Endothelin production by amnion and its regulation by cytokines

Mitchell, Murray D.; Lundin-Schiller, Sarah; Edwin, Samuel S.

doi:10.1016/0002-9378(91)90238-m

Cited by 31 publications

(19 citation statements)

References 16 publications

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“…2, cultured human amnion cells produced and secreted large amounts of ET-1-LI in a time-dependent manner, and the major constituent of ET-1-LI in the culture media was ET-1 [8]. However, cells derived from chorion laeve or decidua vera tissue did not secrete ET-1-LI [8,9]. The expression of prepro-ET-1 mRNA was detected in amniotic tissue and cultured amnion cells but not in decidua tissue [6,7].…”

Section: Secretion Of Et-1 By Cultured Human Amnion Cellsmentioning

confidence: 96%

“…A variety of non-vascular cells including cultured human amnion cells were reported to secrete ET-1-LI [6,8,9]. ET-1-LI levels in human amnion tissue, obtained at the time of elective cesarean section at Fig.…”

Section: Secretion Of Et-1 By Cultured Human Amnion Cellsmentioning

confidence: 99%

“…ET-1 secretion from cultured human amnion cells was stimulated by cytokines such as IL-1/3, IL-6 and tumor necrosis factor-a (TNF-a) [7,9] and by growth factors such as epidermal growth factor (EGF) and TGF-/3 [36]. Fig.…”

Section: Regulation Of Et-1 Secretion From Cultured Amnion Cellsmentioning

confidence: 99%

“…peptides originally isolated from the culture medium of porcine aortic endothelial cells [1].Three distinct ET-related genes are found in the human genomic library,and the corresponding peptides are designated as ET-1,ET-2 and ET-3 [2].Prepro-ET-1 mRNA is constitutively expressed by cultured endothelial cells.ET-1 is also secreted from various non-vascular tissues including reproductive organs such as mammary tissue [3],decidual stromal cells of the maternal basal plate of the placenta [4],placental syncytiotrophoblasts and extravillous cytotrophoblasts [5]and amnion tissue [6][7][8][9].ET-2 and ET-3 are expressed in other tissues,such as the brain,kidney,adrenal,intestine and placenta [10][11][12].…”

mentioning

confidence: 99%

“…Additionally, they found that the ET-1-LI concentration in the upper compartment did not change after the onset of labor. On the other hand, ET-1-LI secretion by cultured amnion cells was stimulated by cytokines [7,9]. From these observations, they suggested that the increased ET-1-LI concentration in the forewaters was derived from amniotic tissue of the lower uterine segment stimulated by cytokines secreted from macrophages and/or leukocytes in the cervix and vagina [351.…”

mentioning

confidence: 99%

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Secretion of Endothelin by Avascular Amnion Tissue: Possible Roles in H Pregnancy

et al. 1994

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Abstract.Endothelin(ET)wasoriginally isolated from culture medium of porcine aortic endothelial cells as a potent vasoconstrictor peptide.Subsequently,ET was also reported to be produced by nonvascular tissues and to be involved in various biological phenomena in these tissues.Recently,a high concentration of ET-1-like immunoreactivity(ET-1-LI)was found in human amniotic fluid.Amnion tissue also contained a large amount of ET-1-LI,and cultured amnion cells secreted large amounts of ET-1-LI.The major component of ET-1-LI in these samples was ET-1.Moreover,the expression of prepro-ET-1 mRNA was detected in both amnion tissue and cultured amnion cells,indicating that ET-1 in the amniotic fluid originated from amnion cells.In cultured amnion cells,ET-1 secretion was stimulated by EGF,TGF-f3,IL-1a,13 and TNF-a.In addition,specific receptors for ET,ET-AR and ET-BR were detected in myometrium,decidua vera,chorion laeve,and placenta at term by both ligand binding and Northern blot analysis.These findings suggest that ET-1 secreted from amnion cells plays a physiological role in human pregnancy.In this paper,the regulation of production of ET-1 and expression of receptors for ET-1 by avascular human amnion tissue are reviewed.The possible importance of amniotic ET in human pregnancy is also discussed.

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