Endotoxin detection in full blood plasma in a theranostic approach to combat sepsis

Sheikh, Sonia; Blaszykowski, Christophe; Romaschin, Alexander D.; Thompson, Michael

doi:10.1039/c6ra02745h

Cited by 5 publications

(1 citation statement)

References 40 publications

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“…As the EMPAS, unlike the QCM, does not require electrode connections, the homogenous surface of the quartz sensor allows for versatile surface chemistry routes to engineer the surface for a given interaction. EMPAS devices have been used for detection of HIV-2 antibodies in human serum [ 17 ], endotoxin of pathogenic E. coli in full human blood plasma [ 18 ], cocaine [ 19 ], breast and prostate cancer metastasis biomarker (PTHrP) [ 20 ]. They have also been used in the study of antifouling coating against undiluted goat serum [ 21 ], full human blood plasma [ 22 ], human serum and bovine milk [ 23 ], as well as in the determination of plasmin enzyme of pM concentration levels [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

Electromagnetic Piezoelectric Acoustic Sensor Detection of Extracellular Vesicles through Interaction with Detached Vesicle Proteins

et al. 2020

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An electromagnetic piezoelectric acoustic sensor (EMPAS) was used to study the non-specific adsorption of human red blood cell-derived extracellular vesicle preparations. Vesicle storage history (temperature and duration) highly affected the obtained results: The signal change, namely the frequency decrease of the crystal measured at 20 °C, was negligibly small (<1 s−2) when the vesicle solutions had previously been stored at 4 °C, and was in the order of 10 s−2 when the vesicle solutions had been stored at −30 °C. Moreover, the rate of frequency decrease increased exponentially with the storage time at −30 °C. Upon a 4 °C storage period following the −30 °C storage period of the same sample, the measured frequency decrease dropped, suggesting a partial relaxation of the system. The results are explained by the disintegration of the vesicles triggered by the freeze–thaw cycle, likely due to the detachment of proteins from the vesicle surface as was proved by size-exclusion chromatography. Surface modification of the sensor crystal provided the possibility of signal enhancement, as the maximum rate of the frequency change for the same vesicle concentrations was higher on hydrophobic, octadecyl trichlorosilane–modified quartz than on hydrophilic, bare quartz. The EMPAS signal has been associated with the amount of detached proteins, which in turn is proportional to the originating vesicle concentration.

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Section: Introductionmentioning

confidence: 99%

Electromagnetic Piezoelectric Acoustic Sensor Detection of Extracellular Vesicles through Interaction with Detached Vesicle Proteins

et al. 2020

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Current technologies to endotoxin detection and removal for biopharmaceutical purification

Schneier

Razdan

Miller

et al. 2020

Biotech & Bioengineering

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Endotoxins are the major contributors to the pyrogenic response caused by contaminated pharmaceutical products, formulation ingredients, and medical devices. Recombinant biopharmaceutical products are manufactured using living organisms, including Gram‐negative bacteria. Upon the death of a Gram‐negative bacterium, endotoxins (also known as lipopolysaccharides) in the outer cell membrane are released into the lysate where they can interact with and form bonds with biomolecules, including target therapeutic compounds. Endotoxin contamination of biologic products may also occur through water, raw materials such as excipients, media, additives, sera, equipment, containers closure systems, and expression systems used in manufacturing. The manufacturing process is, therefore, in critical need of methods to reduce and remove endotoxins by monitoring raw materials and in‐process intermediates at critical steps, in addition to final drug product release testing. This review paper highlights a discussion on three major topics about endotoxin detection techniques, upstream processes for the production of therapeutic molecules, and downstream processes to eliminate endotoxins during product purification. Finally, we have evaluated the effectiveness of endotoxin removal processes from a perspective of high purity and low cost.

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Electrochemical aptasensor for ultrasensitive detection of lipopolysaccharide using silver nanoparticles decorated titanium dioxide nanotube/functionalized reduced graphene oxide as a new redox nanoprobe

Tian

Wang

et al. 2021

Microchim Acta

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Endotoxin detection in full blood plasma in a theranostic approach to combat sepsis

Abstract: A qualitative piezoelectric biosensor assay alternative for the detection of bacterial endotoxin is described to perform measurements in full human blood plasma in a real-time and label-free advanced fashion.

Cited by 5 publications

References 40 publications

Electromagnetic Piezoelectric Acoustic Sensor Detection of Extracellular Vesicles through Interaction with Detached Vesicle Proteins

Electromagnetic Piezoelectric Acoustic Sensor Detection of Extracellular Vesicles through Interaction with Detached Vesicle Proteins

Current technologies to endotoxin detection and removal for biopharmaceutical purification

Electrochemical aptasensor for ultrasensitive detection of lipopolysaccharide using silver nanoparticles decorated titanium dioxide nanotube/functionalized reduced graphene oxide as a new redox nanoprobe

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