Enduring toxicity of transgenic Anabaena PCC 7120 expressing mosquito larvicidal genes from Bacillus thuringiensis ssp. israelensis†

Manasherob, Robert; Otieno-Ayayo, Z. Ngalo; Ben‐Dov, Eitan; Miaskovsky, Rina; Boussiba, Sammy; Zaritsky, Arieh

doi:10.1046/j.1462-2920.2003.00503.x

Cited by 13 publications

(11 citation statements)

References 18 publications

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“…Other Recipient Strains Apart from the strains above, other recipients such as cyanobacteria (Chungjatupornchai 1990;Lluisma et al 2001a;Lu et al 1999b;Manasherob et al 2002aManasherob et al , 2003bWu et al 1997a), Rhizobium (Nambiar et al 1990), and even the baculoviruses Crook 1993, 1998) have also been used as recipients for the expression of various ICP genes.…”

Section: Recipient Strainsmentioning

confidence: 99%

“…This organism, which serves as a food source for mosquito larvae and multiplies in their breeding sites, may solve the environment-imposed limitations of Bti as a biological control agent for mosquitoes. Under simulated field conditions, the transgenic Anabaena PCC 7120 expressing the Bti genes had greater mosquito larvicidal activity than that of Bti primary powder (fun 89C06D) or wettable powder (WP) (Bactimos products) when either was mixed with silt or exposed to sunlight outdoors (Manasherob et al 2003a). …”

Section: Constructing the Engineered Bacteria With Entomotoxic And Nimentioning

confidence: 99%

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Genetically Modified Bacillus thuringiensis Biopesticides

Zhou

2012

Bacillus Thuringiensis Biotechnology

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The reduction in crop production that results from damages of pests and pathogenic microbes has been the major prohibitive factor restricting the further development of agricultural production worldwide. In the long-term practice of controlling agricultural pests, multiple pest management, including biological approaches, are generally recognized as possible solutions to control pest infestation effectively. Bacterial pesticides, which constitute a series of leading biopesticidal products made from various naturally occurring or genetically modified insecticidal bacteria, have attracted increasing attention as a specific means of controlling agricultural and forestry pests.

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Section: Recipient Strainsmentioning

confidence: 99%

Section: Constructing the Engineered Bacteria With Entomotoxic And Nimentioning

confidence: 99%

Genetically Modified Bacillus thuringiensis Biopesticides

Zhou

2012

Bacillus Thuringiensis Biotechnology

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“…They were about seven-fold more effective than a commercial preparation of Bti itself. 18 One of our future plans, adding the last major Cry gene cry4Ba to this battery, would improve this bio-control agent.…”

Section: Cyanobacteria To Deliver Bti Toxins Against Mosquitoesmentioning

confidence: 99%

Transgenic organisms expressing genes fromBacillus thuringiensisto combat insect pests

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Bti is highly efficient and specific against mosquito and black fly larvae. 3 Most importantly, no resistance has been observed in nature after about 30 years of extensive use worldwide. 4 Different activities and modes of action of its four major toxins form a lethal combination against larvae of all mosquito species tested. 5 Resistance is not selected due to synergy among Bti components, mostly the lowtoxic, non-specific Cyt1Aa. High synergy levels affected by Cyt1Aa were observed by Crickmore 6 and Wirth, 7 the latter also demonstrated that Cyt1Aa prevented selection of resistant mosquitoes. 8The question raised was whether a combination of antiLepidopteran toxins with Cyt1Aa imitates this rare advantage of Bti. To partially answer this question, two genes were cloned for expression in Escherichia coli, cry1Ac (from Bt ssp. kurstaki) and cry1Ca (from Bt ssp. aizawai), with and without cyt1Aa, and tested against three pests, Helicoverpa armigera, Pectinophora gossypiella and Spodoptera littoralis.9 Co-expression of all three genes, and with p20 encoding an accessory protein (for reasons beyond Various subspecies (ssp.) of Bacillus thuringiensis (Bt) are considered the best agents known so far to control insects, being highly specific and safe, easily mass produced and with long shelf life.1 The para-crystalline body that is produced during sporulation in the exosporium includes polypeptides named d-endotoxins, each killing a specific set of insects. The different entomopathogenic toxins of various Bt ssp. can be manipulated genetically in an educated way to construct more efficient transgenic bacteria or plants that express combinations of toxin genes to control pests.2 Joint research projects in our respective laboratories during the last decade demonstrate what can be done by implementing certain ideas using molecular biology with Bt ssp. israelensis (Bti) as a model system. Here, we describe our progress achieved with Gram-negative bacterial species, including cyanobacteria, and some preliminary experiments to form transgenic plants, mainly to control mosquitoes (Diptera), but also a particular Lepidopteran and Coleopteran pest species. In addition, a system is described by which environment-damaging genes can be removed from the recombinants thus alleviating procedures for obtaining permits to release them in nature.

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“…Although there is low risk of resistance being developed to Bti [10], there are several disadvantages to using Bti as a control agent [11,12]. These include its low persistence in the field due to inactivation by UV, ingestion of Bti by other aquatic organisms, and the settling of Bti from the mosquito larval feeding zone [11-13]. One strategy to overcome some of the disadvantages of Bti is to clone the cry genes of Bti into aquatic microorganisms that: (1) are not toxic to other organisms, (2) inhabit and persist in the larval feeding zone, (3) are used by mosquito larvae as a food source, (4) express Cry proteins at levels that are mosquito larvicidal, and (5) have cell walls that reduce inactivation of the Cry proteins by UV [13-15].…”

Section: Introductionmentioning

confidence: 99%

“…The Bti genes are integrated into the chromosome of PCC 7120, resulting in a stable recombinant strain [16]. Laboratory bioassays have shown that the resultant recombinant strain, PCC 7120#11, is a very effective larvicidal agent against Aedes aegypti [13,15,16]. …”

Section: Introductionmentioning

confidence: 99%

The susceptibility of five African Anopheles species to Anabaena PCC 7120 expressing Bacillus thuringiensis subsp. israelensis mosquitocidal cry genes

Ketseoglou

Bouwer

2012

Parasites Vectors

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BackgroundMalaria, one of the leading causes of death in Africa, is transmitted by the bite of an infected female Anopheles mosquito. Problems associated with the development of resistance to chemical insecticides and concerns about the non-target effects and persistence of chemical insecticides have prompted the development of environmentally friendly mosquito control agents. The aim of this study was to evaluate the larvicidal activity of a genetically engineered cyanobacterium, Anabaena PCC 7120#11, against five African Anopheles species in laboratory bioassays.FindingsThere were significant differences in the susceptibility of the anopheline species to PCC 7120#11. The ranking of the larvicidal activity of PCC 7120#11 against species in the An. gambiae complex was: An. merus

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Enduring toxicity of transgenic Anabaena PCC 7120 expressing mosquito larvicidal genes from Bacillus thuringiensis ssp. israelensis^†

Cited by 13 publications

References 18 publications

Genetically Modified Bacillus thuringiensis Biopesticides

Genetically Modified Bacillus thuringiensis Biopesticides

Transgenic organisms expressing genes fromBacillus thuringiensisto combat insect pests

The susceptibility of five African Anopheles species to Anabaena PCC 7120 expressing Bacillus thuringiensis subsp. israelensis mosquitocidal cry genes

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