Energetics and Dynamics of Fragmentation of Protonated Leucine Enkephalin from Time- and Energy-Resolved Surface-Induced Dissociation Studies

Laskin, Julia

doi:10.1021/jp057229r

Cited by 54 publications

(127 citation statements)

References 59 publications

(153 reference statements)

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“…Internal fragments GF-28, GF, GGF, and FYG are observed as well. Laskin 24 found a strong time dependence of a 4 -NH 3 and FYG and concludes that these fragments are associated with the cyclic rearrangements previously mentioned. This cyclic a 4 rearrangement has already been invoked earlier by Vachet and co-workers.…”

Section: Discussionmentioning

confidence: 62%

“…When we assume that the excitation of one of the peptide bonds is followed by IVR, we can directly compare our results to the SID studies by Laskin. 24 For 50 eV protonated leu-enk collisions with a self-assembled alkane thiolate monolayer, a maximum of about 17% of the collision energy is transferred into the peptide 38 implying about 8.5 eV of internal energy-about the same as deposited by 8 or 9 eV photoexciation here. The SID spectrum, qualitatively similar to our photoexcitation data [see Fig.…”

Section: Discussionmentioning

confidence: 78%

“…35 The fragments b 3 , GF, and 120 (F) show no time dependence and are formed following entropically favored pathways. 24 Despite the good agreement in fragments observed, the fragment yields are very different: For SID, a 4 = 397 and b 4 = 425 are the strongest fragments whereas (F) and (Y) are weak. The opposite is true for our 8 eV photon data [ Fig.…”

Section: Discussionmentioning

confidence: 94%

“…Leu-enk has the additional advantage that it has already been investigated by a whole arsenal of mass-spectrometric tools. The obtained fragmentation pattern and yields will be interpreted in the context of existing data on leu-enk surface-induced dissociation (SID), 24 blackbody infrared radiative dissociation (BIRD), 25 laser-induced dissociation (LID), 26 multiphotoninduced 27 and collision-induced (CID) 28 mass spectra. The influence of the electronic structure of the peptide and its constituent amino acids on the dissociation and ionization will be discussed.…”

Section: Introductionmentioning

confidence: 94%

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Photodissociation of protonated leucine-enkephalin in the VUV range of 8–40 eV

Bari

González-Magaña

Reitsma

et al. 2011

The Journal of Chemical Physics

116

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Until now, photodissociation studies on free complex protonated peptides were limited to the UV wavelength range accessible by intense lasers. We have studied photodissociation of gas-phase protonated leucine-enkephalin cations for vacuum ultraviolet (VUV) photons energies ranging from 8 to 40 eV. We report time-of-flight mass spectra of the photofragments and various photofragmentyields as a function of photon energy. For sub-ionization energies our results are in line with existing studies on UV photodissociation of leucine-enkephalin. For photon energies exceeding 10 eV we could identify a new dissociation scheme in which photoabsorption leads to a fast loss of the tyrosine side chain. This loss process leads to the formation of a residual peptide that is remarkably cold internally.

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Section: Discussionmentioning

confidence: 62%

Section: Discussionmentioning

confidence: 78%

Section: Discussionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 94%

See 2 more Smart Citations

Photodissociation of protonated leucine-enkephalin in the VUV range of 8–40 eV

Bari

González-Magaña

Reitsma

et al. 2011

The Journal of Chemical Physics

116

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“…Time-resolved SID studies have been conducted in an FT-ICR by varying the trapping time following peptide ion-surface collision [103][104][105][106]. These studies revealed the time dependence of peptide fragmentation when the observation time frame ranged from 1 ms to several seconds.…”

Section: Kinetics Of Peptide Fragmentationmentioning

confidence: 99%

Surface-induced dissociation of small molecules, peptides, and non-covalent protein complexes

Wysocki

Joyce

Jones

et al. 2008

J. Am. Soc. Mass Spectrom.

130

138

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This article provides a perspective on collisions of ions with surfaces, including surfaceinduced dissociation (SID) and reactive ion scattering spectrometry (RISS). The content is organized into sections on surface-induced dissociation of small ions, surface characterization of organic thin films by collision of well-characterized ions into surfaces, the use of SID to probe peptide fragmentation, and the dissociation of large non-covalent complexes by SID. Examples are given from the literature with a focus on experiments from the authors' laboratory. The article is not a comprehensive review but is designed to provide the reader with an overview of the types of results possible by collisions of ions into surfaces. (J Am Soc Mass Spectrom 2008, 19, 190 -208) © 2008 American Society for Mass Spectrometry T andem mass spectrometry (MS/MS) is an essential tool for elucidating ion structure. The MS/MS experiment involves mass selection of a precursor ion followed by ion activation and subsequent dissociation. The ion activation step is commonly accomplished via collision-induced dissociation (CID) in which the initial kinetic energy of a projectile ion is converted into internal energy through inelastic collisions with a neutral gas. Several alternative activation methods have been used in tandem mass spectrometry, one of which is surfaceinduced dissociation (SID). SID is analogous to CID, except that a surface replaces the neutral gas as the collision target. A typical ion-surface collision event is illustrated in Figure 1.The incorporation of a surface into a mass spectrometer for ion activation was pioneered in the laboratory of R. Graham Cooks in the mid-1970s and early 1980s [1][2][3]. Since that time, collisions of lowenergy (eV) organic ions with surfaces within the tandem mass spectrometer have been valuable for analyzing surface composition, characterizing reactions between organic projectile ions and surface adsorbates, chemically modifying surfaces, and determining projectile ion structure. A major motivation for development of SID is that energy transfer to ionic projectiles can be improved by increasing the mass of the collision target. The total available energy for transfer into the internal modes of the projectile ion is defined by the center-of-mass energy (E COM ) described by eq 1:where E LAB is the laboratory collision energy and M ION and M N are the masses of the projectile ion and neutral, respectively. Energy transfer in CID is limited by the mass of the collision partner, typically inert gases such as helium, argon, or xenon. In SID, if one assumes that the surface is an infinitely large collision partner, E COM becomes independent of mass and approaches the laboratory collision energy. The assumption that the entire surface can be viewed as the collision partner is not always valid, however, and there are instances where the mass of the terminal groups on the surface influence the amount of energy transfer [4,5]. Nonetheless, the use of a massive surface target should, in theory, provide ...

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Candida Cholecystitis in a Diabetic Patient on Chronic Peritoneal Dialysis

Weng

Chang

Lin

et al. 2008

Dialysis & Transplantation

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To examine the antiparkinsonian effects of blocking glycineB receptors, we designed a pilot study testing the potent and selective antagonist, PAMQX, in 1‐methyl‐4‐phenyl‐1,2,3,6‐tetrahydropyridine‐treated primates. PAMQX had no intrinsic effects but markedly potentiated the antiparkinsonian action of levodopa. In a dose‐dependent fashion, coadministration of the glycineB antagonist with levodopa extended the response duration by nearly 60%. It is noteworthy that PAMQX, within a considerable dose range, did not cause ataxia or other side effects. These data indicate that blocking N‐methyl‐D‐aspartate receptors selectively to manipulate dopaminergic‐mediated motor responses may be produced effectively by glycineB antagonists. Ann Neurol 2004

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Energetics and Dynamics of Fragmentation of Protonated Leucine Enkephalin from Time- and Energy-Resolved Surface-Induced Dissociation Studies

Cited by 54 publications

References 59 publications

Photodissociation of protonated leucine-enkephalin in the VUV range of 8–40 eV

Photodissociation of protonated leucine-enkephalin in the VUV range of 8–40 eV

Surface-induced dissociation of small molecules, peptides, and non-covalent protein complexes

Candida Cholecystitis in a Diabetic Patient on Chronic Peritoneal Dialysis

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