Endotoxemia in liver diseases: Detection by a quantitative assay using chromogenic substrate with perchloric acid pretreatment.

Endotoxemia Analysis by the Limulus Amoebocyte Lysate Assay in Different Mammal Species Used in Metabolic Studies

“…In fact the samples of a plate will be validated only if the negative control and the standard curve respect the pronounced norms in the materials and methods. Other studies have compared the preparation of the standard curve in LAL water and in plasma [15,26]. In our case and using Cape Cod reagent we performed the standard curve in LAL water.…”

“…For pigs, the recovery of spiked endotoxin was 118% for dilutions of 1/100 and 1/200. We have also calculated for all samples for which we have measured endotoxemia (human, rodent, pig), the proportion of samples with (i) a within-triplicate variation coefficient >25% and [15] a spike recovery <50% or >200%. As reported Table 1, it is interesting to note that around 10% of the spike recoveries of all species samples are <50% except for mice where the % of samples which have an inhibitory spike recovery (<50%) is 30% (Table 1).…”

“…The presence of an inhibition can be detected when endotoxins added to serum or plasma (positive product control, so-called "spike") fails to react with the LAL reagent. Several methods have been used to remove inhibition of the LAL test such as dilution, heating [4,14], solvent extraction [11] and acid treatment [14,15]. The combination of dilution and heating processes has been shown to be efficacious, yet we have also added a sonication step as the extraction of LPS is also influenced significantly by physical characteristics that determine its aggregation [4,16].…”

A case of liver cirrhosis complicated with endotoxemia and disseminated intravascular coagulation : A case report.

“…We have already reported endotoxemia in liver diseases with a special reference to the close relationship between endotoxemia and the development of DIC (Yajima et al 1985). In fulminant hepatic failure, the close correlation between the occurrence of endotoxemia and the development of DIC is known (Wilkinson et al 1974).…”

Quantitative assay of lentinan in human blood with the limulus colorimetric test.

“…On the other hand, Endospecy® is an endotoxin-specific LCT kit in which f3-glucan sensitive factor (0-factor) is selectively eliminated from its lysate (Obayashi et al 1985). Details of LCT are described elsewhere (Yajima et al 1985) Administration of lentinan Lentinan (Yamanouchi Pharmaceuticals Co., Ltd., Tokyo) was dissolved in 500 ml of 5% glucose solution and administered intravenously for 2 hr.…”