Endothelin-Converting Enzyme 1 and Vascular Endothelial Growth Factor as Potential Biomarkers during Ex Vivo Lung Perfusion with Prolonged Hypothermic Lung-Sparing

Hernández-Jiménez, Claudia; Olmos-Zuñiga, J. Raúl; Baltazares-Lipp, Matilde; Jasso-Victoria, Rogelio; Polo-Jerez, Adrián; Pérez-López, María; Vázquez-Justiniano, Luis Florentino; Díaz-Martínez, N.E.; Gaxiola-Gaxiola, Miguel; Romero-Romero, Laura; Guzmán-Cedillo, Axel Edmundo; Baltazares-Lipp, Mario Enrique; Vázquez-Minero, Juan Carlos; Gutiérrez‐González, Luis H.; Alonso-Gómez, Marcelino; Silva-Martínez, Mariana

doi:10.1155/2022/6412238

Cited by 3 publications

(1 citation statement)

References 38 publications

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“…Its malfunction within the walls of major arteries is an important contributor to the local and systemic signs of poor endothelial functions in health. However, in sickness, it promotes the normal functioning of many tissues and organs of the body (Hernández‐Jiménez et al, 2022; Sanusi et al, 2020). Increased oxidative/nitrosative stress, up‐regulation of inflammatory activity, and, most importantly, defective endothelin/endothelial nitric oxide synthase (eNOS) signaling have all been documented as precursors of endothelial dysfunction (Asiwe, Kolawole, et al, 2022; Jasińska‐Stroschein, 2022; Virk et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Treatment with Ginkgo biloba supplement modulates oxidative disturbances, inflammation and vascular functions in oxygen deprived hypothyroid mice: Involvement of endothelin‐1/NO signaling pathways

Adebayo¹,

Aduema

Iwueke

et al. 2022

Journal of Food Biochemistry

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A double‐hit biological alteration involving exposure to oxygen deprivation in hypothyroid condition may exacerbate cellular oxidative and inflammatory disturbances comparative to a one‐hit biological exposure. This study investigated the therapeutic effect of Ginkgo biloba as cardioprotective against aortic oxido‐inflammatory disturbances following oxygen deprivation in hypothyroid mice. Male Swiss mice were partitioned into 5 groups (n = 6) for hypothyroidism (Carbimazole 1.2 mg/kg) and hypoxia induction. Group 1 (normal control), group 2 (hypoxic stress control), group 3 (hypoxic and hypothyroid stress), group 4 (hypoxic and hypothyroid stress and Ginkgo biloba 20 mg/kg; p.o) and group 5 (hypoxic and hypothyroid stress and Levothyroxine 10 μg/kg; p.o) for 14 days. Thereafter, serum and aorta was collected for biochemical evaluation. GBS did not up‐regulate the serum thyroid hormone imbalances (tri‐iodothyronine (T3), thyroxin (T4)) but maintains the TSH levels. The blood glucose level was reduced with decrease oxidative stress and inflammatory mediators in the serum/aorta indicated by inhibited redox status following treatment with GBS. Moreover, endothelin‐1/nitric oxide signaling pathways were markedly regulated in the aorta. Conclusively, GBS acts as a therapeutic agent and may be consider as a potential vasodilator candidate in the management and control of hypoxic stress in hypothyroid condition. Practical applications Treatment with Gingko biloba supplement abated endothelial abnormalities via elevation of nitric oxide release and suppression of endothelin activity in hypothyroid mice exposed to hypoxic hypoxia. The activity of myeloperoxidase enzyme and redo‐inflammatory status was downregulated following treatment with Gingko biloba supplement in hypothyroid mice exposed to hypoxic hypoxia. Treatment with Gingko biloba supplement modulates hypothalamic–pituitary–adrenal (HPA) axis by inhibiting corticosterone release in hypothyroid mice exposed to hypoxic hypoxia.

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Section: Introductionmentioning

confidence: 99%

Treatment with Ginkgo biloba supplement modulates oxidative disturbances, inflammation and vascular functions in oxygen deprived hypothyroid mice: Involvement of endothelin‐1/NO signaling pathways

Adebayo¹,

Aduema

Iwueke

et al. 2022

Journal of Food Biochemistry

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Exploring predisposing factors and pathogenesis contributing to injuries of donor lungs

Zhang

et al. 2022

Expert Review of Respiratory Medicine

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Changes in the levels of free sialic acid during ex vivo lung perfusion do not correlate with pulmonary function. Experimental model

Hernández-Jiménez,

Martínez-Cortés,

Olmos-Zuñiga

et al. 2023

BMC Pulm Med

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Background Ex vivo lung perfusion (EVLP) constitutes a tool with great research potential due to its advantages over in vivo and in vitro models. Despite its important contribution to lung reconditioning, this technique has the disadvantage of incurring high costs and can induce pulmonary endothelial injury through perfusion and ventilation. The pulmonary endothelium is made up of endothelial glycocalyx (EG), a coating of proteoglycans (PG) on the luminal surface. PGs are glycoproteins linked to terminal sialic acids (Sia) that can affect homeostasis with responses leading to edema formation. This study evaluated the effect of two ex vivo perfusion solutions on lung function and endothelial injury. Methods We divided ten landrace swine into two groups and subjected them to EVLP for 120 min: Group I (n = 5) was perfused with Steen® solution, and Group II (n = 5) was perfused with low-potassium dextran-albumin solution. Ventilatory mechanics, histology, gravimetry, and sialic acid concentrations were evaluated. Results Both groups showed changes in pulmonary vascular resistance and ventilatory mechanics (p < 0.05, Student’s t-test). In addition, the lung injury severity score was better in Group I than in Group II (p < 0.05, Mann–Whitney U); and both groups exhibited a significant increase in Sia concentrations in the perfusate (p < 0.05 t-Student) and Sia immunohistochemical expression. Conclusions Sia, as a product of EG disruption during EVLP, was found in all samples obtained in the system; however, the changes in its concentration showed no apparent correlation with lung function.

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Endothelin-Converting Enzyme 1 and Vascular Endothelial Growth Factor as Potential Biomarkers during Ex Vivo Lung Perfusion with Prolonged Hypothermic Lung-Sparing

Cited by 3 publications

References 38 publications

Treatment with Ginkgo biloba supplement modulates oxidative disturbances, inflammation and vascular functions in oxygen deprived hypothyroid mice: Involvement of endothelin‐1/NO signaling pathways

Treatment with Ginkgo biloba supplement modulates oxidative disturbances, inflammation and vascular functions in oxygen deprived hypothyroid mice: Involvement of endothelin‐1/NO signaling pathways

Exploring predisposing factors and pathogenesis contributing to injuries of donor lungs

Changes in the levels of free sialic acid during ex vivo lung perfusion do not correlate with pulmonary function. Experimental model

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