Endovascular Coiling of the Ophthalmic Artery in Pigs to Induce Retinal Ischemia

Morén, Håkan; Gesslein, Bodil; Undrén, Per; Andréasson, Sten; Malmsjö, Malin

doi:10.1167/iovs.11-7628

Cited by 9 publications

(8 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although others have used a porcine model, the marked vascular and ocular anatomical differences favor an NHP model. 16,17 Our studies were exploratory in nature and as such are subject to limitations. Our NHP cohort was limited to six animals.…”

Section: Discussionmentioning

confidence: 99%

Intra-Ophthalmic Artery Chemotherapy Triggers Vascular Toxicity through Endothelial Cell Inflammation and Leukostasis

Steinle

Zhang

Thompson

et al. 2012

Invest. Ophthalmol. Vis. Sci.

View full text Add to dashboard Cite

1,6,9 PURPOSE. Super-selective intra-ophthalmic artery chemotherapy (SSIOAC) is an eye-targeted drug-delivery strategy to treat retinoblastoma, the most prevalent primary ocular malignancy in children. Unfortunately, recent clinical reports associate adverse vascular toxicities with SSIOAC using melphalan, the most commonly used chemotherapeutic. METHODS.To explore reasons for the unexpected vascular toxicities, we examined the effects of melphalan, as well as carboplatin (another chemotherapeutic used with retinoblastoma), in vitro using primary human retinal endothelial cells, and in vivo using a non-human primate model, which allowed us to monitor the retina in real time during SSIOAC.RESULTS. Both melphalan and carboplatin triggered human retinal endothelial cell migration, proliferation, apoptosis, and increased expression of adhesion proteins intracellullar adhesion molecule-1 [ICAM-1] and soluble chemotactic factors (IL-8). Melphalan increased monocytic adhesion to human retinal endothelial cells. Consistent with these in vitro findings, histopathology showed vessel wall endothelial cell changes, leukostasis, and vessel occlusion. CONCLUSIONS.These results reflect a direct interaction of chemotherapeutic drugs with both the vascular endothelium and monocytes. The vascular toxicity may be related to the pH, the pulsatile delivery, or the chemotherapeutic drugs used. Our long-term goal is to determine if changes in the drug of choice and/or delivery procedures will decrease vascular toxicity and lead to better eye-targeted treatment strategies. (Invest Ophthalmol Vis Sci.

show abstract

Section: Discussionmentioning

confidence: 99%

Intra-Ophthalmic Artery Chemotherapy Triggers Vascular Toxicity through Endothelial Cell Inflammation and Leukostasis

Steinle

Zhang

Thompson

et al. 2012

Invest. Ophthalmol. Vis. Sci.

View full text Add to dashboard Cite

show abstract

“…Ciliary artery catheterization is performed with pigs under general anesthesia, positioned on a radiolucent table in the fluoroscopy suite, as described in detail previously (Morén et al, 2011). Briefly, femoral artery access in the inguinal area was obtained using the modified Seldinger technique and a 5 French Stiff Micropuncture Access Set (Cook Medical; G48008 or G35551), which was exchanged for a standard 5 Fr 10 cm length femoral sheath.…”

Section: Methodsmentioning

confidence: 99%

Metabolic Deregulation of the Blood-Outer Retinal Barrier in Retinitis Pigmentosa

et al. 2019

View full text Add to dashboard Cite

SUMMARY Retinitis pigmentosa (RP) initiates with diminished rod photoreceptor function, causing peripheral and nighttime vision loss. However, subsequent loss of cone function and high-resolution daylight and color vision is most debilitating. Visual pigment-rich photoreceptor outer segments (OS) undergo phagocytosis by the retinal pigment epithelium (RPE), and the RPE also acts as a blood-outer retinal barrier transporting nutrients, including glucose, to photoreceptors. We provide evidence that contact between externalized phosphatidylserine (PS) on OS tips and apical RPE receptors activates Akt, linking phagocytosis with glucose transport to photoreceptors for new OS synthesis. As abundant mutant rod OS tips shorten in RP, Akt activation is lost, and onset of glucose metabolism in the RPE and diminished glucose transport combine to cause photoreceptor starvation and accompanying retinal metabolome changes. Subretinal injection of OS tip mimetics displaying PS restores Akt activation, glucose transport, and cone function in end-stage RP after rods are lost.

show abstract

“…[3][4][5][6][7][8] Rats are most widely used in laboratories because their vascular and retinal structures are closer to those of humans, and they are less expensive and are easier to obtain and handle. The most common rat models of retinal ischaemia include pressure elevation, optic nerve ligation, vessel ligation, photodynamic ablation, and endothelin administration.…”

Section: Introductionmentioning

confidence: 99%

Bilateral Common Carotid Artery Occlusion in the Rat as a Model of Retinal Ischaemia

Huang

Fan

et al. 2014

Neuro-Ophthalmology

View full text Add to dashboard Cite

Ocular ischaemic syndrome is a devastating eye disease caused by severe carotid artery stenosis. The purpose of the study was to develop a reliable rat model for this syndrome by means of common carotid artery occlusion and a controllable needle suture method. Adult Wistar rats were subjected to common carotid artery occlusion and sham surgery. The common carotid artery was ligated unilaterally or bilaterally with needles of different diameters, and ocular arterial filling time was examined by fluorescein fundus angiography at different time points. Haematoxylin-eosin staining of vessels and degree of stenosis were considered outcome measures. The ocular blood flow was monitored and measured by laser doppler flowmetry. Needles with a diameter of 0.4 mm were more effective in developing severe stenosis of the common carotid arteries compared with needles of other diameters. Bilateral common carotid artery occlusion was a more effective model than unilateral occlusion. The arterial filling time was significantly increased at 14 and 21 days after ligation (5.75 ± 0.45 and 6.27 ± 0.95 s, respectively) compared with arterial filling time before surgery (5.22 ± 0.64 s). The total blood flow in the sham surgery group was significantly higher than in the bilateral common carotid artery occlusion group. The fundus blood flow was statistically different between the two groups, whereas that of the anterior segment was not. In conclusion, the authors have established a rat model of ocular ischaemic syndrome via a controllable needle suture method, which was reliable up to 2-3 weeks after surgery.

show abstract

Endovascular Coiling of the Ophthalmic Artery in Pigs to Induce Retinal Ischemia

Abstract: The porcine ophthalmic artery can be occluded using an endovascular coiling technique. This provides an experimental animal model of retinal ischemia in which occlusion at different sites of the vasculature produces different degrees of severity of the ischemic damage.

Cited by 9 publications

References 22 publications

Intra-Ophthalmic Artery Chemotherapy Triggers Vascular Toxicity through Endothelial Cell Inflammation and Leukostasis

Intra-Ophthalmic Artery Chemotherapy Triggers Vascular Toxicity through Endothelial Cell Inflammation and Leukostasis

Metabolic Deregulation of the Blood-Outer Retinal Barrier in Retinitis Pigmentosa

Bilateral Common Carotid Artery Occlusion in the Rat as a Model of Retinal Ischaemia

Contact Info

Product

Resources

About