Endothelialization of Polycaprolactone Vascular Graft under the Action of Locally Applied Vascular Endothelial Growth Factor

Sevost'yanova, V. V.; Антонова, Л. В.; Великанова, Е. А.; Матвеева, В. Г.; Кривкина, Е. О.; Глушкова, Т. В.; Миронов, А. В.; Burago, A. Yu.; Ls, Barbarash

doi:10.1007/s10517-018-4144-4

Cited by 16 publications

(15 citation statements)

References 13 publications

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“…Among pro-endothelialization factors, VEGF is the most potent identified to date. 64 Our results were consistent with these findings. TiO 2 nanotube supernatants, compared with those of CP, contained more VEGF and promoted HUVEC proliferation, adhesion, migration, and tube formation.…”

supporting

confidence: 92%

Nanotubular TiO<sub>2</sub> regulates macrophage M2 polarization and increases macrophage secretion of VEGF to accelerate endothelialization via the ERK1/2 and PI3K/AKT pathways

Dong

Ding

et al. 2019

IJN

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BackgroundMacrophages play important roles in the immune response to, and successful implantation of, biomaterials. Titanium nanotubes are considered promising heart valve stent materials owing to their effects on modulation of macrophage behavior. However, the effects of nanotube-regulated macrophages on endothelial cells, which are essential for stent endothelialization, are unknown. Therefore, in this study we evaluated the inflammatory responses of endothelial cells to titanium nanotubes prepared at different voltages.Methods and resultsIn this study we used three different voltages (20, 40, and 60 V) to produce titania nanotubes with three different diameters by anodic oxidation. The state of macrophages on the samples was assessed, and the supernatants were collected as conditioned media (CM) to stimulate human umbilical vein endothelial cells (HUVECs), with pure titanium as a control group. The results indicated that titanium dioxide (TiO2) nanotubes induced macrophage polarization toward the anti-inflammatory M2 state and increased the expression of arginase-1, mannose receptor, and interleukin 10. Further mechanistic analysis revealed that M2 macrophage polarization controlled by the TiO2 nanotube surface activated the phosphatidylinositol 3-kinase/AKT and extracellular signal-regulated kinase 1/2 pathways through release of vascular endothelial growth factor to influence endothelialization.ConclusionOur findings expanded our understanding of the complex influence of nanotubes in implants and the macrophage inflammatory response. Furthermore, CM generated from culture on the TiO2 nanotube surface may represent an integrated research model for studying the interactions of two different cell types and may be a promising approach for accelerating stent endothelialization through immunoregulation.

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supporting

confidence: 92%

Nanotubular TiO<sub>2</sub> regulates macrophage M2 polarization and increases macrophage secretion of VEGF to accelerate endothelialization via the ERK1/2 and PI3K/AKT pathways

Dong

Ding

et al. 2019

IJN

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“…Explanted grafts were fixed in 10% neutral phosphate buffered formalin (BioVitrum, Moscow, Russia). Sample processing and histological staining was performed as described previously [9][10][11][12][13]. All grafts were stained for hematoxylin-eosin (H&E), Van Gieson, orsein, and alizarin red S.…”

Section: Histological Examinationmentioning

confidence: 99%

“…Such functionally active biodegradable vascular grafts potentiate the replacement of a polymer backbone with a new vessel [8][9][10]. Accumulating evidence from experimental studies shows that polymer grafts supplemented by bioactive molecules are beneficial over standard unmodified grafts [11,12].…”

Section: Introductionmentioning

confidence: 99%

A Brief Report on an Implantation of Small-Caliber Biodegradable Vascular Grafts in a Carotid Artery of the Sheep

et al. 2020

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The development of novel biodegradable vascular grafts of a small diameter (<6 mm) is an unmet clinical need for patients requiring arterial replacement. Here we performed a pre-clinical study of new small-caliber biodegradable vascular grafts using a sheep model of carotid artery implantation. The 4 mm diameter vascular grafts were manufactured using a mix of polyhydroxybutyrate/valerate and polycaprolactone supplemented with growth factors VEGF, bFGF and SDF-1α (PHBV/PCL-GFmix) and additionally modified by a polymer hydrogel coating with incorporation of drugs heparin and iloprost (PHBV/PCL-GFmixHep/Ilo). Animals with carotid artery autograft implantation and those implanted with clinically used GORE-TEX® grafts were used as control groups. We observed that 24 h following surgery, animals with carotid artery autograft implantation showed 87.5% patency, while all the PHBV/PCL-GFmix and GORE-TEX® grafts displayed thrombosis. PHBV/PCL-GFmixHep/Ilo grafts demonstrated 62.5% patency 24 h following surgery and it had remained at 50% 1 year post-operation. All the PHBV/PCL grafts completely degraded less than 1 year following surgery and were replaced by de novo vasculature without evidence of calcification. On the other hand, GORE-TEX® grafts displayed substantial amounts of calcium deposits throughout graft tissues. Thus, here we report a potential clinical usefulness of PHBV/PCL grafts upon their additional modification by growth factors and drugs to promote endothelialization and reduce thrombogenicity.

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“…Благодаря VEGF на внутренней поверхности трансплантатов уже через месяц имплантации было идентифицировано большое количество незрелых CD31 + CD34 + эндотелиальных клеток, которые в динамике к окончанию срока имплантации в брюшную часть аорты крыс образовывали монослой с преобладанием зрелых клеток с фенотипом CD31 + CD34 -. Немодифицированные PCL-графты не были столь успешны [56]. Схожие результаты получены с сополимерными PHBV/PCL-графтами, модифицированными тем же ростовым фактором [57].…”

Section: основные биологически активные вещества используемые для моunclassified

Biodegradable small-diameter vascular graft: types of modification with bioactive molecules and RGD peptides

Senokosova

Кривкина

Антонова

et al. 2020

RJTAO

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The need for small-diameter grafts for replacing the damaged area of the blood pool is still very high. These grafts are very popular for coronary artery bypass grafting. Polymeric synthetic grafts are an alternative to autografts. A promising area of tissue engineering is the creation of a biodegradable graft. It can serve as the basis for de novo generation of vascular tissue directly in the patient’s body. Optimization of the polymer composition of products has led to improved physicomechanical and biocompatible properties of the products. However, the improvements are still far from needed. One of the decisive factors in the reliability of a small-diameter vascular graft is the early formation of endothelial lining on its inner surface, which can provide atrombogenic effect and full lumen of the future newly formed vessel. To achieve this goal, grafts are modified by incorporating bioactive molecules or functionally active peptide sequences into the polymer composition or immobilizing on its inner surface. Peptide sequences include cell adhesion site – arginine-glycine-aspartic acid (RGD peptide). This sequence is present in most extracellular matrix proteins and has a tropism for integrin receptors of endothelial cells. Many studies have shown that imitation of the functional activity of the natural extracellular matrix can promote spontaneous endothelization of the inner surface of a vascular graft. Moreover, configuration of the RGD peptide determines the survival and differentiation of endothelial cells. The linker through which the peptide is crosslinked to the polymer surface determines the bioavailability of the RGD peptide for endothelial cells.

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Endothelialization of Polycaprolactone Vascular Graft under the Action of Locally Applied Vascular Endothelial Growth Factor

Cited by 16 publications

References 13 publications

Nanotubular TiO<sub>2</sub> regulates macrophage M2 polarization and increases macrophage secretion of VEGF to accelerate endothelialization via the ERK1/2 and PI3K/AKT pathways

Nanotubular TiO<sub>2</sub> regulates macrophage M2 polarization and increases macrophage secretion of VEGF to accelerate endothelialization via the ERK1/2 and PI3K/AKT pathways

A Brief Report on an Implantation of Small-Caliber Biodegradable Vascular Grafts in a Carotid Artery of the Sheep

Biodegradable small-diameter vascular graft: types of modification with bioactive molecules and RGD peptides

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