Endothelial lipase is a major determinant of HDL level

Ishida, Tatsuro; Choi, S.; Kundu, Ramendra K.; Hirata, Ken‐ichi; Rubin, Edward M.; Cooper, Allen D.; Quertermous, Thomas

doi:10.1172/jci16306

Cited by 269 publications

(134 citation statements)

References 26 publications

(12 reference statements)

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“…A previous study reported that transgenic overexpression of EL under the control of an endogenous promoter resulted in reduced HDL levels [12]. In addition, Jin et al [21] reported that inhibition of EL activity by antibodies resulted in increased HDL levels.…”

Section: Discussionmentioning

confidence: 99%

“…EL exists on the endothelium lining blood vessels, and EL mRNA has been detected in placenta, thyroid, coronary arteries, kidney, liver, lung, ovary, and testis. An important role of EL is to metabolize high-density lipoprotein cholesterol (HDL) [12]. Furthermore, it has been shown that EL mRNA and its activity are upregulated in endothelial cells by pro-inflammatory cytokines such as tumor necrosis factor-α (TNF-α) and interleukin (IL)-1β [13, 14].…”

Section: Introductionmentioning

confidence: 99%

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Putative Role of Endothelial Lipase in Dialysis Patients with Hypoalbuminemia and Inflammation

Fujii

Fukuda²,

Tanaka³

et al. 2008

Am J Nephrol

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Putative Role of Endothelial Lipase in Dialysis Patients with Hypoalbuminemia and Inflammation

Fujii

Fukuda²,

Tanaka³

et al. 2008

Am J Nephrol

Self Cite

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“…The 5 0 and 3 0 flanking DNA sequences of exon 1 of the mouse membrane bound O-acyltransferase domain containing 4 (Mboat4) gene were subcloned by PCR from the bacterial artificial chromosome (BAC) clone RP23-99B10 (Life Technologies). These sequences were ligated with a PGK-Neo cassette (Ishida et al 2003), so as to replace Mboat4 exon 1. A DT-A cassette (Yagi et al 1993) was inserted at the 3 0 terminus of the vector.…”

Section: Generation Of Goat-deficient Micementioning

confidence: 99%

Ghrelin O-acyltransferase knockout mice show resistance to obesity when fed high-sucrose diet

Kouno

Akiyama

Ito

et al. 2015

Journal of Endocrinology

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Ghrelin is an appetite-stimulating hormone secreted from stomach. Since the discovery that acylation of the serine-3 residue by ghrelin O-acyltransferase (GOAT) is essential for exerting its functions, GOAT has been regarded as an therapeutic target for attenuating appetite, and thus for the treatment of obesity and diabetes. However, contrary to the expectations, GOAT-knockout (KO) mice have not shown meaningful body weight reduction, under high-fat diet. Here, in this study, we sought to determine whether GOAT has a role in body weight regulation and glucose metabolism with a focus on dietary sucrose, because macronutrient composition of diet is important for appetite regulation. We found that peripherally administered acylated-ghrelin, but not unacylated one, stimulated sucrose consumption in a two-bottle-drinking test. The role of acylated-ghrelin in sucrose preference was further supported by the finding that GOAT KO mice consumed less sucrose solution compared with WT littermates. Then, we investigated the effect of dietary composition of sucrose on food intake and body weight in GOAT KO and WT mice. As a result, when fed on high-fat diet, food intake and body weight were similar between GOAT KO and WT mice. However, when fed on high-fat, high-sucrose diet, GOAT KO mice showed significantly reduced food intake and marked resistance to obesity, leading to amelioration of glucose metabolism. These results suggest that blockade of acylated-ghrelin production offers therapeutic potential for obesity and metabolic disorders caused by overeating of palatable food.

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“…Analysis of various human tissues has detected EL RNA in placenta, lung, liver, kidney, testis and abundantly in the thyroid in both human and mouse tissues [3,4]. In a transgenic mouse model of human endothelial lipase under control of its own promoter, Ishida et al [11] detected EL protein by immunohistochemistry on the surface of endothelium of the aorta and large vessels in the kidney, lung, and spleen as well as the microcirculation in the spleen and lung. EL is expressed by endothelial cells in culture as detected at both the mRNA level [3] and the protein level [12].…”

Section: Tissue Expression Of Endothelial Lipase and Its Implicationsmentioning

confidence: 99%

Endothelial Lipase: A Novel Drug Target for HDL and Atherosclerosis?

Badellino

Jin

Rader

2004

Lipases and Phospholipases in Drug Development

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Endothelial lipase is a major determinant of HDL level

Cited by 269 publications

References 26 publications

Putative Role of Endothelial Lipase in Dialysis Patients with Hypoalbuminemia and Inflammation

Putative Role of Endothelial Lipase in Dialysis Patients with Hypoalbuminemia and Inflammation

Ghrelin O-acyltransferase knockout mice show resistance to obesity when fed high-sucrose diet

Endothelial Lipase: A Novel Drug Target for HDL and Atherosclerosis?

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