Endothelial nitric oxide synthase immunoreactivity in early gestation and in trophoblastic disease.

Ariel, Ilana; Hochberg, Abraham; Shochina, Mara

doi:10.1136/jcp.51.6.427

Cited by 46 publications

(30 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In rodents, Saxena et al reported up-regulation of iNOS in implantation site [46] and Fuhrmann et al demonstrated the importance of the combination of iNOS and progesterone in implantation [47]. By contrast, Ariel et al identified eNOS immunoreactiviry in the human extravillous trophoblastic cells, suggesting the possible involvement of eNOS in human implantation [48]. Thus, the physiological role of iNOS as well as eNOS in the chorionic villous tissues in the first trimester is still obscure in humans [49].…”

Section: Discussionmentioning

confidence: 99%

Expression of Nitric Oxide Synthase Isoforms in the Human Placenta Is Not Altered by Labor

et al. 2003

View full text Add to dashboard Cite

Abstract. Nitric oxide has various biological activities including smooth muscle relaxation, anti-inflammatory activity, anti-coagulatory activity. As the human placenta is known to express nitric oxide synthases, this study investigated the possible effect of labor on the expression of endothelial nitric oxide synthase (eNOS) and inducible nitric oxide synthase (iNOS) in human placental tissues at term. Both eNOS and iNOS mRNA expression in placental tissues in labor were significantly higher than those in the amnion, chorion laeve, decidua vera and myometrium. The eNOS mRNA and protein expressions in placental tissues in labor (n = 12) were 1.6023 ± 0.1652 (eNOS/GAPDH, mean ± SEM) and 12.8 ± 1.3 arbitrary units (AU), respectively, which were similar to those not in labor (n = 10), 1.5806 ± 0.2042 (eNOS/GAPDH) and 11.4 ± 1.8 AU. The iNOS mRNA and protein expressions in the placental tissues in labor were 1.2831 ± 0.2436 (iNOS/GAPDH) and 10.7 ± 2.1 AU respectively, similar to those not in labor, 1.9254 ± 0.8004 (iNOS/GAPDH) and 13.3 ± 1.8 AU. The guanosine 3',5'-cyclic monophosphate (cGMP) cocentration in the placental tissues in labor was 23.6 ± 1.4 fmol/g wet tissue, similar to that not in labor, 26.1 ± 2.0 fmol/g wet tissue. These findings suggest that nitric oxide production in the human placenta is maintained during labor.

show abstract

Section: Discussionmentioning

confidence: 99%

Expression of Nitric Oxide Synthase Isoforms in the Human Placenta Is Not Altered by Labor

et al. 2003

View full text Add to dashboard Cite

show abstract

“…Endothelial NOS is expressed in the fetal placenta from early pregnancy in several species (Sladek et al 1997, Ariel et al 1998, Gagioti et al 2000, Al-Hijji et al 2003. NOS are recognized as regulators of implantation and pregnancy maintenance, and angiogenesis in the fetal and maternal placenta (Gagioti et al 2000, Maul et al 2003; however, the mechanism of NOS effects on these processes remains to be elucidated.…”

Section: Discussionmentioning

confidence: 99%

Placental development during early pregnancy in sheep: cell proliferation, global methylation, and angiogenesis in the fetal placenta

Grazul-Bilska¹,

Johnson²,

Borowicz³

et al. 2011

Reproduction

View full text Add to dashboard Cite

To characterize early fetal placental development, gravid uterine tissues were collected from pregnant ewes every other day from day 16 to 30 after mating. Determination of 1) cell proliferation was based on Ki67 protein immunodetection; 2) global methylation was based on 5-methyl-cytosine (5mC) expression and mRNA expression for DNA methyltransferases (DNMTs) 1, 3a, and 3b; and 3) vascular development was based on smooth muscle cell actin immunolocalization and on mRNA expression of several factors involved in the regulation of angiogenesis in fetal membranes (FMs). Throughout early pregnancy, the labeling index (proportion of proliferating cells) was very high (21%) and did not change. Expression of 5mC and mRNA for DNMT3b decreased, but mRNA for DNMT1 and 3a increased. Blood vessels were detected in FM on days 18-30 of pregnancy, and their number per tissue area did not change. The patterns of mRNA expression for placental growth factor, vascular endothelial growth factor, and their receptors FLT1 and KDR; angiopoietins 1 and 2 and their receptor TEK; endothelial nitric oxide synthase and the NO receptor GUCY13B; and hypoxia inducing factor 1 a changed in FM during early pregnancy. These data demonstrate high cellular proliferation rates, and changes in global methylation and mRNA expression of factors involved in the regulation of DNA methylation and angiogenesis in FM during early pregnancy. This description of cellular and molecular changes in FM during early pregnancy will provide the foundation for determining the basis of altered placental development in pregnancies compromised by environmental, genetic, or other factors.

show abstract

“…In humans there are scant data on the specific role of NO in embryo development, decidual transformation, and embryo implantation. However, indirect evidences confirm that, depending on its concentrations, NO can both inhibit or stimulate embryonic development and implantation (28,29).…”

Section: Discussionmentioning

confidence: 99%

Embryonic Production of Nitric Oxide and Its Role in Implantation: A Pilot Study

Battaglia

Ciotti

Notarangelo

et al. 2003

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose : To investigate the ability of human embryos to produce nitric oxide (NO) and correlate its production with embryo quality and pregnancy rate. Methods : Twenty-three women participated in the study and were submitted to controlled ovarian stimulation and intracytoplasmic sperm injection. Embryos were singularly cultured in medium microdrops of 50 µL and were replaced, by transcervical transfer, at the 2-to 6-cell stage. In the culture media of each embryo the NO production was assessed by monitoring the levels of its stable oxidation products (nitrites/nitrates). Results : All the 23 patients underwent embryo transfer. After microinjection 64 embryos were obtained. The mean number of transferred embryos was 2.61 ± 0.46 and the pregnancy rate was 26%. The mean nitrite/nitrate concentrations of culture medium of each embryo was significantly higher (5.88 ± 2.34 µmol/L) than in pure P-1 medium (0.81 ± 0.21 µmol/L; p < 0.001) demonstrating an embryonic secretion of NO. Comparing pregnant (7.34 ± 2.72 µmol/L) versus nonpregnant patients (5.53 ± 1.49 µmol/L; p = 0.022), the mean nitrite/nitrate concentrations were significantly higher. Furthermore, the best quality embryos of pregnant women produced significantly higher nitrite/nitrate concentrations than those of not pregnant patients. Conclusions : It seems that NO production in nidating embryos is increased and that it may be primarily associated with a better morphology and a better growth potential of developing embryos.

show abstract

Endothelial nitric oxide synthase immunoreactivity in early gestation and in trophoblastic disease.

Cited by 46 publications

References 20 publications

Expression of Nitric Oxide Synthase Isoforms in the Human Placenta Is Not Altered by Labor

Expression of Nitric Oxide Synthase Isoforms in the Human Placenta Is Not Altered by Labor

Placental development during early pregnancy in sheep: cell proliferation, global methylation, and angiogenesis in the fetal placenta

Embryonic Production of Nitric Oxide and Its Role in Implantation: A Pilot Study

Contact Info

Product

Resources

About