Endothelium-Dependent Hyperpolarization: Out of the Dish and into the Brain

Bryan, Robert M.; Marrelli, Sean P.

doi:10.1038/jcbfm.2010.221

Cited by 3 publications

(3 citation statements)

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Section: Osa Attenuates Oscillations Of the Circadian Clock In Cerebral Arteries But Not The Heartmentioning

confidence: 99%

“…ATP has been shown to dilate cerebral arteries through a NO synthase cyclooxygenase independent mechanism known as endothelium dependent hyperpolarizations (EDH). [34][35][36] To examine the EDH component of dilation contributing to the time-ofday-dependent rhythm observed in sham and OSA rats (Figure 5(e)), dilations to ATP in the presence of L-NAME (inhibits NO synthase; 100 mM) and indomethacin (inhibits cyclooxygenase; 10 mM) were examined over the 24-h period in PCAs. Supplemental Figure 1(b) illustrates the constriction of sham and OSA PCAs to L-NAME and indomethacin pretreatment over the course of 24 h. Although two-way ANOVA did not indicate any significant differences, sham PCAs exhibited greater constrictions to L-NAME and indomethacin at ZT0 versus ZT12.…”

Section: Osa Attenuates Oscillations Of the Circadian Clock In Cerebr...mentioning

confidence: 99%

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The rat cerebral vasculature exhibits time-of-day-dependent oscillations in circadian clock genes and vascular function that are attenuated following obstructive sleep apnea

Durgan

Crossland

Bryan

2016

J Cereb Blood Flow Metab

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Circadian clock components oscillate in cells of the cardiovascular system. Disruption of these oscillations has been observed in cardiovascular diseases. We hypothesized that obstructive sleep apnea, which is associated with cerebrovascular diseases, disrupts the cerebrovascular circadian clock and rhythms in vascular function. Apneas were produced in rats during sleep. Following two weeks of sham or obstructive sleep apnea, cerebral arteries were isolated over 24 h for mRNA and functional analysis. mRNA expression of clock genes exhibited 24-h rhythms in cerebral arteries of sham rats (p < 0.05). Interestingly, peak expression of clock genes was significantly lower following obstructive sleep apnea (p < 0.05). Obstructive sleep apnea did not alter clock genes in the heart, or rhythms in locomotor activity. Isolated posterior cerebral arteries from sham rats exhibited a diurnal rhythm in sensitivity to luminally applied ATP, being most responsive at the beginning of the active phase (p < 0.05). This rhythm was absent in arteries from obstructive sleep apnea rats (p < 0.05). Rhythms in ATP sensitivity in sham vessels were absent, and not different from obstructive sleep apnea, following treatment with L-NAME and indomethacin. We conclude that cerebral arteries possess a functional circadian clock and exhibit a diurnal rhythm in vasoreactivity to ATP. Obstructive sleep apnea attenuates these rhythms in cerebral arteries, potentially contributing to obstructive sleep apnea-associated cerebrovascular disease.

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Section: Osa Attenuates Oscillations Of the Circadian Clock In Cerebral Arteries But Not The Heartmentioning

confidence: 99%

Section: Osa Attenuates Oscillations Of the Circadian Clock In Cerebr...mentioning

confidence: 99%

The rat cerebral vasculature exhibits time-of-day-dependent oscillations in circadian clock genes and vascular function that are attenuated following obstructive sleep apnea

Durgan

Crossland

Bryan

2016

J Cereb Blood Flow Metab

Self Cite

View full text Add to dashboard Cite

show abstract

“…Dilations in isolated pressurized middle cerebral arteries, elicited by stimulating endothelial P2Y 2 receptors by adenosine triphosphate, were attenuated by %40% compared to a control group that was surgically prepared but did not undergo apnea. Although the dilation in response to adenosine triphosphate was the result of multiple endothelial-dependent mechanisms of dilation, [152][153][154][155] the attenuated response was due to suppression of the NO component. 151 Thus, there is continuity with the findings from different animal models for OSA, intermittent hypoxia, and apnea.…”

Section: Cellular and Molecular Mechanismsmentioning

confidence: 99%