Endothelial progenitor cell-derived extracellular vesicles protect from complement-mediated mesangial injury in experimental anti-Thy1.1 glomerulonephritis

Cantaluppi, Vincenzo; Medica, Davide; Mannari, Claudio; Stiaccini, Giulia; Figliolini, Federico; Dellepiane, Sergio; Quercia, Alessandro Domenico; Migliori, Massimiliano; Panichi, Vincenzo; Giovannini, Luca; Bruno, Stefania; Tetta, Ciro; Biancone, Luigi; Camussi, Giovanni

doi:10.1093/ndt/gfu364

Cited by 73 publications

(64 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…EVs demonstrated a protective effect in experimental Thy1.1 glomerulonephritis in rats induced by complement-mediated mesangial injury (Cantaluppi et al, 2015). Indeed, after iv injection, EVs derived from endothelial progenitor cells localize within injured glomeruli and inhibit mesangial cell activation, leucocyte infiltration and apoptosis.…”

Section: Therapeutic Application Of Evs In Renal Pathologymentioning

confidence: 99%

“…Moreover, EV treatment decreases proteinuria, increases serum complement hemolytic activity, ameliorates renal function, preserves podocyte marker synaptopodin and endothelial antigen RECA-1. EVs' beneficial effect on mesangial cells was due to the inhibition of anti-Thy1.1 antibody/complement-induced apoptosis and C5b-9/C3 mesangial cell deposition (Cantaluppi et al, 2015). …”

Section: Therapeutic Application Of Evs In Renal Pathologymentioning

confidence: 99%

See 1 more Smart Citation

Extracellular Vesicles in Renal Pathophysiology

Pomatto

Gai

Bussolati

et al. 2017

Front. Mol. Biosci.

Self Cite

View full text Add to dashboard Cite

Extracellular vesicles are a heterogeneous population of microparticles released by virtually all living cells which have been recently widely investigated in different biological fields. They are typically composed of two primary types (exosomes and microvesicles) and are recently commanding increasing attention as mediators of cellular signaling. Indeed, these vesicles can affect recipient cells by carrying and delivering complex cargos of biomolecules (including proteins, lipids and nucleic acids), protected from enzymatic degradation in the environment. Their importance has been demonstrated in the pathophysiology of several organs, in particular in kidney, where different cell types secrete extracellular vesicles that mediate their communication with downstream urinary tract cells. Over the past few years, evidence has been shown that vesicles participate in kidney development and normal physiology. Moreover, EVs are widely demonstrated to be implicated in cellular signaling during renal regenerative and pathological processes. Although many EV mechanisms are still poorly understood, in particular in kidney, the discovery of their role could help to shed light on renal biological processes which are so far elusive. Lastly, extracellular vesicles secreted by renal cells gather in urine, thus becoming a great resource for disease or recovery markers and a promising non-invasive diagnostic instrument for renal disease. In the present review, we discuss the most recent findings on the role of extracellular vesicles in renal physiopathology and their potential implication in diagnosis and therapy.

show abstract

Section: Therapeutic Application Of Evs In Renal Pathologymentioning

confidence: 99%

Section: Therapeutic Application Of Evs In Renal Pathologymentioning

confidence: 99%

Extracellular Vesicles in Renal Pathophysiology

Pomatto

Gai

Bussolati

et al. 2017

Front. Mol. Biosci.

Self Cite

View full text Add to dashboard Cite

show abstract

“…In this study, total microRNA depletion by Dicer knockdown and RNase digestion diminished the protective effects of EPC–derived microvesicles. In another study by Cantaluppi et al [24], EVs were injected intravenously to rats with anti-Thy1.1 glomerulonephritis and these EVs were shown to have protective effects by amelioration of mesangial injury caused by antibody and complement-mediated mechanisms.…”

Section: Basic Concepts About Epcsmentioning

confidence: 99%

Endothelial Progenitor Cells and Kidney Diseases

Özkök

Yildiz

2018

Kidney Blood Press Res

View full text Add to dashboard Cite

Endothelial progenitor cells (EPC) are bone marrow derived or tissue-resident cells that play major roles in the maintenance of vascular integrity and repair of endothelial damage. Although EPCs may be capable of directly engrafting and regenerating the endothelium, the most important effects of EPCs seem to be depended on paracrine effects. In recent studies, specific microvesicles and mRNAs have been found to mediate the pro-angiogenic and regenerative effects of EPCs on endothelium. EPC counts have important prognostic implications in cardiovascular diseases (CVD). Uremia and inflammation are associated with lower EPC counts which probably contribute to increased CVD risks in patients with chronic kidney disease. Beneficial effects of the EPC therapies have been shown in studies performed on different models of CVD and kidney diseases such as acute and chronic kidney diseases and glomerulonephritis. However, lack of a clear definition and specific marker of EPCs is the most important problem causing difficulties in interpretation of the results of the studies investigating EPCs.

show abstract

“…This systemic condition is defined by accumulation of various products of nitrogen metabolism, such as urea. Intravenous injection of the isolated nanovesicles to mesangiolytic glomerulonephritis induced rats leads to the formation of vesicles around the injured renal region specifically the glomeruli (Cantaluppi et al, 2015). There have been various studies on investigation of aforementioned nanostructures for this purpose.…”

Section: Therapeutic Effects Of Exosomes On Kidney Injurymentioning

confidence: 99%

“…These cells are known to induce tissue repair by the paracrine mechanism including the release of nanovesicles. Intravenous injection of the isolated nanovesicles to mesangiolytic glomerulonephritis induced rats leads to the formation of vesicles around the injured renal region specifically the glomeruli (Cantaluppi et al, 2015). They inhibited mesangial cell activation, leucocyte infiltration, and apoptosis, in addition to decreasing proteinuria and increasing serum complement hemolytic activity.…”

Section: Therapeutic Effects Of Exosomes On Kidney Injurymentioning

confidence: 99%

A new approach in stem cell research—Exosomes: Their mechanism of action via cellular pathways

Derkuş

Emregül

2017

Cell Biology International

View full text Add to dashboard Cite

Exosomes are nano-sized vesicles surrounded by a lipid membrane, which tend to be secreted toward extra-cellular environments. Despite being defined as vesicles involved in excretion of molecular wastes by Rose Johnstone in the 1970s, further studies revealed them to be effective in various biological processes such as cancer development, regulation of the immune system, intercellular communication, stem cell biology, and tissue/organ regeneration. Although many studies dealing with the role of exosomes in stem cell differentiation and the use of exosomes isolated from stem cells for the treatment of several diseases have been published, the involved mechanisms remain largely unknown. Further understanding of these mechanisms, which include the involved cellular pathways, may improve the use of exosomes in diagnostic and treatment methods, especially for those involving stem cells. Here, we describe some recent data describing the action mechanism of stem cell-derived exosomes focusing on the implicated cellular pathways, hoping to provide novel information that will be useful for cell biology scientists working in this field.

show abstract

Endothelial progenitor cell-derived extracellular vesicles protect from complement-mediated mesangial injury in experimental anti-Thy1.1 glomerulonephritis

Abstract: EVs derived from EPCs exert a protective effect in Thy1.1 glomerulonephritis by inhibition of antibody- and complement-mediated injury of mesangial cells.

Cited by 73 publications

References 37 publications

Extracellular Vesicles in Renal Pathophysiology

Extracellular Vesicles in Renal Pathophysiology

Endothelial Progenitor Cells and Kidney Diseases

A new approach in stem cell research—Exosomes: Their mechanism of action via cellular pathways

Contact Info

Product

Resources

About