[1] Theoretical model calculations recently predicted the existence of mesospheric irregularities which assist the initiation of sprites. Here we report the experimental detection of a 3-19 km 3 large mesospheric irregularity at 80-85 km height which is illuminated by the electromagnetic field of an intense positive cloud-to-ground lightning discharge. While the lightning discharge causes a prompt group of four sprites above the lightning discharge, the mesospheric irregularity is found at a horizontal distance at least 15-20 km away from the sprite group and it rebrightens 40-60 ms after the sprite group occurrence. This rebrightening is driven by a local quasi-static electric field enhancement with a charge moment 4-20 C km which causes the irregularity to develop a downward descending luminous column from 75-85 km height. The quasi-static electric field enhancement is caused by the reorganization of residual charge inside the thundercloud during a high-level activity of intracloud discharges with 10-20 pulses per ms. Such mesospheric irregularities might have an effect on the wave propagation of 100 kHz radio waves which are used for atomic time transfer and marine navigation. Citation: Füllekrug, M., A. Mezentsev, S. Soula, O. van der Velde, and A. Evans (2013), Illumination of mesospheric irregularity by lightning discharge, Geophys. Res. Lett., 40,[6411][6412][6413][6414][6415][6416]