Endothelin-1 stimulates heat shock protein 27 induction in osteoblasts: involvement of p38 MAP kinase

Kawamura, Hidenori; Kozawa, Osamu; Matsuno, Hiroyuki; Niwa, Masayuki; Otsuka, Takanobu; Matsui, Nobuo; Kato, Kanefusa

doi:10.1016/s0021-5198(19)48034-7

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“…HSP27 functions through a process that is phosphorylated by the action of various kinases (pHSP27). In osteoblasts, TGF-β1 (transforming growth factor beta 1) is phosphorylated by the action of hydrogen peroxide [ 26 ] or by p38MAPK (p38MAPkinase) [ 27 ]. p38 MAPK was phosphorylated by HSP27 and is thought to be working with HSP27 necessary for the protection of various proteins formed from 140-800 kDa oligomers [ 28 ], preventing apoptosis during early stages and cell differentiation [ 29 - 31 ].…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical expression of heat shock proteins in the mouse periodontal tissues due to orthodontic mechanical stress*

et al. 2010

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The histopathology of periodontal ligament of the mouse subjected to mechanical stress was studied. Immunohistochemical expressions of HSP27 and pHSP27 were examined. Experimental animals using the maxillary molars of ddY mouse by Waldo method were used in the study. A separator was inserted to induce mechanical stress. After 10 minutes, 20 minutes, 1 hour, 3 hours, 9 hours and 24 hours, the regional tissues were extracted, fixed in 4% paraformaldehyde and 0.05 M phosphate-buffered fixative solution. Paraffin sections were made for immunohistochemistry using HSP27 and p-HSP27. In the control group, the periodontal ligament fibroblasts expressed low HSP27 and p-HSP27. However, in the experimental group, periodontal ligament fibroblasts expressed HSP27 10 minutes after mechanical load application in the tension side. The strongest expression was detected 9 hours after inducing mechanical load. p-HSP27 was also expressed in a time-dependent manner though weaker than HSP27. The findings suggest that HSP27 and p-HSP27 were expressed for the maintenance of homeostasis of periodontal ligament by the activation of periodontal ligament fibroblasts on the tension side. It also suggests that these proteins act as molecular chaperones for osteoblast activation and maintenance of homeostasis.

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