Endothelium-Dep Endent Calcium Fluxes in Rabbit Aorta

Collins, P.; Griffith, T.; Mj, Lewis; Henderson, A. H.

doi:10.1042/cs067028p

Cited by 2 publications

(3 citation statements)

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“…Calcium influx was slightly but significantly higher with than without endothelium both in the absence and presence of noradrenaline. As previously reported (Collins, Griffith, Henderson & Lewis, 1985), this endothelium-dependent increment was abolished by flurbiprofen but not by the EDRF inhibitors dithiothreitol, potassium borohydride or phenidone (Griffith et al 1984a) (data not shown). Acetylcholine reduced noradrenaline-stimulated calcium influx in the presence of endothelium but had no effect in the absence of endothelium (Fig.…”

Section: Effect Of Endothelium On Noradrenaline-stimulated Calcium Insupporting

confidence: 87%

“…It is now apparent that endothelium produces a potent vasodilator agent which is likely to prove of major importance in mediating and co-ordinating many ofthe fundamental phenomena ofvascular control (Furchgott, 1983; Griffith, Edwards, Collins, Lewis & Henderson, 1985a). Although the responsible agent, endothelium-derived relaxing factor (EDRF), has yet to be chemically identified, it is known to be an unstable, novel biological compound which is continually produced in the resting state under experimental conditions (Griffith, Edwards, Lewis, Newby & Henderson, 1984a;Griffith, Henderson, Hughes-Edwards & Lewis, 1984b) and whose production can be stimulated by a number of physiologically relevant substances (for review see Furchgott, 1983;and Griffith et al 1985 a).…”

Section: Introductionmentioning

confidence: 99%

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Endothelium‐derived relaxing factor alters calcium fluxes in rabbit aorta: a cyclic guanosine monophosphate‐mediated effect.

Collins¹,

Griffith²,

Henderson³

et al. 1986

The Journal of Physiology

125

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SUMMARY1. Measurement of tension and 45Ca influx and efflux were used to study the effects of endothelium-derived relaxing factor (EDRF), sodium nitroprusside and 8-bromocyclic guanosine monophosphate (GMP) on contractile responses and calcium movements in aortic ring preparations of the rabbit.2. EDRF activity, induced by stimulating endothelium-containing rings with acetylcholine, was associated with relaxation of noradrenaline-constricted rings and with a marked reduction of noradrenaline-stimulated increase in calcium influx.Sodium nitroprusside and 8-bromo-cyclic GMP had a similar effect in deendothelialized preparations.3. EDRF also inhibited noradrenaline-stimulated calcium efflux. Sodium nitroprusside and 8-bromo-cyclic GMP had a similar effect in de-endothelialized preparations, both in the presence and absence of extracellular calcium.4. The vascular smooth muscle relaxant effect of EDRF and of nitrovasodilators may be effected by a cyclic GMP-mediated reduction of cytosolic calcium, through both inhibition of calcium influx and reduction of intracellular calcium release.

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Section: Effect Of Endothelium On Noradrenaline-stimulated Calcium Insupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Endothelium‐derived relaxing factor alters calcium fluxes in rabbit aorta: a cyclic guanosine monophosphate‐mediated effect.

Collins¹,

Griffith²,

Henderson³

et al. 1986

The Journal of Physiology

125

View full text Add to dashboard Cite

show abstract

“…This suggests that cyclic GMP might also regulate the opening ofthe Ca2+ channel in smooth muscle thereby modulating the Ca2+ gating mechanism activated by noradrenaline. This interpretation is in agreement with observations on the role ofendothelium in Ca2+ fluxes where it has been shown that the presence of endothelium reduces noradrenaline-dependent Ca2+ influx in rat and rabbit aortae Collins et al, 1985;Malta et al, 1986b).…”

Section: Discussionsupporting

confidence: 81%

Role of cyclic GMP in the modulation by endothelium of the adrenolytic action of prazosin in the rat isolated aorta

Alosachie

Godfraind

1986

British J Pharmacology

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1 The effect ofendothelium on the adrenolytic action ofprazosin was studied in the rat isolated aorta. 2 Prazosin showed a non-competitive type of antagonism in preparations with intact endothelium while in preparations where endothelium had been removed, prazosin at concentrations between 0.3 nM-10 nM acted as a competitive antagonist. 3 Methylene blue, used to decrease tissue levels of guanosine 3': 5'-cyclic monophosphate (cyclic GMP), converted prazosin from a non-competitive antagonist into an apparently competitive antagonist in the presence of endothelium. 4 Increasing tissue levels ofcyclic GMP by incubation with 8-bromo-cyclic GMP converted prazosin from an apparently competitive antagonist into a non-competitive antagonist in the absence of endothelium. 5Analysis of concentration-response curves for noradrenaline in the presence and absence of endothelium showed that the affinity for noradrenaline was the same but the efficacy, measured by estimating the receptor reserve, was not; it was lower in the presence than in the absence of endothelium. 6 It was concluded that the change in the mode of antagonism of prazosin after endothelium removal could be related to an alteration in the efficacy of the agonist, brought about by a change in the tissue levels of cyclic GMP.

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Endothelium-Dep Endent Calcium Fluxes in Rabbit Aorta

Cited by 2 publications

References 0 publications

Endothelium‐derived relaxing factor alters calcium fluxes in rabbit aorta: a cyclic guanosine monophosphate‐mediated effect.

Endothelium‐derived relaxing factor alters calcium fluxes in rabbit aorta: a cyclic guanosine monophosphate‐mediated effect.

Role of cyclic GMP in the modulation by endothelium of the adrenolytic action of prazosin in the rat isolated aorta

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