Endothelial nitric oxide synthase gene polymorphism in a cohort of Egyptian women with recurrent spontaneous miscarriage

Abulata, Nelly; AbdalWahab, Doaa F.; Osman, Omar

doi:10.1007/s00580-014-1957-z

Cited by 5 publications

(3 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The aim of the current work was to investigate the possible impact of combined genetic variants of VEGF G‐1154A gene and two eNOS genes: T‐786C promoter region and intron 4 VNTR in addition to TAFI C‐1040 T among Egyptian patients with RSA, which could be a novel highlight on the genetic interaction of the factors affecting angiogenesis, vasoconstrictive and fibrinolytic system, hoping for better understanding of such interactions and to find targeted therapies that can optimize plasma levels of such factors to ameliorate the incidence of RSA. All the studied genetic variants were significantly higher among the RSA patient group than the controls, except for TAFI C‐1040Tgenetic variant …”

Section: Discussionmentioning

confidence: 89%

“…All the studied genetic variants were significantly higher among the RSA patient group than the controls, except for TAFI C-1040Tgenetic variant. 7,23,24 Regarding VEGF-1154G/A genetic variant, a metaanalysis executed by Xu et al, 5 had reconsidered the possible link between VEGF genetic variants and the development of RSA and validated that −1154G/A (rs1570360), +936C/T (rs3025039), −634G/C (rs2010963) and −583T/C (rs3025020) genetic variants in the VEGF gene were associated with RSA risk. Furthermore, the analysis denoted that these genetic variants may be used as potential biomarkers for predicting individual vulnerability to RSA.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

The prevalence of combined vascular endothelial growth factor, endothelial nitric oxide synthase and thrombin‐activatable fibrinolysis inhibitor genetic polymorphisms among Egyptian patients with recurrent spontaneous abortion

Abulata

Shaheen

Osman

et al. 2019

J of Obstet and Gynaecol

Self Cite

View full text Add to dashboard Cite

Aim As angiogenesis is an essential step for chorionic villi formation. Vascular endothelial growth factor (VEGF) is essential for endothelial cell proliferation. Endothelial nitric oxide synthase (eNOS) is a powerful playmaker in hypoxia‐induced angiogenesis. Thrombin‐activatable fibrinolysis inhibitor (TAFI) regulates both fibrinolysis and inflammation. Genetic alterations of these factors may lead to recurrent spontaneous abortion (RSA). We aimed to investigate the combined genetic variants of VEGF G‐1154A and two eNOS genetic variants: T‐786C promoter region and intron 4 variable number of tandom repeats in addition to TAFI C‐1040T among RSA patients. Methods The study included 50 patients with RSA and 50 healthy controls. Polymerase chain reaction and restriction fragment length polymorphism were used for genotyping. Results Both genetic alterations of eNOS confirmed at least a sixfold increase of RSA risk. Interestingly, they were associated with TAFI C‐1040Tgenetic variant in 21 patients, eight of them had both studied eNOS genetic alterations and TAFI C‐1040Tgenetic variant, while each eNOS genetic variant associated with TAFI C‐1040Tconfirmed an almost one and half fold increase risk of RSA. Conclusion These findings highlighted the role of eNOS and nitric oxide metabolism in RSA and opened the gate to investigate the interaction of vasoconstrictive and fibrinolytic inhibitor systems.

show abstract

Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

The prevalence of combined vascular endothelial growth factor, endothelial nitric oxide synthase and thrombin‐activatable fibrinolysis inhibitor genetic polymorphisms among Egyptian patients with recurrent spontaneous abortion

Abulata

Shaheen

Osman

et al. 2019

J of Obstet and Gynaecol

Self Cite

View full text Add to dashboard Cite

show abstract

“…[26]. An investigation on a group of Egyptian women exposed a signi cantly association between RPL and this variant [27]. A meta-analysis study reported that this variant has a signi cantly correlation with the RPL risk [28].…”

Section: Discussionmentioning

confidence: 99%

Investigation of the association between eNOS gene promoter polymorphism (-786 T>C) and idiopathic recurrent pregnancy loss in Iranian women

Jalili

Asadollahi

Seifati

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Repeated Pregnancy Loss (RPL) is defined as 2 or more consecutive spontaneous losses of pregnancy before 20 weeks. Some genetic polymorphisms such as endothelial nitric oxide synthase (eNOS) gene, which lead to the synthesis of nitric oxide, could be the reasons for RPL. This case-control study was investigated the frequency of -786 T>C variant in eNOS gene promoter in Iranian women with RPL. Methods : Blood samples were obtained from 100 unrelated women affected by recurrent pregnancy loss and 100 unaffected women as controls. Genomic DNA was extracted and -786 T>C polymorphism in eNOS gene promoter investigated by PCR-RFLP method in all of the samples. Statistical analysis in the group patients and controls were performed by chi-square test and P-values of <0.05 were considered significant.Results: Frequency of homozygous TT was 40% in cases and 46% in control group and frequency of CC was 6% in cases and 5% in the control group and frequency heterozygote TC was 54% in cases and 46% in control group. Genotype frequencies between the two groups showed no significant differences (P>0/05).Conclusion: The result of this study showed that this polymorphism is not more frequent in recurrent pregnancy loss in this population.

show abstract

Gene polymorphism associated with endothelial nitric oxide synthase (4VNTR, G894T, C786T) and unexplained recurrent spontaneous abortion risk

Zhao

et al. 2019

Medicine

View full text Add to dashboard Cite

To evaluate the association between endothelial nitric oxide synthase gene polymorphisms (4VNTR A/B, G894T, C786T) and risk of URSA.Related case-control studies were collected by computers. A meta-analysis was conducted using Stata 12.0 software to assess the strength of association.Altogether 37 articles were examining the relationship between endothelial nitric oxide synthase gene polymorphisms and URSA, among which sixteen (16) studies were related to 4VNTR, twelve (12) to G894T, and nine (9) to C786T, the study suggested that 4VNTR A/B polymorphism was closely connected with URSA risk under all gene models except for recessive model (AA vs. BB + AB). The integrated result which indicated the association between G894T gene mutation and URSA risk had been shown under homozygote (TT vs. GG; OR 1.585, 95%CI 1.175–2.138) and recessive models (TT vs. TG + GG; OR 1.530, 95%CI 1.142–2.052). Considering heterogeneity in the remaining gene models, subgroup analysis was performed on ethnicity, and the results showed that it was the dominant (TT + TG vs. GG; OR 1.585, 95%CI 1.175–2.138) and additive models (T vs. G; OR 1.727, 95%CI 1.372–2.175) of G894T in Asians and the heterozygote model (TG vs. GG; OR 1.015, 95%CI 0.846–1.217) in Caucasians that were associated with URSA (P < .05). Besides C786T gene was significantly connected with URSA under all models except for additive model (T vs. C).It is of great guiding significance for screening out and preventing URSA among high-risk women via testing on 4VNTR A/B, G894T, C786T eNOS under gene models mentioned above which are closely associated with URSA.

show abstract

Endothelial nitric oxide synthase gene polymorphism in a cohort of Egyptian women with recurrent spontaneous miscarriage

Cited by 5 publications

References 12 publications

The prevalence of combined vascular endothelial growth factor, endothelial nitric oxide synthase and thrombin‐activatable fibrinolysis inhibitor genetic polymorphisms among Egyptian patients with recurrent spontaneous abortion

The prevalence of combined vascular endothelial growth factor, endothelial nitric oxide synthase and thrombin‐activatable fibrinolysis inhibitor genetic polymorphisms among Egyptian patients with recurrent spontaneous abortion

Investigation of the association between eNOS gene promoter polymorphism (-786 T>C) and idiopathic recurrent pregnancy loss in Iranian women

Gene polymorphism associated with endothelial nitric oxide synthase (4VNTR, G894T, C786T) and unexplained recurrent spontaneous abortion risk

Contact Info

Product

Resources

About

Endothelial nitric oxide synthase gene polymorphism in a cohort of Egyptian women with recurrent spontaneous miscarriage

Cited by 5 publications

References 12 publications

The prevalence of combined vascular endothelial growth factor, endothelial nitric oxide synthase and thrombin‐activatable fibrinolysis inhibitor genetic polymorphisms among Egyptian patients with recurrent spontaneous abortion

The prevalence of combined vascular endothelial growth factor, endothelial nitric oxide synthase and thrombin‐activatable fibrinolysis inhibitor genetic polymorphisms among Egyptian patients with recurrent spontaneous abortion

Investigation of the association between eNOS gene promoter polymorphism (-786 T&gt;C) and idiopathic recurrent pregnancy loss in Iranian women

Gene polymorphism associated with endothelial nitric oxide synthase (4VNTR, G894T, C786T) and unexplained recurrent spontaneous abortion risk

Contact Info

Product

Resources

About

Investigation of the association between eNOS gene promoter polymorphism (-786 T>C) and idiopathic recurrent pregnancy loss in Iranian women