Endotoxin-inactivating Potency of Hydrogen Peroxide: Effect on Cell Growth

DeRenzis, Frank A.

doi:10.1177/00220345810600051401

Cited by 9 publications

(3 citation statements)

References 19 publications

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“…Because the lifetime of •OH is very short ( 9), main detoxification of endotoxin proceeds on the surface of TiO2 thin film. However, it is known that lipid A of the endotoxin is also oxidized by hydrogen peroxide (H2O2) (20). Therefore, the possibility that some inactive rections proceed in the solution phase cannot be excluded in the present conditions.…”

Section: Reductive Reactionsmentioning

confidence: 81%

Bactericidal and Detoxification Effects of TiO₂ Thin Film Photocatalysts

Sunada

Kikuchi

Hashimoto

et al. 1998

Environ. Sci. Technol.

792

424

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To examine the special features of the antibacterial effect for a thin transparent titanium dioxide (TiO2) film, the photocatalytic degradation of endotoxin, which is a pyrogenic constituent of Escherichia coli (E. coli), as well as its bactericidal activity, was investigated. The TiO2 films were prepared from titanium isopropoxide solution, annealing at 500 °C. The bactericidal activity for E. coli cells was estimated by survival ratio calculated from the number of viable cells which form colonies on the nutrient agar plates. The endotoxin concentration was determined by the Limulus tests. When E. coli cells were killed by the TiO2 photocatalyst under UV irradiation, the endotoxin from the cells was also degraded efficiently. This result shows that the TiO2 photocatalyst has both bactericidal activity and decomposing activity for the endotoxin (i.e., detoxifying activity). The bactericidal effect of the TiO2 thin film results from both inactivating the viability of the bacteria and the destruction of the E. coli cells. This feature renders TiO2 photocatalysts to be applicable to environmental protections, especially in medical facilities where the endotoxin is needed to control.

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Section: Reductive Reactionsmentioning

confidence: 81%

Bactericidal and Detoxification Effects of TiO₂ Thin Film Photocatalysts

Sunada

Kikuchi

Hashimoto

et al. 1998

Environ. Sci. Technol.

792

424

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“…Nakib, et al (1982) Commercially obtained endotoxin may not significantly affect the attachment of fibroblasts but may only affect their growth (Bergman and Nilsson, 1963;DeRenzis, 1981;and Olson, Adams and Layman, 1985) or both growth and viability (Aleo, et al 1974;Singer and Dutton, 1979).…”

mentioning

confidence: 99%

The Effects of the Nd:YAG Laser on in Vitro Fibroblast Attachment to Endotoxin‐Treated Root Surfaces

Trylovich¹,

Cobb

Pippin

et al. 1992

Journal of Periodontology

103

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yttrium, aluminum, garnet (Nd:YAG) laser using a 320 micron contact optic fiber handpiece with an energy setting of 80 mJ at 10 pulses per second for one minute.The fiber was held perpendicular to the root surface. An attempt was made to cover the entire root surface equally during the one minute of exposure. This was confirmed in a subsequent pilot study using lased, non-diseased root segments.A pilot study using photoacoustic spectroscopy on a iii laser charred root surface revealed the presence of a charged ion of ammonium and an altered phosphate to carbonate ratio as compared to a non-lased root segment.The presence of this charged ion of ammonium may have

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“…Associando ao conhecimento expresso por Nilsson e col. 11 , que enfatizam a importância da análise de endotoxinas ligadas à superfície polimérica, que ainda assim induzem a reações de ativação do complemento, justifica atenção particular a este segmento, buscando a garantia na liberação destes correlatos livres da presença de endotoxinas. O peróxido de hidrogênio foi há muitos anos considerado efetivo na destruição de endotoxinas, dependendo de concentração, tempo de contato e temperatura 2,10,16 , embora sem elucidação completa do mecanismo da reação 3,6,7 . Na aplicação proposta inexistem riscos de incompatibilidade, sendo resíduo único a umidade, eliminada quando da secagem final dos componentes.…”

Section: Discussionunclassified

Peróxido de hidrogênio como agente despirogenizante de componentes para produtos médico-hospitalares

Pinto

1995

Rev. Saúde Pública

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The current search for alternative solvents to fluorinated products, in face of the depletion of ozone in the stratosphere calls for the development of alternative processes applicable to the manufacture of medical devices. In view of the challenge and as water is serving particularly as the choice for other industrial segments, the fast macrobial proliferation in it is of concern, as it is a potential source of endotoxins. Such a risk is inconsistent with the production of items designed for example, for use in surgical procedures in the cardio-vascular field. Thus research was undertaken for the purpose of investigating the possibility of using water as a cleansing agent for components for such products, providing that hydrogen peroxide is added. The work was carried out by inoculating water and peroxide at levels of 0.1, 0.25, 0.5 e 1.0 UE/ml. The confirmation was obtained as to the active effectiveness at a 5% concentration of hydrogen peroxide by means of analytical determination using the "in vitro" method. The effectiveness of the use of peroxide was investigated on polycarbonate injected parts designed for the manufacture of oxigenators and blood reservoirs and contained with endotoxins. The findings permit one to draw a favorable conclusion regarding the adequacy of the proposed process both biologically as well as regards the removal of impurities.

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Endotoxin-inactivating Potency of Hydrogen Peroxide: Effect on Cell Growth

Cited by 9 publications

References 19 publications

Bactericidal and Detoxification Effects of TiO₂ Thin Film Photocatalysts

Bactericidal and Detoxification Effects of TiO₂ Thin Film Photocatalysts

The Effects of the Nd:YAG Laser on in Vitro Fibroblast Attachment to Endotoxin‐Treated Root Surfaces

Peróxido de hidrogênio como agente despirogenizante de componentes para produtos médico-hospitalares

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Endotoxin-inactivating Potency of Hydrogen Peroxide: Effect on Cell Growth

Cited by 9 publications

References 19 publications

Bactericidal and Detoxification Effects of TiO2 Thin Film Photocatalysts

Bactericidal and Detoxification Effects of TiO2 Thin Film Photocatalysts

The Effects of the Nd:YAG Laser on in Vitro Fibroblast Attachment to Endotoxin‐Treated Root Surfaces

Peróxido de hidrogênio como agente despirogenizante de componentes para produtos médico-hospitalares

Contact Info

Product

Resources

About

Bactericidal and Detoxification Effects of TiO₂ Thin Film Photocatalysts

Bactericidal and Detoxification Effects of TiO₂ Thin Film Photocatalysts