Endotoxin, cat, and house dust mite allergens in electrostatic cloths and bedroom dust

Effectiveness of portable HEPA air cleaners on reducing indoor endotoxin, PM 10, and coarse particulate matter in an agricultural cohort of children with asthma: A randomized intervention trial

“…The geometric mean endotoxin loadings we measured in HAPI homes were similar to those of other studies using EDCs with two‐week deployments, including studies of farm homes in Iowa, USA, 75,77 homes near livestock farms in dense agricultural regions of the Netherlands, 44 and non‐smoking urban apartments and homes in Denmark, 43,102 and the Netherlands, 103 and slightly higher than those in 60 homes of children in Wellington, NZ using four‐week EDC deployments 104 . One EDC study found higher endotoxin loadings in farm vs. non‐farm homes 105 .…”

Metagenomic Characterization of Indoor Dust Bacterial and Fungal Microbiota in Homes of Asthma and Non-asthma Patients Using Next Generation Sequencing

“…Because our objective for this work was to use NGS, we decided to sample dust by vacuuming the floor rather than aerosols in order to gain in sensitivity. Another valuable technical option described in the literature relies on the use of electrostatic dust cloths for further molecular, immunological or cultural analysis (Cox et al, 2017;Kristono et al, 2019).…”

Effect of air filtration on house dust mite, cat and dog allergens and particulate matter in homes

“…Despite the use of HEPA air purifiers in previous studies to assess their effect on HDM, 39,40,50 dog 48,61-63 and cat 34,39,48,[61][62][63][64] allergen exposure, these were either restricted to one 34,39,61,64 or two allergens, 37,38,62,63 based on one home only 50 or did not measure airborne allergen levels. 37,38,[62][63][64] For instance, Jia-Ying et al 40 showed a decrease in HDM allergens similar to the percentage reported in our study, but analyzing bedding and static dust samples (68.3% and 71.0%) and not airborne samples.…”

“…Despite the use of HEPA air purifiers in previous studies to assess their effect on HDM, 39 , 40 , 50 dog 48 , 61 , 62 , 63 and cat 34 , 39 , 48 , 61 , 62 , 63 , 64 allergen exposure, these were either restricted to one 34 , 39 , 61 , 64 or two allergens, 37 , 38 , 62 , 63 based on one home only 50 or did not measure airborne allergen levels. 37 , 38 , 62 , 63 , 64 For instance, Jia‐Ying et al.…”

“… 47 Here HDM allergens D. pteronyssinus Extract FD (Fetal Distress) excrements (Der f 1 < 0,05/Der p 2 = 0,78/Der p 1 = 22,3 mg/g), D. pteronyssinus Whole Cultures (Der f 1 = 0/Der p 2 = 0,64/Der p 1 = 6,74 mg/g), and D. farinae Whole Cultures (Der f 1 = 1,86/Der f 2 = 0,30/Der p 1 = 0 mg/g) from Cyteq Biologics, Groningen, Netherlands or EDC (Electrostatic dust collector)‐samples from homes were used. 46 , 48 All samples were extracted with 0.1 M ammonium bicarbonate pH = 8.0 containing 0.1% BSA, lyophilised, stored at −80°C and re‐suspended before analysis in 1/10 of the original volume in 0.1 M PBS pH = 7.2 + 0.05% Tween and 1% BSA. The limit of quantification (LOQ) in the calibration curve was 0.096 pg/m 3 for Der f 1, 6.14 pg/m 3 for Der p 1, 0.12 pg/m 3 for Fel d 1 and 1.76 pg/m 3 for Can f 1.…”