Endothelial Keratoplasty: The Influence of Preoperative Donor Endothelial Cell Densities on Dislocation, Primary Graft Failure, and 1-Year Cell Counts

Abstract

Preoperative donor ECD was not associated with donor dislocation for any form of EK surgery. Tissue with donor cell counts below 2500 cells per square millimeter can attach, and tissue with donor cell counts above 2500 cells per square millimeter can detach. Higher preoperative donor ECD was not correlated with higher ECD at 1 year postoperatively. Surgeons' requests for donor tissue with an ECD above 2500 cells per square millimeter for DSAEK surgery for the purpose of avoiding dislocations, IPGF, or improving 1-year ECD are not supported by this data.

Descemet-stripping automated endothelial keratoplasty in eyes with toxic anterior segment syndrome after cataract surgery

“…In our study, cell loss continued throughout the postoperative follow-up, as found in other studies of DSAEK. 11,13,14 The decrease was 0.4% from 6 to 12 months in our study. The long-term ECC and the rate of decrease are important determinants of the prognosis in these patients.…”

Análise da qualidade das córneas doadas e do intervalo entre óbito, enucleação e preservação após a implantação de novas normas técnicas e sanitárias em Banco de Olhos Universitário

“…Al guns autores observaram que o intervalo entre o óbito e a preser vação pode estar associado à maior chance de defeito epitelial na córnea. Isso pode aumentar a exposição do tecido a traumatismos e à toxicidade de substâncias e medicamentos durante o período de pre servação, diminuindo sua qualidade para transplante (10) . Já Slettedal et al demonstraram a enorme capacidade regenerativa do epitélio corneal com células viáveis após sete dias do óbito (18) .…”

“…Intervalos prolongados entre ΔtOE e entre ΔtEP podem jus tificar a não liberação do tecido para transplantes ópticos (9,10) . Se gundo Farias et al, a qualidade dos tecidos está inalterada em até seis horas (11) .…”

Construction of a tissue-engineered human corneal endothelium and its transplantation in rabbit models

“…In the past two decades, several groups constructed TE-HCE successfully and performed transplantation in animal models (Ishino et al, 2004;Mimura et al, 2004;Lai et al, 2007;Götze et al, 2008;Hitani et al, 2008;Gruschwitz et al, 2010;Fan TJ et al, 2011;Fan et al, 2013). Unfortunately, the application of TE-HCE was hampered by graft failure due to immune rejections and substantial loss of HCE cells after various keratoplasty (Terry et al, 2008;Lass et al, 2010;Regis-Pacheco and Binder, 2014). After patients were transplanted with donated corneas with an average ECD of 2818 cells/mm 2 by posterior lamellar keratoplasty, the postoperative ECD averaged 2126 cells/mm 2 at 6 months, 1859 cells/mm 2 at 12 months, 1385 cells/mm 2 at 24 months, and 1047 cells/mm 2 at 36 months (Van Dooren et al, 2004).…”

“…TE-HCE has been successfully constructed using different types of corneal endothelial cells and scaffold carriers and transplanted into various animal models (Mimura et al, 2004;Götze et al, 2008;Gruschwitz et al, 2010), but graft failure due to donor endothelial cell density (ECD) or dislocation often occurs (Terry et al, 2008;Lass et al, 2010). Based on our previous achievements (Fan et al, 2010(Fan et al, , 2013Fan TJ et al, 2011), the present study was designed to construct a high density TE-HCE using nontransfected HCE cells at low passage as seeder cells and modified denuded amniotic membrane (mdAM) as a scaffold, and to evaluate its biological functions by corneal transplantation in rabbit models.…”