Endothelial Nitric Oxide Synthase Haplotypes Associated with Hypertension Do Not Predispose to Cardiac Hypertrophy

Vasconcellos, Vivian B.; Lacchini, Riccardo; Jacob-Ferreira, Anna L.; Sales, Maria Lilian; Ferreira-Sae, Maria C.; Schreiber, Roberto; Nadruz, Wilson; Tanus‐Santos, José Eduardo

doi:10.1089/dna.2009.0955

Cited by 25 publications

(10 citation statements)

References 32 publications

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“…Preparation of the reactions was performed according to the manufacturer's specifications for each sample: 1x of the master mix, 1x of TaqMan genotyping assay, and 50 ng of template DNA in 10 μ l final volume. Real-time PCR was performed on StepOnePlus equipment (Applied Biosystems, USA) and analyzed with the manufacturer's software [ 35 ].…”

Section: Methodsmentioning

confidence: 99%

Effect of Multicomponent Training on Blood Pressure, Nitric Oxide, Redox Status, and Physical Fitness in Older Adult Women: Influence of Endothelial Nitric Oxide Synthase (NOS3) Haplotypes

Trapé

Lizzi

Gonçalves

et al. 2017

Oxidative Medicine and Cellular Longevity

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The purpose of this study was to verify the influence of the genotype or haplotype (interaction) of the NOS3 polymorphisms [-786T>C, 894G>T (Glu298Asp), and intron 4b/a] on the response to multicomponent training (various capacities and motor skills) on blood pressure (BP), nitrite concentration, redox status, and physical fitness in older adult women. The sample consisted of 52 participants, who underwent body mass index and BP assessments. Physical fitness was evaluated by six-minute walk, elbow flexion, and sit and stand up tests. Plasma/blood samples were used to evaluate redox status, nitrite concentration, and genotyping. Associations were observed between isolated polymorphisms and the response of decreased systolic and diastolic BP and increased nitrite concentration and antioxidant activity. In the haplotype analysis, the group composed of ancestral alleles (H1) was the only one to present improvement in all variables studied (decrease in systolic and diastolic BP, improvement in nitrite concentration, redox status, and physical fitness), while the group composed of variant alleles (H8) only demonstrated improvement in some variables of redox status and physical fitness. These findings suggest that NOS3 polymorphisms and physical training are important interacting variables to consider in evaluating redox status, nitric oxide availability and production, and BP control.

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Section: Methodsmentioning

confidence: 99%

Effect of Multicomponent Training on Blood Pressure, Nitric Oxide, Redox Status, and Physical Fitness in Older Adult Women: Influence of Endothelial Nitric Oxide Synthase (NOS3) Haplotypes

Trapé

Lizzi

Gonçalves

et al. 2017

Oxidative Medicine and Cellular Longevity

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“…Nitric oxide (NO) generated from endothelial NO synthase (eNOS) is the critical biochemical that mediates the response to shear stress in cell culture and animal studies [8,9]. This is clinically supported by the association of hypertension and eNOS haplotypes in human studies [10]. In line with human data, chronic NOS inhibition in rat or eNOS gene deletion in mice results in significant blood pressure elevation [11,12].…”

Section: Introductionmentioning

confidence: 93%

Role of shear stress and stretch in vascular mechanobiology

Kassab

2011

J. R. Soc. Interface.

270

198

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Blood vessels are under constant mechanical loading from blood pressure and flow which cause internal stresses (endothelial shear stress and circumferential wall stress, respectively). The mechanical forces not only cause morphological changes of endothelium and blood vessel wall, but also trigger biochemical and biological events. There is considerable evidence that physiologic stresses and strains (stretch) exert vasoprotective roles via nitric oxide and provide a homeostatic oxidative balance. A perturbation of tissue stresses and strains can disturb biochemical homeostasis and lead to vascular remodelling and possible dysfunction (e.g. altered vasorelaxation, tone, stiffness, etc.). These distinct biological endpoints are caused by some common biochemical pathways. The focus of this brief review is to point out some possible commonalities in the molecular pathways in response to endothelial shear stress and circumferential wall stretch.

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“…The reaction was carried out using Custom Taqman allele discrimination assay (re-synthesis part number AH5I790, Thermo Fisher, United States) and JumpStart Ready Mix for Quantitative PCR (Sigma-Aldrich, United States). Preparation of the reactions was performed according to the manufacturer's specifications (Vasconcellos et al, 2010). The NOS3 SNP at the position 894G > T (Glu298Asp) (rs1799983) was analyzed by conventional PCR followed by electrophoresis, using the following primers-forward: 5 -CATGAGGCTCAG CCCCAGAAC-3 ; reverse: 5 -AGTCAATCCCTTTGG TGC TCAC-3 .…”

Section: Genotypingmentioning

confidence: 99%

NOS3 Polymorphisms Can Influence the Effect of Multicomponent Training on Blood Pressure, Nitrite Concentration and Physical Fitness in Prehypertensive and Hypertensive Older Adult Women

et al. 2021

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Associations of endothelial nitric oxide synthase (NOS3) polymorphisms with hypertension and response to exercise training in prehypertensive and hypertensive older adult women remain unclear. This study used a multicomponent program (various capacities and motor skills) in the physical training intervention. It analyzed the influence of NOS3 polymorphisms [−786T > C, 894G > T (Glu298Asp), and intron 4b/a] on the response of blood pressure (BP), nitrite concentration, and physical fitness in older adult women. Fifty-two participants aged between 50 and 80 underwent body mass index, BP, 6-min walk, elbow flexion, and sit and stand-up tests to assess physical fitness. The intervention duration was 12 weeks, twice a week, on non-consecutive days. Each session lasted 90 min, maintaining an intensity between 13 (moderate) and 15 (intense), controlled by the Subjective Effort Perception Scale. Plasma/blood samples were collected to assess nitrite concentration and genotyping. The statistical analysis included Fisher’s exact test and linear mixed-effects models. The multicomponent training’s positive effect was observed with a similar response in both prehypertensive and hypertensive groups. However, carriers of different genotypes demonstrated different responses to training: the decreases in systolic and diastolic BP and increases in nitrite expected from the physical training were smaller in variant genotype than ancestral genotype carriers, especially in the hypertensive group. At positions −786T > C and Glu298Asp, only the ancestral genotypes showed a decrease in diastolic BP (Δ% = −8.1, and Δ% = −6.5, respectively) and an increase on nitrite (Δ% = 19.1, and Δ% = 24.1, respectively) in the hypertensive group. Our results show that the benefits of a multicomponent training intervention seem to be genotype-dependent. It should be possible to consider genetic variants when selecting an exercise treatment intervention.

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Endothelial Nitric Oxide Synthase Haplotypes Associated with Hypertension Do Not Predispose to Cardiac Hypertrophy

Cited by 25 publications

References 32 publications

Effect of Multicomponent Training on Blood Pressure, Nitric Oxide, Redox Status, and Physical Fitness in Older Adult Women: Influence of Endothelial Nitric Oxide Synthase (NOS3) Haplotypes

Effect of Multicomponent Training on Blood Pressure, Nitric Oxide, Redox Status, and Physical Fitness in Older Adult Women: Influence of Endothelial Nitric Oxide Synthase (NOS3) Haplotypes

Role of shear stress and stretch in vascular mechanobiology

NOS3 Polymorphisms Can Influence the Effect of Multicomponent Training on Blood Pressure, Nitrite Concentration and Physical Fitness in Prehypertensive and Hypertensive Older Adult Women

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