Endothelins Inhibit Luteinization of Cultured Porcine Granulosa Cells*

Iwai, Mieko; Hasegawa, Masaaki; Taii, Shunzo; Sagawa, Norimasa; Nakao, Kazuwa; Imura, Hiroo; Nakanishi, Shigetada; Mori, Takahide

doi:10.1210/endo-129-4-1909

Cited by 60 publications

(52 citation statements)

References 21 publications

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“…It has been reported that endothelial cells constitute the major proportion (53.5%) of CL cells [23,24], and Girsh et al [25] reported that progesterone production by luteinized bovine granulosa and theca cells was modulated when co-cultured with endothelial cells. Furthermore, endothelial cells secrete prostaglandin I 2 [26] and endothelin-1 [27], which have been reported to affect steroidogenesis [28][29][30]. These data suggest that the luteotropic effect of OT might be modulated by unknown factors produced by nonsteroidogenic cells in this tissue culture system.…”

Section: Discussionmentioning

confidence: 54%

Progesterone Release of Bovine Corpus Luteum in Response to Oxytocin in Different Culture Systems.

Sakumoto

Ando

Okuda

1996

Journal of Reproduction and Development

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Abstract. The aim of the present study was to investigate the effect of oxytocin (OT) on progesterone secretion by bovine corpus luteum (CL) in different culture systems. When luteal cells were cultured for 1, 2, 4 and 15 h at a density of 6 × 10 4 cells/ml, OT (10 -7 M) did not affect progesterone secretion. On the other hand, bovine LH strongly stimulated progesterone secretion by the cells 2-15 h after incubation (P<0.01). In another experiment, a novel luteal slice culture system was used, in which luteal cells maintained cell-to-cell contact. Three slices (15-20 mg/a slice) from a CL were pre-incubated for 1 h, and then each slice was incubated for 1 h in a culture tube with a medium (3 ml) supplemented with and without bovine LH (100 ng/ml) or OT (10 -7 M). Slices were replaced every 1 h in a fresh medium during the 5 h incubation experiment. A 1-h stimulation with bovine LH and OT induced significant but different effects on the release of progesterone. OT acutely stimulated progesterone release at doses 10 -7 M (155%; P<0.05), and bovine LH significantly increased progesterone release during and after stimulation (223%; P<0.01). These data support the hypothesis that OT plays a role in modulating the mechanisms of progesterone secretion as an intraluteal regulator. Differences observed between the two systems might be due to the presence or absence of cell-to-cell contact. Key words: Oxytocin, Cell-to-cell contact, Corpus luteum, Bovine.(J. Reprod. Dev. 42 : 199-204, 1996) Accepted for publication: June 3, 1996 Correspondence: K. Okuda t is well known that oxytocin (OT) is synthesized and secreted by the corpus luteum (CL) a luteolytic effect [12], or no effect [13,14]. These different results obtained from in vitro studies seem to depend on the experimental model, e.g., shortor long-term culture of luteal cells, cell density and duration of stimulation with reagents. Recently, it has been reported that the communication between large and small luteal cells is very important for the function of the CL [15]. In addition, Miyamoto et al. [16] reported that OT stimulated progesterone production from bovine CL using an in vitro microdialysis system (MDS). In contrast to classical cell culture techniques, MDS maintains cell-to-cell contact as is the case in vivo. Thus, it is strongly suggested that cell-to-cell contact may be a requisite for the CL response to OT.The present study was conducted to investigate I in many species [1][2][3][4][5][6][7]. Moreover, the presence of OT receptors in the CL has been demonstrated in the cow [8], pig [9] and sheep [10]. Although these data suggest that luteal OT plays an important physiological role as an autocrine or paracrine regulator or both, a satisfactory explanation of the physiological role of OT in the CL has not been provided. In vitro models, which are mainly based on the incubation of dispersed luteal cells, have shown a variety of CL responses to OT in terms of progesterone production: a luteotropic effect [11],

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Section: Discussionmentioning

confidence: 54%

Progesterone Release of Bovine Corpus Luteum in Response to Oxytocin in Different Culture Systems.

Sakumoto

Ando

Okuda

1996

Journal of Reproduction and Development

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“…The effects of ET-1 on intracellular cAMP levels appear to be cell type-dependent, possibly related to coupling of ET A and ET B receptors to adenylyl cyclases via different G-proteins. 52,53 Thus, ET-1 inhibits activator-induced cAMP increase in bovine EC, 52 astrocytes, 54 rat glioma cells, 55 Swiss 3T3 cells, 56 pig granulosa cells, 57 and in rat nephron segments. 58 ET-1-induced elevation of cAMP was observed in platelets.…”

Section: Discussionmentioning

confidence: 92%

Endothelin-1 Enhances Plasminogen Activator Inhibitor-1 Production by Human Brain Endothelial Cells via Protein Kinase C-Dependent Pathway

Zidovetzki

Wang

Kim

et al. 1999

ATVB

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The effects of endothelin-1 (ET-1) on the production of plasminogen activator inhibitor 1 (PAI-1) and tissue plasminogen activator (t-PA) by human brain-derived endothelial cells in culture were studied. At 100 nmol/L, ET-1 increased PAI-1 production by 88+/-6% within 72 hours, and increased PAI-1 mRNA expression within 1 hour of stimulation; there was no significant effect on t-PA production. PAI-1 activity was also examined and found to increase with ET-1 treatment. Suboptimal concentrations of ET-1 and tumor necrosis factor-alpha (TNF-alpha) acted synergistically to increase PAI-1 production. ET-1 activated protein kinase C and cAMP-dependent protein kinase pathways within 3 to 5 minutes of treatment, with the peak at 10 minutes. Activation of protein kinase C by phorbol-12-myristate-13-acetate (PMA) resulted in increased PAI-1 production, whereas activation of the cAMP-dependent protein kinase by forskolin or dibutyryl cAMP (dBu-cAMP) significantly decreased PAI-1 production. However, simultaneous activation of protein kinase C by PMA and cAMP-dependent protein kinase by dBu-cAMP only slightly attenuated PMA-induced PAI-1 increase. Inhibition of protein kinase C by GF-109213X abolished the effects of ET-1. These results demonstrate that ET-1 and TNF-alpha function synergistically to induce procoagulant activity of brain endothelial cells in a process that involves a protein kinase C-dependent pathway.

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“…ETs also influence steroidogenesis through the activation of ETA (Iwai et al 1991, Tedeschi et al 1994, Kamada et al 1995, Girsh et al 1996. ET1 was shown to inhibit LH-, FSH-and hCG-stimulated P4 secretion by porcine, human and rat granulosa cells, as well as by cultured bovine luteal cells (Iwai et al 1991, Flores et al 1992, Tedeschi et al 1994, Kamada et al 1995, Girsh et al 1996. Furthermore, as shown in cattle (Ohtani et al 1998), ETs seem to be involved in PGF2a-induced luteolysis.…”

Section: Introductionmentioning

confidence: 93%

“…The functional importance of ET2 and ETB for ovulation, CL formation and luteal P4 production was shown in mice (Palanisamy et al 2006, Cacioppo et al 2014. ETs also influence steroidogenesis through the activation of ETA (Iwai et al 1991, Tedeschi et al 1994, Kamada et al 1995, Girsh et al 1996. ET1 was shown to inhibit LH-, FSH-and hCG-stimulated P4 secretion by porcine, human and rat granulosa cells, as well as by cultured bovine luteal cells (Iwai et al 1991, Flores et al 1992, Tedeschi et al 1994, Kamada et al 1995, Girsh et al 1996.…”

Section: Introductionmentioning

confidence: 98%

Expression and functional implications of luteal endothelins in pregnant and non-pregnant dogs

Gram¹,

Latter²,

Boos³

et al. 2015

Reproduction

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Luteal development is regulated by many locally produced mediators, e.g., prostaglandins and angiogenic factors. However, the role and function of vasoactive factors in the canine corpus luteum (CL) remain largely unknown. Consequently, expression of the endothelin (ET) receptors-A and -B (ETA and ETB, revealing vasoconstriction and vasodilator properties respectively), the ET-converting enzyme (ECE1) and ET1, -2 and -3 were investigated in CL from non-pregnant dogs (days 5, 15, 25, 35, 45 and 65 post-ovulation), and at selected stages of pregnancy (pre-implantation, post-implantation, mid-gestation), and during normal and antigestagen-induced prepartum luteolysis/ abortion. The interrelationship between PGE2 and the ET system was investigated in PGE2-treated canine primary lutein cells from early CL. ET1 did not change significantly over time; ET2, ECE1 and ETB were elevated in early CL and were downregulated towards the mid/late-luteal phase. The prepartum increase of ET2 was significant. ET3 increased gradually, and was highest in late CL and/or at prepartum luteolysis. ETA remained constant until the late CL phase and increased only during prepartum luteolysis. ET1 was localized to the luteal cells, and ET2, ET3 and ETA to vascular endothelium. ECE1 and ETB were detected at both locations. Except for upregulated ET1 and lack of effect on ET2, antigestagen applied to mid-pregnant dogs evoked similar changes to those observed during normal luteolysis. PGE2 upregulated ETB in treated cells; ETA and ET1 remained unaffected, and ET2 decreased. A modulatory role of the ETs in canine CL, possibly in association with other factors (e.g., PGE2 and progesterone receptor), is strongly indicated.Reproduction (2015) 150 405-415

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Endothelins Inhibit Luteinization of Cultured Porcine Granulosa Cells*

Cited by 60 publications

References 21 publications

Progesterone Release of Bovine Corpus Luteum in Response to Oxytocin in Different Culture Systems.

Progesterone Release of Bovine Corpus Luteum in Response to Oxytocin in Different Culture Systems.

Endothelin-1 Enhances Plasminogen Activator Inhibitor-1 Production by Human Brain Endothelial Cells via Protein Kinase C-Dependent Pathway

Expression and functional implications of luteal endothelins in pregnant and non-pregnant dogs

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