Endothelial progenitor cells regenerate infracted myocardium with neovascularisation development

Abstract

We achieved possibility of isolation, characterization human umbilical cord blood endothelial progenitor cells (EPCs), examination potency of EPCs to form new blood vessels and differentiation into cardiomyoctes in canines with acute myocardial infarction (AMI). EPCs were separated and cultured from umbilical cord blood. Their phenotypes were confirmed by uptake of double stains dioctadecyl tetramethylindocarbocyanine-labeled acetylated LDL and FITC-labeled Ulex europaeus agglutinin 1 (DILDL-UEA-1). EPCs of cord blood were counted. Human VEGFR-2 and eNOS from the cultured EPCs were assessed by qPCR. Human EPCs was transplanted intramyocardially in canines with AMI. ECG and cardiac enzymes (CK-MB and Troponin I) were measured to assess severity of cellular damage. Histopathology was done to assess neovascularisation. Immunostaining was done to detect EPCs transdifferentiation into cardiomyocytes in peri-infarct cardiac tissue. qPCR for human genes (hVEGFR-2, and eNOS) was done to assess homing and angiogenic function of transplanted EPCs. Cultured human cord blood exhibited an increased number of EPCs and significant high expression of hVEGFR-2 and eNOS genes in the culture cells. Histopathology showed increased neovascularization and immunostaining showed presence of EPCs newly differentiated into cardiomyocyte-like cells. Our findings suggested that hEPCs can mediate angiogenesis and differentiate into cardiomyoctes in canines with AMI.

Blood Flow Restriction Exercise Attenuates the Exercise-Induced Endothelial Progenitor Cell Response in Healthy, Young Men

“…These cells are bone marrow-derived and can be mobilized in response to vascular injury or an inflammatory stimulus (Asahara et al, 1999; Shintani et al, 2001). Since 1997, there have been a plethora of studies reporting their vasculogenic, angiogenic and vascular repair properties (Abd El Aziz et al, 2015; Yu et al, 2016; Chilla et al, 2018; Kong et al, 2018). In vascular disease states and in advancing age, circulating EPC number and function are lower (Fadini et al, 2006; Thijssen et al, 2006; Xia et al, 2012;Liao et al, 2014).…”

Comparative Evaluation for Potential Differentiation of Endothelial Progenitor Cells and Mesenchymal Stem Cells into Endothelial-Like Cells

“…Histopathology showed increased neo-vascularization and immunostaining showed presence of EPCs newly differentiated into cardiomyocyte-like cells. These findings suggested that hEPCs can mediate angiogenesis and differentiate into cardiomyocytes in canines with AMI ( 16 ).…”

Chronic stress: a crucial promoter of cell apoptosis in atherosclerosis

“…15 Normal feeding is extraordinarily important for the first 4 weeks after surgery because it can help to form a fibrous cap and the lipid core, similar to the human atherosclerotic plaque. 16…”