Yoshida & Asturiano, 2020).…”

Section: Discussionmentioning

confidence: 95%

Sperm cryopreservation of Prochilodus lineatus throughout the same reproductive season

et al. 2021

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The aim of this study was to determine the effect of seasonality on post‐thaw sperm quality in Prochilodus lineatus. Sperm from 43 males was cryopreserved throughout the reproductive season (November–March). Fresh sperm quality, concentration, volume, seminal plasma pH, osmolality and ion concentration were analysed. Post‐thaw sperm was analysed for its motility rate, velocities, beat‐cross frequency, cell morphology, oxidative stress and fertilization capacity. Prochilodus lineatus fresh sperm yielded high‐quality during the reproductive period (motility >80%). Post‐thaw sperm quality changed throughout the season, with higher motility (63.2%–72.3%) and curvilinear velocity (VCL) (55.9–59.2 µm/s) from December to March. Sperm concentration was negatively correlated with post‐thaw sperm motility and VCL, and positively correlated with seminal plasma Ca2+ concentration, catalase activity (CAT) and reactive oxygen species (ROS). Seminal plasma Ca2+ was positively correlated with ROS and negatively correlated with post‐thaw sperm motility. Post‐thaw sperm CAT and fertility correlated positively. Although post‐thaw sperm quality was reduced in November, the increased CAT was efficient in maintaining its fertilization capacity. In order to face seasonal influence, the optimal period to cryopreserve P. lineatus spermatozoa is from December to March, when sperm exhibits characteristics which make spermatozoa more prone to resist cryodamage.

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Section: Discussionmentioning

confidence: 95%

Sperm cryopreservation of Prochilodus lineatus throughout the same reproductive season

et al. 2021

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“…Mitochondria are the organelles responsible for providing energy to fish sperm in the form of ATP (Kholodnyy et al, 2020). Therefore, the mitochondrial activity of gametes is essential for reproductive success (Figueroa et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Development and application of low-cost and environment-friendly techniques for fish sperm cryopreservation

de Souza França

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Fish sperm cryopreservation is a technique that can increase the reproduction in captive efficiency of freshwater and marine fishes. The technique is applied in environmental conservation programs and aquaculture. Over the last few decades, protocols for sperm cryopreservation from many fishes have been established. However, the researchers' main focus was successfully freezing and thawing sperm, neglecting the period in which the gametes remain in contact with the cryoprotective solution until fertilization. Exposure of sperm to cryoprotectant solutions after thawing can be harmful to the quality of the gametes since they can be toxic.The vast majority of the established protocols use ultra-resistant plastic containers to store sperm during the cryopreservation process. These containers usually are not reused, generating highly polluting waste for the environment. Furthermore, in some countries, the containers usually used are sold by a few industries, which makes acquisition difficult and increases the product's price. Thus, the main objective of the thesis was to create and test low-cost methodologies that enhance the use of fish post-thaw sperm and make the sperm cryopreservation process more environmentally friendly.The experiments in the Chapters 1 and 2 were developed in Brazil. We used the South American silver catfish Rhamdia quelen, a species considered an experimental model for native South American fishes. In vitro fertilization and sperm cryopreservation protocols for the species were already established; thus, we based our work on these protocols to apply innovations.In Chapter 1, we tested the use of post-thawing dilution to reduce the toxicity of the cryoprotectant solution. This technique is commonly used in mammalian sperm cryopreservation protocols but has never before been applied to post-thaw sperm of South American fishes. South American silver catfish post-thaw sperm samples were diluted in a saline extender (1.1% NaCl -325 mOsm kg -1 ; pH 7.6). The post-thaw sperm diluted samples showed higher velocities, straightness, progression, and flagellar beat frequency than SUMMARY 2 the cells of undiluted samples (control). The post-thawing dilution also provided higher fertilization and hatching rates than the control group. Thus, the post-thawing sperm dilution proved to be a simple, cheap, and efficient methodology that should be included in the silver catfish sperm cryopreservation protocol.

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