Endothelin causes contraction of human esophageal muscularis mucosae through interaction with both ETA and ETB receptors

Huang, Shih-Che; Chang, Bee-Song

doi:10.1016/j.regpep.2003.10.016

Cited by 8 publications

(7 citation statements)

References 32 publications

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“…A similar interaction of ET receptors has been described in a number of tissues, being first described in the vasculature in 1995 (3,32,46,49,67,69,78,89,102). At the base of this interaction is the failure of ET A receptor antagonists to shift venous contraction to ET-1, unless ET B receptor function is ablated.…”

Section: Et a /Et B Receptor Heterodimerizationmentioning

confidence: 62%

Endothelin receptors: what's new and what do we need to know?

Watts

2010

American Journal of Physiology-Regulatory, Integrative and Comp

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Receptors are at the heart of how a molecule transmits a signal to a cell. Two receptor classes for endothelin (ET) are recognized, the ET(A) and ET(B) receptors. Intriguing questions have arisen in the field of ET receptor pharmacology, physiology, and function. For example, a host of pharmacological studies support the interaction of the ET(A) and ET(B) receptor in tissues (veins, arteries, bronchus, arterioles, esophagus), but yet few have been able to demonstrate direct ET(A)/ET(B) receptor interaction. Have we modeled this interaction wrong? Do we have a truly selective ET(A) receptor agonist such that we could selectively stimulate this important receptor? What can we learn from the recent phylogenic studies of the ET receptor family? Have we adequately addressed the number of biological molecules with which ET can interact to exert a biological effect? Recent mass spectrometry studies in our laboratory suggest that ET-1 interacts with other hereto unrecognized proteins. Biased ligands (ligands at the same receptor that elicit distinct signaling responses) have been discovered for other receptors. Do these exist for ET receptors and can we take advantage of this possibility in drug design? These and other questions will be posed in this minireview on topics on ET receptors.

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Section: Et a /Et B Receptor Heterodimerizationmentioning

confidence: 62%

Endothelin receptors: what's new and what do we need to know?

Watts

2010

American Journal of Physiology-Regulatory, Integrative and Comp

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“…20,21 Measurement of contraction of isolated human oesophageal muscularis mucosae Measurements of contraction of isolated muscularis mucosae strips from the human oesophagus were performed according to the procedure published previously. [20][21][22] In brief, the oesophagus tissue obtained from surgery was placed in standard incubation solution, containing 118 mmol L )1 NaCl, 25 mmol L )1 NaHCO 3 , 4.7 mmol L )1 KCl, 14 mmol L )1 glucose, 1.2 mmol L )1 NaH 2-PO 4 , 1.8 mmol L )1 CaCl 2 , gassed with 95% O 2 -5% CO 2 . The final pH at 37°C was 7.40 ± 0.05.…”

Section: Methodsmentioning

confidence: 99%

“…The strips were connected to an isometric transducer (FT.03; Grass Technologies, West Warwick, RI, USA), which was connected to an amplifier (Gould Instrument Systems, Valley View, OH, USA) and a computer recording system (BIOPAC systems, Goleta, CA, USA). The basal tension of the muscle strips was adjusted to 1.0 g. 20,21 Experiments were started after a 45 min equilibration period. Carbachol (1 lmol L )1 )-induced contraction was used as a reference to express contractile response to agonists.…”

Section: Methodsmentioning

confidence: 99%

“…For studies using atropine and tetrodotoxin, the muscle strips were exposed to the indicated concentrations of these agents for 6 and 15 min, respectively, and then to the various concentrations of peptides or proteinases. [20][21][22] One cumulative concentration-response curve, with or without tetrodotoxin or atropine, was constructed with each preparation.…”

Section: Methodsmentioning

confidence: 99%

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Proteinase‐activated receptors 1and 2 mediate contraction of human oesophageal muscularis mucosae

Chang

Huang

2009

Neurogastroenterology Motil

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Proteinase-activated receptors 1 and 2 mediate contraction of the human gallbladder. In the present study, we investigated effects mediated by proteinase-activated receptors (PARs) in the human oesophagus by measuring contraction of muscularis mucosae strips isolated from the human oesophagus. Both PAR(1) agonists (thrombin, SFLLRN-NH(2) and TFLLR-NH(2)) and PAR(2) agonists (trypsin, 2-furoyl-LIGRLO-NH(2) and SLIGKV-NH(2)) caused concentration-dependent contraction. In contrast, PAR(1) and PAR(2) control peptides did not cause contraction. The existence of PAR(1) and PAR(2) in the human oesophageal muscularis mucosae was confirmed by immunohistochemistry and reverse transcription-polymerase chain reaction. On the other hand, PAR(4) agonists, GYPGKF-NH(2), GYPGQV-NH(2) and AYPGKF-NH(2), did not cause contraction or relaxation in resting or carbachol-contracted muscularis mucosae strips, suggesting that PAR(4) is not involved in human oesophageal motility. The contractile responses to SFLLRN-NH(2) and trypsin in the human oesophagus were insensitive to atropine and tetrodotoxin, indicating that the contractile response was not neurally mediated. Taken together, these results demonstrate that PAR(1) and PAR(2) but not PAR(4) mediate contraction in human oesophageal muscularis mucosae. PAR(1) and PAR(2) may influence human oesophageal motility.

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“…Measurements of contraction of isolated muscularis mucosae strips from the human and guinea-pig oesophagus were performed according to the procedure published previously. [26][27][28] In brief, the guinea pig was killed and the oesophagus removed, cut longitudinally and washed. The guinea-pig oesophagus or human oesophagus, obtained from surgery, was placed in standard incubation solution, containing 118 mmol L )1 NaCl, 25 mmol L )1 NaHCO 3 , 4.7 mmol L )1 KCl, 14 mmol L )1 glucose, 1.2 mmol L )1 NaH 2 PO 4 , 1.8 mmol L )1 CaCl 2 , gassed with 95% O 2 -5% CO 2 .…”

Section: Measurement Of Contraction Of Isolated Human and Guinea-pig mentioning

confidence: 99%

Cysteinyl leucotriene receptor type 1 mediates contraction in human and guinea‐pig oesophagus

Chang

Wang

et al. 2008

Neurogastroenterology Motil

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Leucotriene D(4) (LTD(4)) causes contraction of the guinea-pig and cat oesophagus. Effects of cysteinyl leucotrienes in the human oesophagus were unknown. To investigate and compare the cysteinyl leucotriene effects in the human oesophagus with those in the guinea-pig oesophagus, we measured contraction of muscularis mucosae strips isolated from the human and guinea-pig oesophagus caused by cysteinyl leucotrienes, LTC(4), LTD(4) and LTE(4), as well as the dihydroxy leucotriene, LTB(4). Effects of leucotrienes in human were similar to those in guinea-pig oesophagus. LTC(4) and LTD(4) caused moderate, whereas LTE(4) caused mild, concentration-dependent contraction. LTE(4) was a partial agonist. In contrast, LTB(4) did not cause any contraction. The relative potencies for cysteinyl leucotrienes to cause contraction were LTD(4) = LTC(4) > LTE(4). The LTD(4)-induced contraction was moderately inhibited by two selective CysLT(1) receptor antagonists, montelukast and zafirlukast, in both human and guinea-pig oesophagus. In addition, the LTD(4)-induced contraction was not and only slightly inhibited by BAY u9773, the CysLT(1) and CysLT(2) receptor antagonist, in the human and guinea-pig oesophageal muscularis mucosae respectively. These indicate the existence of the CysLT(1) mediating oesophageal contraction in both human and guinea-pig oesophagus. The LTD(4)-induced contraction was not affected by tetrodotoxin, atropine or capsaicin, suggesting a direct effect. These results demonstrate that cysteinyl leucotrienes but not the dihydroxy leucotriene cause contraction in the human and guinea-pig oesophagus. CysLT(1) mediates contraction in both human and guinea-pig oesophagus.

show abstract

Endothelin causes contraction of human esophageal muscularis mucosae through interaction with both ETA and ETB receptors

Cited by 8 publications

References 32 publications

Endothelin receptors: what's new and what do we need to know?

Endothelin receptors: what's new and what do we need to know?

Proteinase‐activated receptors 1and 2 mediate contraction of human oesophageal muscularis mucosae

Cysteinyl leucotriene receptor type 1 mediates contraction in human and guinea‐pig oesophagus

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