Endothelin (ET)-1 and ET-3 inhibit estrogen and cAMP production by rat granulosa cells in vitro

Calogero, A. E.; Burrello, Nunziatina; Ossino, A M

doi:10.1677/joe.0.1570209

Cited by 12 publications

(6 citation statements)

References 32 publications

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“…An increase in oestradiol and progesterone secretion after short term (2 h) incubation was noted in another study (Kubota et al, 1994) in human ovaries; however, those authors observed significant inhibition of FSH-and HCGstimulated accumulation of progesterone during 48 h incubation. Long term exposure to ET-1 and ET-3 led also to the inhibition of oestrogen and cAMP production in rat granulosa cells (Calogero et al, 1998). On the other hand, oestradiol treatment was found to decrease ET-1 concentration in peripheral blood (Polderman et al, 1993), but had no influence on specific ET-1 binding by rat granulosa cells (Otani et al, 1996).…”

Section: Discussionmentioning

confidence: 88%

Correlation between 21 amino acid endothelin, intrafollicular steroids and follicle size in stimulated cycles

et al. 1999

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Several in-vitro studies have shown that endothelins (ET) may inhibit synthesis of progesterone and prevent luteinization of granulosa cells. In the present study, a specific radioimmunoassay was used to evaluate the correlation between concentrations of active (21 residue) ET and ovarian steroids in 47 samples of human follicular fluid (FF) following gonadotrophin stimulation for in-vitro fertilization (IVF) protocols. An isoform non-selective antibody was used in the radioimmunoassay, which recognized the C-terminal structure of the 21 residue ET, and therefore did not crossreact with their weakly active precursors - big ET. In pooled samples of follicular fluid (FF), the concentration of 21 amino acid ET correlated negatively with diameter of the follicles (r = -0.31, P < 0.05) and progesterone concentrations in FF (r = -0.56, P < 0. 001). A positive relationship (non-significant) was found between ET and testosterone concentrations. No correlation between ET and oestradiol was observed. The within-patient correlation coefficients were also evaluated in women from whom three or more samples of FF were obtained. ET were markedly inversely correlated with follicle size in all cases, and with progesterone in five of seven women. Five of seven patients also showed significant positive correlation of ET with testosterone. The results demonstrate clinical evidence that active ET play an important role in regulation of follicle development, especially in the inhibition of premature luteinization of granulosa cells.

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Section: Discussionmentioning

confidence: 88%

Correlation between 21 amino acid endothelin, intrafollicular steroids and follicle size in stimulated cycles

et al. 1999

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“…The prevailing notion is that this peptide acts to prevent premature luteinization of follicular cells. However, ET-1 does not selectively inhibit progesterone production; ET-1 inhibited gonadotropin-induced changes in granulosa cell morphology [45] and cAMP and estradiol production by these cells [46,47]. Therefore, the physiological role of ET-1 in follicles needs further research.…”

Section: Ece-1 Was Expressed In Bovine Follicles and CL Wherementioning

confidence: 99%

Hormonal Regulation and Cell-Specific Expression of Endothelin-Converting Enzyme 1 Isoforms in Bovine Ovarian Endothelial and Steroidogenic Cells1

Levy¹,

Gordin²,

Smith³

et al. 2003

Biology of Reproduction

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Endothelin-converting enzyme 1 (ECE-1) is a key enzyme in the biosynthesis of endothelin 1 (ET-1), a potent regulator of ovarian function. Different ECE-1 isoforms are localized in distinct intracellular compartments. Thus, the spatial and temporal pattern of ECE-1 expression determines the site of big ET-1 activation and the bioavailability of ET-1. This study was undertaken to investigate the hormonal regulation and cell-specific expression of ECE-1 isoforms in endothelial and steroidogenic cells of bovine follicles and corpora lutea (CL). Using enriched follicular and luteal cell subpopulations and in situ hybridization techniques, we showed that the ECE-1 gene is expressed by both endothelial and steroidogenic cells; however, the intracellular ECE-1a isoform was present only in ET-1-expressing endothelial cells. Steroidogenic cells in follicles or in CL, deficient in ET-1, expressed only the plasma membrane ECE-1b isoform. The intensity of antisense ECE-1 labeling in the granulosa cell layer increased with follicular size; insulin-like growth factor I and insulin upregulated ECE-1 expression when cultured with granulosa cells, suggesting that these growth factors may increase ECE-1 in growing follicles. In contrast, ET-1 and LH downregulated ECE-1 in steroidogenic cells. This effect could account for low ECE (and ET-1) levels, which characterize the early luteal phase. These findings suggest that ECE-1 is regulated during different stages of the cycle in a physiologically relevant manner. The hormonal regulation and intracellular localization of bovine ECE-1 isoforms revealed in this study may provide new insights into ET-1 biosynthesis and mode of action in different cellular microenvironments within the ovary.

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“…The EDN3 gene has been reported to influence the function of granulosa cells in several mammals. This can significantly suppress estrogen production induced by follicular-stimulating hormone (FSH) (Calogero et al, 1998; Ervin et al, 2017). The expression of EDN3 can be regulated by gonadotropins in epithelial cells of the oviduct, particularly increased in the isthmus of this tissue (Jeoung et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Genomic Analysis Reveals Pleiotropic Alleles at EDN3 and BMP7 Involved in Chicken Comb Color and Egg Production

Dong

Zhang

et al. 2019

Front. Genet.

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Artificial selection is often associated with numerous changes in seemingly unrelated phenotypic traits. The genetic mechanisms of correlated phenotypes probably involve pleiotropy or linkage of genes related to such phenotypes. Dongxiang blue-shelled chicken, an indigenous chicken breed of China, has segregated significantly for the dermal hyperpigmentation phenotype. Two lines of the chicken have been divergently selected with respect to comb color for over 20 generations. The red comb line chicken produces significantly higher number of eggs than the dark comb line chicken. The objective of this study was to explore potential mechanisms involved in the relationship between comb color and egg production among chickens. Based on the genome-wide association study results, we identified a genomic region on chromosome 20 involving EDN3 and BMP7 , which is associated with hyperpigmentation of chicken comb. Further analyses by selection signatures in the two divergent lines revealed that several candidate genes, including EDN3 , BMP7 , BPIFB3 , and PCK1 , closely located on chromosome 20 are involved in the development of neural crest cell and reproductive system. The two genes EDN3 and BMP7 have known roles in regulating both ovarian function and melanogenesis, indicating the pleiotropic effect on hyperpigmentation and egg production in blue-shelled chickens. Association analysis for egg production confirmed the pleiotropic effect of selected loci identified by selection signatures. The study provides insights into phenotypic evolution due to genetic variation across the genome. The information might be useful in the current breeding efforts to develop improved breeds for egg production.

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Endothelin (ET)-1 and ET-3 inhibit estrogen and cAMP production by rat granulosa cells in vitro

Cited by 12 publications

References 32 publications

Correlation between 21 amino acid endothelin, intrafollicular steroids and follicle size in stimulated cycles

Correlation between 21 amino acid endothelin, intrafollicular steroids and follicle size in stimulated cycles

Hormonal Regulation and Cell-Specific Expression of Endothelin-Converting Enzyme 1 Isoforms in Bovine Ovarian Endothelial and Steroidogenic Cells1

Genomic Analysis Reveals Pleiotropic Alleles at EDN3 and BMP7 Involved in Chicken Comb Color and Egg Production

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