Endothelial targeting of polymeric nanoparticles stably labeled with the PET imaging radioisotope iodine-124

Simone, Eric; Zern, Blaine J.; Chacko, Ann‐Marie; Mikitsh, John L.; Blankemeyer, Eric; Muro, Silvia; Stan, Radu V.; Muzykantov, Vladimir R.

doi:10.1016/j.biomaterials.2012.04.036

Cited by 72 publications

(77 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Radiolabeling with 125 I occurred readily on the SHPP-modified IONPs by electrophilic aromatic substitution [14–15]. Radiolabeling yields of ~25% were achievable for [ 125 I]IONP 50 , and IONP 100 .…”

Section: Resultsmentioning

confidence: 99%

Assessment of Global Cardiac Uptake of Radiolabeled Iron Oxide Nanoparticles in Apolipoprotein-E-Deficient Mice: Implications for Imaging Cardiovascular Inflammation

et al. 2013

Self Cite

View full text Add to dashboard Cite

Purpose Atherosclerosis is a leading cause of death in industrialized countries and is characterized by the accumulation of lipids and inflammatory cells, including macrophages, in blood vessel walls. Therefore, the ability to image macrophages could help identify plaques that are precursors of acute thrombotic events. Previous research has shown that long-circulating, nanoparticles could be used to detect macrophages within atherosclerotic plaques of the aorta. By conducting this study, we investigated whether global cardiac uptake of radiolabeled nanoparticles could allow assessment of total macrophage burden in the coronary arteries. Procedures Dextran-coated Iron Oxide Nanoparticles (IONPs) were labeled with iodine-125 via Bolton-Hunter (SHPP) method. IONPs were characterized by means of dynamic light scattering and transmission electronic microscopy. Biodistribution studies were performed in healthy and atherosclerotic mice. Additionally, digital autoradiography of hearts from both healthy and atherosclerotic mice was performed to assess regional and global atherosclerotic burden. Results The [125I]IONPs exhibited high radiolabel stability and long blood circulation, which eventually led to high heart uptake in apoE −/− mice when compared with healthy controls. Furthermore, digital autoradiography showed substantially enhanced emission of signals from the hearts of atherosclerotic mice, while no or minimal cardiac signals were detected in healthy mice. Conclusions This preparation showed adequate physical-chemical properties for in vivo studies, such as small size (~30 nm), good radiolabel stability, and long circulation time. There was also significant accumulation in the heart of apoE−/− mice compared with that of healthy control animals. These findings suggest that radiolabeled dextran-coated iron oxide nanoparticles may have potential to become a useful tool to detect macrophages in the atherosclerosis plaques of coronary arteries; however, these preliminary findings should be confirmed by further studies in a larger scale in various atherosclerosis models.

show abstract

Section: Resultsmentioning

confidence: 99%

Assessment of Global Cardiac Uptake of Radiolabeled Iron Oxide Nanoparticles in Apolipoprotein-E-Deficient Mice: Implications for Imaging Cardiovascular Inflammation

et al. 2013

Self Cite

View full text Add to dashboard Cite

show abstract

“…To label the nanoparticles for radioactive detection with a gamma counter, a previously published method was used where the nanoparticles were incubated with I 125 and iodobeads to attach the I 125 to the phenol groups of the nanoparticles through the electrophilic aromatic substitution wherein the iodione is inserted in the ortho position to the hydroxyl group of the phenol molecule (Figure 4A) [40]. The I 125 substitution allows for detection in animal models via gamma counters and I 124 incorporation can be used for the detection of beta emitters using positron emission tomography (PET) or single photon emission computer tomography (SPECT) imaging [40]. For these studies, we focused on the immobilization of I 125 on the particles in order to enable the detection is specific organs using gamma counters.…”

Section: Discussionmentioning

confidence: 99%

Long-circulating Janus nanoparticles made by electrohydrodynamic co-jetting for systemic drug delivery applications

Rahmani

Villa

Dishman

et al. 2015

Journal of Drug Targeting

Self Cite

View full text Add to dashboard Cite

Background Nanoparticles with controlled physical properties have been widely used for controlled release applications. In addition to shape, the anisotropic nature of the particles can be an important design criterion to ensure selective surface modification or independent release of combinations of drugs. Purpose Electrohydrodynamic (EHD) co-jetting is used for the fabrication of uniform anisotropic nanoparticles with individual compartments and initial physicochemical and biological characterization is reported. Methods EHD co-jetting is used to create nanoparticles, which are characterized at each stage with scanning electron microscopy (SEM), structured illumination microscopy (SIM), dynamic light scattering (DLS) and nanoparticle tracking analysis (NTA). Surface immobilization techniques are used to incorporate polyethylene glycol (PEG) and I125 radiolabels into the nanoparticles. Particles are injected in mice and the particle distribution after 1, 4 and 24 hours is assessed. Results and discussion Nanoparticles with an average diameter of 105.7 nm are prepared by EHD co-jetting. The particles contain functional chemical groups for further surface modification and radiolabeling. The density of PEG molecules attached to the surface of nanoparticles is determined to range between 0.02 and 6.04 ligands per square nanometer. A significant fraction of the nanoparticles (1.2% injected dose per mass of organ) circulates in the blood after 24 h. Conclusion EHD co-jetting is a versatile method for the fabrication of nanoparticles for drug delivery. Circulation of the nanoparticles for 24 h is a pre-requisite for subsequent studies to explore defined targeting of the nanoparticles to a specific anatomic site.

show abstract

“…Incorporating 124 I to the structure of nanoparticles retains their bio-functionality and enables PET imaging [60]. Animal studies using 124 I-labelled nanopolymers targeted to specific endothelial determinants aid in optimization of targeted drug delivery, and nanophosphors tagged with radiolabelled ( 124 I) RGD for tumor imaging, have shown promising results [61,62].…”

Section: Nanoparticlesmentioning

confidence: 98%

The role of iodine-124 positron emission tomography in molecular imaging

Mahajan

Divgi

2016

Clin Transl Imaging

View full text Add to dashboard Cite

Radioactive iodine has been, in various forms, the mainstay of Nuclear Medicine. Iodine-123 is the most widely used iodine isotope for single photon imaging. Iodine-125 continues to be used in diverse applications from in vitro radioassay to in vivo estimation of various pathophysiologic correlates. Iodine-131 ( 131 I), useful for imaging as well as therapy, has contributed more than any other radionuclide to the growth and sustenance of Nuclear Medicine. Positron emission tomography (PET) is an indispensable tool in current clinical practice, spurred by the rapid and increasing availability of radiopharmaceuticals for in vivo imaging. Most clinical PET imaging utilizes fluorine-18; there is a need for positron emitters with longer half-lives, suitable for imaging larger molecules of interest. Iodine-124 ( 124 I) has approximately 23 % positron emission; its 4-day half-life lends itself to sequential imaging, and its dosimetry is comparable to iodine-131. Iodine can be easily attached to a variety of molecules without alteration of physico-chemical or biologic properties. PET with 124 I-labeled molecules enables longitudinal in vivo assessment of their distribution; such pharmacokinetic and biodistribution information has considerable utility in oncologic and non-oncologic applications. We will provide a clinical perspective on the physical and chemical characteristics of 124 I, as the iodide, as well as radiolabeled to a wide variety of molecules of interest.Radioiodination is accomplished based on the chemical and biologic nature of the ligand to be studied, and issues of relevance will be highlighted. We will conclude by describing the current and potential future clinical applications of 124 I-based tracers used for molecular imaging in diagnosis and therapy.

show abstract

Endothelial targeting of polymeric nanoparticles stably labeled with the PET imaging radioisotope iodine-124

Cited by 72 publications

References 56 publications

Assessment of Global Cardiac Uptake of Radiolabeled Iron Oxide Nanoparticles in Apolipoprotein-E-Deficient Mice: Implications for Imaging Cardiovascular Inflammation

Assessment of Global Cardiac Uptake of Radiolabeled Iron Oxide Nanoparticles in Apolipoprotein-E-Deficient Mice: Implications for Imaging Cardiovascular Inflammation

Long-circulating Janus nanoparticles made by electrohydrodynamic co-jetting for systemic drug delivery applications

The role of iodine-124 positron emission tomography in molecular imaging

Contact Info

Product

Resources

About