Endurance training affects myosin heavy chain phenotype in regenerating fast-twitch muscle

Bigard, Xavier; Janmot, Chantal; Merino, D.; Lienhard, F.; Guezennec, Y.; d’Albis, Anne

doi:10.1152/jappl.1996.81.6.2658

Cited by 21 publications

(30 citation statements)

References 35 publications

(59 reference statements)

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“…Muscle mass growth (Esser and White 1995) and accumulation of mature (slow) myosin isoform or MHC-1 were decreased by hypokinesia and hypodynamia applied during regeneration of soleus muscle (Bigard et al 1996b;Esser and White 1995). Studies also reported that endurance exercise aects the muscle variables during regeneration (Bigard et al 1996a;Clark et al 1989;Esser and White 1995;White et al 1984). Running on a treadmill enhances the mass and the oxidative metabolism of the regenerating soleus (Clark et al 1989;White et al 1984) and causes a shift from type MHC-2b to type MHC-1 in a regenerating fast-twitch muscle (Bigard et al 1996a).…”

Section: Introductionmentioning

confidence: 99%

Effect of increased physical activity on growth and differentiation of regenerating rat soleus muscle

Ferry¹,

Noirez²,

Salah³

et al. 1997

European Journal of Applied Physiology

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Our purpose was to determine the effect of physical exercise on growth and differentiation during regeneration of a slow-twitch muscle. Degeneration/regeneration of the left soleus muscles of Wistar female rats was induced by injection of a snake venom. Muscular differentiation was studied by monitoring the sequential expression of the various myosin heavy chain isoforms (MHCs). Rats were assigned to one of two groups: cage sedentary (n = 14) or exercised (n = 16). The exercise programme began 1-day post-injection and the rats ran 1 h/day on a motorized treadmill. Then, 9 and 25 days after venom treatment, the soleus MHC phenotype as determined by immunohistology, electrophoresis and immunoblotting, was studied. At 25 days the expression of MHCs by regenerating soleus was not changed by the increased level of physical activity (P > 0.05). Exercised and sedentary regenerating muscles contained similar numbers of type-I fibres (100% of total fibres), levels of MHC-1 (85.4 and 89.5% of total MHCs), MHC-2a and M/HC-2x/d and their fibres expressed MHC-1 (100% of total fibres) and MHC-2 (45-50%) in the same way. Moreover, the masses of regenerating and nonregenerating soleus were significantly increased by physical exercise (P < 0.02). At 9 days no effect of muscular exercise was found. In conclusion, endurance exercise did not alter differentiation of regenerating soleus. Moreover regenerating soleus can respond to increased physical activity by enhancing its mass in the same way as mature muscle.

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Section: Introductionmentioning

confidence: 99%

Effect of increased physical activity on growth and differentiation of regenerating rat soleus muscle

Ferry¹,

Noirez²,

Salah³

et al. 1997

European Journal of Applied Physiology

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“…The muscles of mdx mice are characterized by cycles of muscle fiber necrosis followed by regeneration [Haslett et al, 2005]. The regenerating fibers can be easily identified, because they contain conspicuous internal (central) myonuclei [Bigard et al, 1996]. Williams et al [1993] reported that fibers with either a predominantly centrally located string of myonuclei or a combination of central nuclei and a peripheral pattern of nuclei were noted in soleus muscle fibers of mdx mice.…”

Section: Introductionmentioning

confidence: 99%

Myonucleus-Related Properties in Soleus Muscle Fibers of <i>mdx</i> Mice

Terada

Lan

Kawano

et al. 2009

Cells Tissues Organs

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Distribution and total number of myonuclei in single soleus muscle fibers, sampled from tendon to tendon, were analyzed in mdx and wild-type (WT) mice. Apoptotic myonuclei and the microscopic structure around the myonuclei were also analyzed. Three types of muscle fibers of mdx mice with myonuclear distribution at either central, peripheral, or both central and peripheral regions were observed in the longitudinal analyses. All of the myonuclei were located at the peripheral region in WT mice. The total number of myonuclei counted in the whole length of fibers with peripheral myonuclei only was 17% less in mdx than in WT mice (p < 0.05). But the total myonuclear numbers in mdx mouse fibers with different distribution (peripheral vs. central) of myonuclei were identical, and the peripheral nucleus was noted where the central nucleus was missing. Myonuclei located between the center and peripheral regions were also seen in the cross-sectional analyses of muscle fibers. The cross-sectional area and length of fibers, sarcomere number, myonuclear size, myosin heavy chain expression, satellite cell number and neuromuscular junction were identical between each type of fiber. Apoptosis was not detected in any myonuclei located either in central or peripheral regions of muscle fibers. Thus, it was suggested that apoptosis-related loss of central myonuclei and regeneration-related new accretion at the peripheral region is not the cause of different distribution of myonuclei seen in muscle fibers in mdx mice. However, it was speculated that cross-sectional migration of myonuclei from central to peripheral regions may be induced in response to regeneration, because the total myonuclear numbers in fibers with different distribution of myonuclei were identical, and the peripheral nucleus was noted where the central nucleus was missing. Further, myonuclei located between the center and peripheral regions were also seen. However, the question remains as to how or why nuclei might migrate to the periphery in a regenerating muscle fiber, since there was no microscopic evidence of any structural changes around the myonuclei that may be responsible for the movement of the nucleus.

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“…It is possible that this observation did not reach statistical significance (TG versus CON plantaris: P > 0.05) because of the rapid regeneration of muscle tendon and adhesion of the muscle to surrounding tissues (Goldberg, 1967;Jakubiec-Puka et al 1992). It appears that regenerating fast muscles (the present study ;Bigard et al 1996a) seem to adapt their MyHC expression to increased muscular activity (mechanical overload, locomotion activity) in the same way as mature muscle (Kandanrian et al 1992;Bigard et al 1996a;Harridge, 1996). The effect of tenotomy of the synergistic gastronecmius on regenerating slow muscle seems different from that on the regenerating fast muscle since the relative level of MyHC_1 (the most abundant MHC) was decreased in TG soleus (overloaded regenerating soleus).…”

Section: Discussionmentioning

confidence: 53%

“…Moreover, to our knowledge only one study has analysed the effects of muscular activity on the MyHC expression of a regenerating fast muscle. Bigard et al (1996a) demonstrated that an increased level of locomotion activity (running on a treadmill) modulated the expression of MyHC in the regenerating fast muscle. Thus there is a need for further inquiry into the effects of variation in muscular activity on the MyHC phenotype of fast regenerating muscles.…”

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confidence: 99%

Differential Modification of Myosin Heavy Chain Expression by Tenotomy in Regenerating Fast and Slow Muscles of the Rat

Noirez

Agbulut

Ferry

2000

Experimental Physiology

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We have examined the effect of tenotomy on the expression of myosin heavy chains (MyHC) in regenerating fast and slow skeletal muscles. Degeneration/regeneration of the left soleus and plantaris of Wistar male rats was induced by an injection into the muscle belly of a myotoxin (snake venom: Notechis scutatus scutatus). MyHC isoform content of regenerating plantaris and soleus muscles were studied 21 days after muscle injury using an electrophoretic technique. Tenotomy of the regenerating plantaris (mechanical underload) did not alter its MyHC expression (P > 0.05). In contrast, tenotomy of the regenerating soleus increased its relative levels of MyHC‐2b (P < 0.05) and MyHC‐2x/d (P < 0.01), and decreased its relative level of MyHC‐1 (P < 0.01). Tenotomy of the synergistic gastrocnemius (overload) tended to decrease the relative level of MyHC‐2b in regenerating plantaris (P < 0.07). The effect of tenotomy of the synergistic gastronecmius on the regenerating soleus was different: a decrease in the relative levels of MyHC‐1 (P < 0.05) and an increase in the relative level of MyHC‐neonatal (P < 0.01). In conclusion, and in contrast to a regenerating slow muscle, a change of mechanical loading by tenotomy did not seem to markedly alter the expression of mature MyHC phenotype in a fast regenerating muscle.

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Endurance training affects myosin heavy chain phenotype in regenerating fast-twitch muscle

Cited by 21 publications

References 35 publications

Effect of increased physical activity on growth and differentiation of regenerating rat soleus muscle

Effect of increased physical activity on growth and differentiation of regenerating rat soleus muscle

Myonucleus-Related Properties in Soleus Muscle Fibers of <i>mdx</i> Mice

Differential Modification of Myosin Heavy Chain Expression by Tenotomy in Regenerating Fast and Slow Muscles of the Rat

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