Endothelin-1 mRNA is widely expressed in porcine and human tissues.

Abstract

Endothelin-1 (ET-1) is a potent vasoconstrictor peptide isolated from porcine endothelial cells. We have previously demonstrated widespread binding sites for ET-1 in blood vessels, heart, kidney, adrenal, lung, and brain in a distribution that paralleled that of endothelial cells. To determine whether these cells are capable of synthesizing ET-1 in close proximity to its binding sites, amplification of cDNA using the polymerase chain reaction and in situ hybridization were used to study the distribution of ET-1 mRNA. We have found widespread transcription of ET-1 mRNA in human and porcine tissues. The identity of the transcripts was confirmed by prediction of restriction fragment lengths or sequencing. In situ hybridization in the kidney showed that the regional expression of these transcripts is localized, probably to small blood vessels, but the failure to visualize ET-1 mRNA in the capillaries may reflect absence of expression or insufficient sensitivity of the technique. These results should permit investigation of the role of ET-1 as a local factor in vascular pathophysiology. (J. Clin.

Gene expression of vasoactive intestinal contractor/endothelin-2 in ovary, uterus and embryo: comprehensive gene expression profiles of the endothelin ligand-receptor system revealed by semi-quantitative reverse transcription-polymerase chain reaction analysis in adult mouse tissues and during late embryonic development

“…ND, not detected. 1988, Bloch et al 1989, MacCumber et al 1989, Matsumoto et al 1989, Arai et al 1990, Nunez et al 1990, Sakurai et al 1990, Firth & Ratcliffe 1992, Shiba et al 1992. However, different analytical methods (Northern blot analysis, RNase protection assay, immunological detection methods, and RT-PCR), different species (rat, human, and pig), and different stages of animal development have been presented in these reports.…”

Ultraviolet B irradiation increases endothelin‐1 and endothelin receptor expression in cultured human keratinocytes

“…cDNA was generated using 1/lg of total RNA as a template in the presence of cloned murine Moloney leukemia virus reverse transcriptase and random hexamers. ET-1 primers were designed as 5'-TGC TCC TGC TCT TCC CTG ATG GAT AAA GAG TGT GTC-3" (sense) and 5'-GGT CAC ATA ACG CTC TCT GGA GGG CTT-3' (antisense) [27]. The primers were predicted to amplify a 462 base pair DNA fragment (nucleotides 157 to 618) [2].…”

Maturation of the expression of adrenomedullin, endothelin-1 and nitric oxide synthases in adipose tissues from childhood to adulthood

“…12 It is also expressed in various other types of cells and organs (such as blood vessels, heart, kidney, adrenal glands, lung and brain) and acts via two types of ET receptors, the ET-A and ET-B receptors, in a paracrine and autocrine manner. 13,14 ET-1 has proliferative properties in smooth muscle cells and also promotes endocrine and metabolic functions, such as aldosterone or cortisol secretion. 14,15 Additionally, it has been shown that ET-1 may stimulate leptin secretion in cultured adipocytes.…”